IL8 Recombinant Monoclonal Antibody
Shipped with Ice Packs 
In Stock 
Description
Definition and Production of IL8 Recombinant Monoclonal Antibodies
IL8 recombinant mAbs are fully human or humanized antibodies designed to bind and block IL-8’s interaction with CXCR1/CXCR2 receptors. These antibodies are produced through advanced biotechnological methods:
-
XenoMouse Technology: Mice engineered with human immunoglobulin loci (e.g., ABX-IL8) generate fully human antibodies with high specificity .
-
Recombinant DNA Methods: Synthetic genes encoding IL-8-binding antibodies are cloned into mammalian cell lines (e.g., CSB-RA582227A0HU) .
Key antibodies include:
| Antibody Name | Source/Host | Application | Cross-Reactivity |
|---|---|---|---|
| ABX-IL8 | Human | Melanoma | Human |
| HuMax-IL8 | Human | Solid tumors | Human |
| MAB208 | Mouse | ELISA, WB | Porcine IL-8 (100%) |
| CSB-RA582227A0HU | Rabbit-derived recombinant | Flow cytometry | Human |
| 60141-1-Ig | Mouse | WB, IHC | Human |
Mechanism of Action
IL8 mAbs block IL-8’s binding to its receptors, disrupting downstream signaling pathways:
-
Anti-Angiogenic Effects: Inhibit endothelial cell migration and tubule formation, reducing tumor vascularization .
-
Anti-Metastatic Activity: Suppress matrix metalloproteinase-2 (MMP-2) expression, limiting tumor invasion .
-
Autophagy and CSC Suppression: Reduce LC3B (autophagy marker) and CD44 (cancer stem cell marker) in breast cancer models .
-
Immune Modulation: Decrease brachyury expression, reversing epithelial-to-mesenchymal transition (EMT) and enhancing immune-mediated tumor lysis .
Therapeutic Applications in Oncology
IL8 mAbs show promise in treating IL-8-driven cancers:
Melanoma
ABX-IL8 significantly inhibits subcutaneous tumor growth and metastasis in nude mice by:
Breast Cancer
Anti-IL8 mAbs suppress:
| Parameter | Effect | Source |
|---|---|---|
| Autophagy (LC3B) | ↓ Significant reduction in tumors | |
| Cancer Stem Cells (CD44) | ↓ Expression in tumor tissues | |
| Tumor Microenvironment | ↓ IL-8-driven oncogenic signaling |
Colorectal Cancer
IL8-CXCR2 axis blockade restores CD8+ T-cell function by upregulating TIM3, enhancing anti-tumor immunity .
Research and Diagnostic Applications
IL8 mAbs are widely used in preclinical studies:
Neutralization Capacity:
MAB208 neutralizes IL-8-induced chemotaxis (ND₅₀: 0.08–0.4 µg/mL) in CXCR2-transfected BaF3 cells .
Clinical Trials and Development
| Antibody | Trial Phase | Target Population | Key Outcomes |
|---|---|---|---|
| HuMax-IL8 (BMS-986253) | Phase I | Solid tumors | Safety and pharmacokinetics assessed; no severe adverse events reported |
Challenges and Future Directions
-
Immunogenicity: Fully human antibodies (e.g., ABX-IL8) reduce immune responses compared to chimeric/humanized counterparts .
-
Combination Therapies: Synergies with anti-angiogenic drugs (e.g., bevacizumab) or checkpoint inhibitors are under investigation .
-
Biomarker Identification: IL-8 expression levels and CXCR2 status may predict treatment response .
Product Specs
Buffer
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Description
The IL8 recombinant monoclonal antibody is produced through in vitro processes using synthetic genes. The IL8 antibody genes are initially extracted from B cells isolated from immunoreactive rabbits. These genes are then amplified and cloned into appropriate phage vectors. These vectors are subsequently introduced into mammalian cell lines, facilitating the production of functional antibodies. The IL8 recombinant monoclonal antibody is then purified through affinity chromatography. Following rigorous verification, the antibody is suitable for use in ELISA and FC applications to detect the human IL8 protein.
IL-8 is a pro-inflammatory chemokine that plays a crucial role in the immune response and inflammatory processes. Its functions include the recruitment and activation of immune cells, induction of inflammation, angiogenesis, and immune cell trafficking. While IL-8 is essential for the body's defense against infections and tissue repair, dysregulation of its activity can contribute to inflammatory diseases and cancer.
Form
Liquid
Lead Time
Generally, we can ship the products within 1-3 working days after receiving your order. Delivery time may vary depending on the purchasing method or location. Please consult your local distributors for specific delivery time details.
Synonyms
Interleukin-8 (IL-8) (C-X-C motif chemokine 8) (Chemokine (C-X-C motif) ligand 8) (Emoctakin) (Granulocyte chemotactic protein 1) (GCP-1) (Monocyte-derived neutrophil chemotactic factor) (MDNCF) (Monocyte-derived neutrophil-activating peptide) (MONAP) (Neutrophil-activating protein 1) (NAP-1) (Protein 3-10C) (T-cell chemotactic factor) [Cleaved into: MDNCF-a (GCP/IL-8 protein IV) (IL8/NAP1 form I), Interleukin-8 ((Ala-IL-8)77) (GCP/IL-8 protein II) (IL-8(1-77)) (IL8/NAP1 form II) (MDNCF-b), IL-8(5-77), IL-8(6-77) ((Ser-IL-8)72) (GCP/IL-8 protein I) (IL8/NAP1 form III) (Lymphocyte-derived neutrophil-activating factor) (LYNAP) (MDNCF-c) (Neutrophil-activating factor) (NAF), IL-8(7-77) (GCP/IL-8 protein V) (IL8/NAP1 form IV), IL-8(8-77) (GCP/IL-8 protein VI) (IL8/NAP1 form V), IL-8(9-77) (GCP/IL-8 protein III) (IL8/NAP1 form VI)], CXCL8, IL8
Target Names
Uniprot No.
Target Background
Function
IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It also participates in neutrophil activation. Various cell types release IL-8 in response to an inflammatory stimulus. IL-8(6-77) exhibits a 5-10-fold higher activity on neutrophil activation compared to IL-8(1-77). IL-8(5-77) demonstrates increased activity on neutrophil activation, while IL-8(7-77) exhibits a higher affinity to receptors CXCR1 and CXCR2 compared to IL-8(1-77), respectively.
Gene References Into Functions
- VEGF and IL-8 play a prominent role in the pathogenesis of early forms of rosacea and the hemostasis system. PMID: 29578433
- Elevated LIFR levels in colorectal cancer (CRC) facilitate proliferation and migration of endothelial cells, resulting in increased angiogenic activity. Moreover, IL-8 has been identified as a key player in LIFR-induced angiogenesis. IL-8 levels correlate with LIFR levels in CRC tissues, and depletion of IL-8 leads to a reduction in the angiogenic activity of LIFR in CRC cells. PMID: 29751081
- The PKC-delta isoform plays a crucial role in the Tat-TLR4 signaling pathway, activating NF-kappaB and CXCL8 production. PMID: 28539656
- CXCL8 is a target of miR-204, and suppression of miR-204 cannot increase cell viability, migration, invasion, and EMT procedure when CXCL8 is silenced. PMID: 29402343
- Interleukin 8 - 845 T/C and + 781 C/T polymorphisms were analyzed. For the + 781C/T locus, in the dominant genetic model, a significant difference was observed between TT vs. CC + CT genotypes, indicating a significant protective role against periodontitis disease. A positive association was found between the distribution of IL8 - 845 T/C alleles and the risk of periodontitis disease. The C allele of IL-8 - 845 increased the risk of periodontitis disease. PMID: 30078118
- These results suggest a direct involvement of IL-8-CXCR1/2 axes in GBM progression by promoting both cell proliferation and invasion, as well as indirectly by promoting neovascularization in the form of vascular mimicry. PMID: 30086759
- These findings indicate that AKIP1 is crucial in cervical cancer angiogenesis and growth by elevating the levels of the NF-kappaB-dependent chemokines CXCL1, CXCL2, and CXCL8. PMID: 29520695
