IL36A Mouse, His
Description
Biological Activity and Signaling
IL-36α signals through the IL-1 receptor-related protein 2 (IL-1 Rrp2) and IL-1 receptor accessory protein (IL-1 RAcP) . Key functional data:
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Binding Affinity: Binds recombinant mouse IL-1 Rrp2 in functional ELISA .
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Cytokine Induction: Induces IL-6 secretion in NIH-3T3 fibroblasts (ED₅₀: 3–18 ng/mL) .
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Inflammatory Role:
Inflammation Models
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Lung Inflammation: Intratracheal IL-36α instigation in mice induces neutrophil influx and upregulates IL-36R expression in CD11c⁺ cells .
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Skin Pathology: Overexpression in keratinocytes drives psoriatic-like epidermal hyperplasia .
Metabolic Studies
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Weight Regulation: Il36rn⁻⁄⁻ mice (lacking IL-36 receptor antagonist) exhibit reduced weight gain and improved glucose tolerance, suggesting IL-36α modulates adipose tissue inflammation .
Immune Cell Interactions
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Antigen-Presenting Cells: IL-36α activates lung CD11c⁺ cells to express costimulatory molecules (e.g., CD40) and enhance CD4⁺ T-cell proliferation .
Physical and Chemical Properties
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Solubility: Supplied as a 1 mg/mL solution in phosphate-buffered saline (pH 7.4) with 10% glycerol .
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Stability: Stable at 4°C for 2–4 weeks; long-term storage requires -20°C with carrier protein (e.g., 0.1% HSA/BSA) .
Research Implications
IL36A Mouse, His is a vital tool for studying IL-36α’s role in:
Product Specs
Product Science Overview
Interleukin-36 alpha (IL-36α), also known as IL-1F6, is a member of the interleukin-1 (IL-1) family of cytokines. It plays a crucial role in the immune response, particularly in the skin and epithelial tissues. IL-36α is involved in the activation of NF-kappa-B and MAPK signaling pathways, which are essential for generating an inflammatory response . This cytokine is known to participate in the production of other pro-inflammatory cytokines, including IL-12, IL-1β, IL-6, TNF-α, and IL-23 .
Recombinant IL-36α (Mouse, His Tag) is a laboratory-produced version of the natural cytokine, designed for research purposes. It is expressed in Escherichia coli (E. coli) and purified using conventional chromatography techniques . The recombinant protein is fused with a His-tag at the N-terminus, which facilitates its purification and detection.
The preparation of recombinant IL-36α involves several steps:
- Gene Cloning: The gene encoding IL-36α is cloned into an expression vector, which is then introduced into E. coli cells.
- Protein Expression: The E. coli cells are cultured under conditions that induce the expression of the recombinant IL-36α protein.
- Purification: The expressed protein is purified using chromatography techniques, taking advantage of the His-tag for affinity purification .
Recombinant IL-36α is typically analyzed using techniques such as SDS-PAGE and mass spectrometry (MS) to confirm its purity and molecular weight . The protein’s biological activity can be assessed by its ability to activate NF-kappa-B and MAPK signaling pathways in target cells, leading to the production of pro-inflammatory cytokines .
Recombinant IL-36α is used in various research applications, including:
- Studying Inflammatory Responses: Researchers use IL-36α to investigate its role in inflammatory diseases, such as psoriasis and other skin conditions .
- Drug Development: IL-36α is a target for developing new anti-inflammatory drugs.
- Cell Signaling Studies: The protein is used to study the signaling pathways involved in immune responses .
