sIL6R Human, Sf9
Description
sIL6R produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain (20-365 a.a.) and fused to a 6 aa His Tag at C-terminus containing a total of 355 amino acids and having a molecular mass of 39.6kDa.
sIL6R purified by proprietary chromatographic techniques.
Product Specs
The IL-6 receptor complex consists of two membrane glycoproteins: a low-affinity receptor and a signal-transducing component. Soluble IL6R (sIL6R) can be found in the urine of healthy adults, the serum of HIV-positive individuals, and the cell culture supernatants of stimulated peripheral blood mononuclear cells (PBMCs). This soluble form arises from either proteolytic cleavage of the membrane-bound receptor or from the expression of a splice variant.
Recombinant human sIL6R produced in Sf9 insect cells is a single, glycosylated polypeptide chain encompassing amino acids 20-365 of the mature protein and fused to a C-terminal 6-histidine tag. This protein has a molecular mass of 39.6 kDa. sIL6R undergoes purification using proprietary chromatographic techniques.
The sIL6R protein solution is provided at a concentration of 0.25 mg/ml in a buffer containing 10% glycerol and phosphate-buffered saline (pH 7.4).
Purity is determined to be greater than 90.0% by SDS-PAGE analysis.
The biological activity of sIL6R is assessed by its ability to inhibit the proliferation of M1 mouse myeloid leukemia cells in the presence of human IL-6. The ED50 value, representing the effective concentration at which 50% inhibition is observed, falls within the range of 20 ng/ml when tested with human IL-6.
IL6R-alpha, CD126, IL-6R 1, CD126 antigen, IL6RA, Interleukin 6 receptor, Interleukin 6 receptor alpha subunit, Interleukin-6 receptor alpha chain precursor, B cell stimulatory factor-2, Membrane glycoprotein 80, gp80, IL-6R, MGC104991.
ADPLAPRRCP AQEVARGVLT SLPGDSVTLT CPGVEPEDNA TVHWVLRKPA AGSHPSRWAG MGRRLLLRSV QLHDSGNYSC YRAGRPAGTV HLLVDVPPEE PQLSCFRKSP LSNVVCEWGP RSTPSLTTKA VLLVRKFQNS PAEDFQEPCQ YSQESQKFSC QLAVPEGDSS FYIVSMCVAS
SVGSKFSKTQ TFQGCGILQP DPPANITVTA VARNPRWLSV TWQDPHSWNS SFYRLRFELR YRAERSKTFT TWMVKDLQHH CVIHDAWSGL RHVVQLRAQE EFGQGEWSEW SPEAMGTPWT ESRSPPAENE VSTPMQALTT NKDDDNILFR DSANATSLPV QDSSSVPLPH HHHHH
Product Science Overview
Interleukin-6 (IL-6) is a multifunctional cytokine that plays a crucial role in immune response, inflammation, and hematopoiesis. The IL-6 receptor (IL-6R) is composed of two subunits: the IL-6R alpha chain (IL-6Rα) and the signal-transducing component gp130. The soluble form of IL-6R (sIL-6R) can bind to IL-6 and form a complex that interacts with gp130, leading to signal transduction even in cells that do not express membrane-bound IL-6R. This article delves into the background of the IL-6 Soluble Receptor (Human Recombinant, Sf9), its production, and its significance in research and clinical applications.
The IL-6 Soluble Receptor (Human Recombinant, Sf9) is produced using the baculovirus expression system in Sf9 insect cells. This method ensures high yield and proper folding of the recombinant protein. The recombinant sIL-6R is a non-glycosylated polypeptide chain containing 338 amino acids, with a molecular weight of approximately 42.25 kDa .
The production process involves the following steps:
- Gene Cloning: The gene encoding the IL-6Rα is cloned into a baculovirus transfer vector.
- Transfection: Sf9 insect cells are transfected with the recombinant baculovirus.
- Protein Expression: The transfected cells express the recombinant sIL-6R, which is then secreted into the culture medium.
- Purification: The sIL-6R is purified using chromatographic techniques to achieve high purity levels (>90%) as determined by SDS-PAGE .
The soluble form of IL-6R can be produced through two primary mechanisms:
The sIL-6R plays a pivotal role in IL-6 signaling by extending the range of IL-6 activity to cells that do not express membrane-bound IL-6R. This process, known as trans-signaling, is crucial in various physiological and pathological conditions, including:
- Immune Response: sIL-6R enhances the immune response by promoting the activation and differentiation of T cells and B cells .
- Inflammation: Elevated levels of sIL-6R are associated with chronic inflammatory diseases such as rheumatoid arthritis and inflammatory bowel disease .
- Cancer: sIL-6R is implicated in tumor progression and metastasis by promoting the survival and proliferation of cancer cells .
The IL-6 Soluble Receptor (Human Recombinant, Sf9) is widely used in research to study the IL-6 signaling pathway and its implications in various diseases. It also holds potential therapeutic applications, such as:
