IL5RA Human
Description
Overview of IL5RA Human
IL5RA (Interleukin-5 Receptor Alpha Subunit), also known as CD125, is a critical component of the heterodimeric IL-5 receptor. It binds specifically to IL-5 and pairs with a shared beta subunit (CSF2RB) to mediate signaling for eosinophil survival, differentiation, and chemotaxis . The protein is expressed as a glycosylated polypeptide chain (331 amino acids, ~37.8 kDa) and is produced recombinantly in Sf9 Baculovirus cells for research and therapeutic applications .
Genetic Polymorphisms and Disease Associations
IL5RA polymorphisms influence eosinophil activation and immune responses, with implications in asthma, myeloma, and aspirin-exacerbated respiratory disease (AERD).
In AERD patients, the −5993A allele correlates with elevated serum IgE to staphylococcal enterotoxin A, suggesting a role in mast cell and eosinophil activation .
Therapeutic Targeting of IL5RA
IL5RA is a key target in eosinophilic disorders due to its role in IL-5 signaling. Monoclonal antibodies (e.g., benralizumab) block IL5RA, depleting eosinophils via antibody-dependent cellular cytotoxicity .
Asthma and Allergic Inflammation
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IL5RA polymorphisms (e.g., −5993A>G) increase promoter activity, elevating IL5RA expression and eosinophil recruitment .
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Soluble IL5RA isoforms modulate IL-5 bioavailability, influencing IgE production and atopy .
Myeloma and Immunogenic Cell Death (ICD)
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IL5RA upregulation in multiple myeloma (MM) correlates with progression and resistance to therapy. It enriches pathways like PI3K-Akt and natural killer cell cytotoxicity, promoting tumor survival .
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IL5RA interacts with ICD-related genes (e.g., CALR, CASP8), suggesting a role in regulating immune responses against cancer .
Eosinophilic Disorders
Product Specs
Interleukin 5 Receptor Subunit Alpha, Interleukin 5 Receptor, Alpha, IL-5 Receptor Subunit Alpha, IL-5R Subunit Alpha, CD125 Antigen, CDw125, IL5R, Interleukin-5 Receptor Subunit Alpha, Interleukin-5 Receptor Alpha Chain, Interleukin 5 Receptor Type 3, IL-5R-Alpha, HSIL5R3, IL-5RA, CD125, Interleukin-5 receptor subunit alpha, IL-5 receptor subunit alpha, IL-5R subunit alpha, IL-5R-alpha, IL-5RA.
ADPDLLPDEK ISLLPPVNFT IKVTGLAQVL LQWKPNPDQE QRNVNLEYQV KINAPKEDDY ETRITESKCV TILHKGFSAS VRTILQNDHS LLASSWASAE LHAPPGSPGT SIVNLTCTTN TTEDNYSRLR SYQVSLHCTW LVGTDAPEDT QYFLYYRYGS WTEECQEYSK DTLGRNIACW FPRTFILSKG RDWLAVLVNG SSKHSAIRPF DQLFALHAID QINPPLNVTA EIEGTRLSIQ WEKPVSAFPI HCFDYEVKIH NTRNGYLQIE KLMTNAFISI IDDLSKYDVQ VRAAVSSMCR EAGLWSEWSQ PIYVGNDEHK PLREWHHHHH H.
Product Science Overview
Interleukin-5 Receptor Alpha (IL-5Rα), also known as CD125, is a protein that plays a crucial role in the immune system, particularly in the regulation of eosinophils. Eosinophils are white blood cells that are involved in the body’s response to allergens and parasitic infections. The recombinant form of this receptor is used in various research and therapeutic applications.
IL-5Rα is a subunit of the IL-5 receptor, which is a heterodimer consisting of the alpha subunit (IL-5Rα) and the beta subunit (βc). The alpha subunit is responsible for binding to the IL-5 cytokine, while the beta subunit is shared with other cytokine receptors and is involved in signal transduction .
The IL-5 receptor is primarily expressed on the surface of eosinophils and basophils. Upon binding to IL-5, the receptor activates various intracellular signaling pathways, leading to the proliferation, differentiation, and survival of eosinophils .
Recombinant IL-5Rα is produced using recombinant DNA technology, where the gene encoding the human IL-5Rα protein is inserted into a suitable expression system, such as Chinese Hamster Ovary (CHO) cells. This allows for the large-scale production of the protein for research and therapeutic purposes .
The recombinant form of IL-5Rα is often used in studies to understand the receptor’s role in immune responses and to develop targeted therapies for conditions involving eosinophils, such as asthma and eosinophilic esophagitis .
- Research: Recombinant IL-5Rα is used in various research applications to study the signaling pathways and biological functions of the IL-5 receptor. It helps in understanding the mechanisms underlying eosinophil-related diseases and in identifying potential therapeutic targets .
- Therapeutics: The recombinant receptor is also used in the development of therapeutic agents. For example, monoclonal antibodies targeting IL-5Rα have been developed to treat eosinophilic disorders by blocking the interaction between IL-5 and its receptor .
