IL6 Human, Sf9
Description
Key Molecular Features
Production Process in Sf9 Cells
IL6 Human, Sf9 is synthesized through a baculovirus expression system:
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Cloning: Human IL-6 gene is inserted into a baculovirus transfer vector.
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Transfection: Linearized baculovirus DNA and transfer vector are introduced into Sf9 cells.
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Virus Amplification: Recombinant baculovirus is propagated in Sf9 cultures.
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Protein Secretion: IL-6 is expressed and secreted into the medium.
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Purification: Chromatographic techniques isolate the His-tagged protein.
Advantages Over E. coli Systems
Biological Properties and Functional Roles
IL6 Human, Sf9 exhibits pleiotropic effects:
Immune Regulation
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B Cell Differentiation: Induces plasma cell formation and immunoglobulin secretion .
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T Cell Activation: Drives Th17 polarization and inhibits Th1 responses via STAT3/SOCS3 pathways .
Inflammation and Viral Responses
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Antiviral Activity: Suppresses hepatitis B virus (HBV) replication by blocking receptor expression (NTCP) .
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Immunosuppressive Effects: Excess IL-6 impairs CD8+ T-cell cytotoxicity (via SOCS3-mediated inhibition of STAT4) .
Experimental Uses
Therapeutic Potential
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Autoimmune Diseases: Targeting IL-6 trans-signaling with sgp130Fc to mitigate inflammation .
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Hematopoietic Disorders: Leveraging IL-6’s role in stem cell self-renewal .
Production Challenges
| Issue | Solution |
|---|---|
| Low Yield | Baculovirus titer optimization; media formulation refinement |
| Protease Degradation | Inclusion of protease inhibitors; rapid purification post-harvest |
Comparative Analysis: IL6 Human, Sf9 vs. Mouse IL6, Sf9
Viral Infections
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HBV: IL6 Human, Sf9 inhibits viral entry by downregulating NTCP .
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HIV: Elevated IL-6 correlates with residual viremia and T-cell dysfunction .
Inflammatory Diseases
Product Specs
BSF2, HGF, HSF, IFNB2, IL-6.
VPPGEDSKDV AAPHRQPLTS SERIDKQIRY ILDGISALRK ETCNKSNMCE SSKEALAENN
LNLPKMAEKD GCFQSGFNEE TCLVKIITGL LEFEVYLEYL QNRFESSEEQ ARAVQMSTKV
LIQFLQKKAK NLDAITTPDP TTNASLLTKL QAQNQWLQDM TTHLILRSFK EFLQSSLRAL RQMHHHHHH.
Product Science Overview
Interleukin-6 (IL-6) is a multifunctional cytokine that plays a crucial role in immune regulation, inflammation, and hematopoiesis. It is involved in various physiological processes, including the differentiation of B-cells into immunoglobulin-secreting cells, the growth of myeloma and plasmacytoma cells, and the induction of acute phase reactants in hepatocytes .
Human Recombinant IL-6 is produced using the Sf9 insect cell system, which is derived from the Spodoptera frugiperda (fall armyworm). This system utilizes baculovirus vectors to efficiently express recombinant proteins. The production process involves cloning the human IL-6 gene into a baculovirus transfer vector, which is then co-transfected with linearized baculovirus DNA into Sf9 cells .
The recombinant IL-6 produced in Sf9 cells is a single glycosylated polypeptide chain containing 189 amino acids, with a molecular mass of approximately 21.6 kDa. It is fused to a 6 amino acid His-Tag at the C-terminus and purified using proprietary chromatographic techniques. The protein is typically formulated in a phosphate-buffered saline (PBS) solution with 10% glycerol for stability .
Human Recombinant IL-6 exhibits significant biological activity. It has been shown to induce the proliferation of TF-1 human erythroleukemic cells, with an effective dose (ED50) ranging from 0.2 to 0.8 ng/mL . This activity underscores its importance in various biological processes and its potential therapeutic applications.
The production of Human Recombinant IL-6 using the Sf9 cell system provides a versatile tool for investigating its biological functions and potential therapeutic applications. IL-6 is a target of intensive research due to its pivotal role in immune regulation and inflammation. Understanding its mechanisms can lead to the development of novel therapeutic strategies for various diseases, including autoimmune disorders, cancers, and inflammatory conditions .