- The extramembranous domain of HofQ (emHofQ) has been shown to interact with various cytokines, with IL-8 exhibiting the strongest interaction. PMID: 30088437
- The protein expression levels of IL-8 were significantly decreased in SZ patients, but no significant difference in the mRNA levels of IL-8 was observed between SZ patients and NC subjects. PMID: 28476335
- The immune system process is indispensable in the progression of disease in the colon, and identifies that IL-8 and MMP-9 play potential critical roles in the progression. PMID: 30074183
- IL-8 production was significantly enhanced following treatment with both IL-17A and CSE, while treatment with either IL-17A or CSE alone caused only a slight increase in IL-8 production. PMID: 29463070
- Research has identified IL-8 as a positive regulator of homotypic CIC formation through enhancing intercellular adhesion. PMID: 30021676
- V2O5 induction of CXCL8 and CXCL11 chemokines may lead to the emergence and perpetuation of an inflammatory reaction within the dermal tissue. Further studies are required to evaluate dermal integrity and manifestations in individuals occupationally exposed or living in polluted areas. PMID: 29901202
- Study findings suggest that PAR2 could play a vital role in gastroesophageal reflux disease (GERD) pathogenesis, indicating that even repeated short-term exposure to weakly acidic conditions leads to the upregulation of PAR2 and subsequent activation of the intense IL-8 release in the esophageal mucosa, initiating a mucosal immune response in GERD. PMID: 29672302
- Considering that IL-8, MIP-1beta, and MCP-1 are chemokines that play significant roles in the recruitment of immunocompetent cells for immune defense and tumor cell clearance, the observed lower levels of these markers with increasing PM2.5 exposure may provide insight into the mechanism by which DEE promotes lung cancer. PMID: 29023999
- These results suggest that stemness induction in SKOV3 cells by macrophages co-cultured with SKOV3-derived OCSLCs involves IL-8/STAT3 signaling. PMID: 29656182
- IL-8-251T>A (rs4073) Polymorphism is associated with gastric Cancer. PMID: 30275190
- The expression level of CXCL8 has a positive relationship with recurrence probability in Acute myeloid leukemia. PMID: 29596823
- These data were in close agreement with the reduced cell migration and colony formation. Results from the present study suggest that reparixin and SCH527123 may be promising therapeutic agents for the treatment of pancreatic cancer by inhibiting the IL8/CXCR1/2 signaling cascade. PMID: 29749433
- A urinary IL-8 level of less than 61.25 pg/ml is more sensitive for predicting complete remission in idiopathic membranous nephropathy patients. PMID: 29415357
- Berberine inhibited the expression of MCP-1 and IL-8 induced by LPS. PMID: 28852897
- Regarding the IL-8 promoter T - 251A, the TA and AA genotypes were associated with significantly decreased risks of nasopharyngeal carcinoma (NPC) in a Taiwanese population compared with the wild-type TT genotype. The mRNA and protein expression levels for NPC tissues revealed no significant associations among the 20 NPC samples with different genotypes. PMID: 30200105
- IL-8 +781 T/C polymorphism is associated with severe Clostridium difficile infection. PMID: 29203364
- ShRNA-mediated down-regulation of CXCL8 resulted in the inhibition of cell proliferation, viability, and invasion in vitro and a near complete growth reduction of the tumor in vivo. PMID: 29679563
- CSF IL-8 concentrations were significantly elevated in CNS tumor patients compared to non-tumoral individuals. AUC for CSF IL-8 was higher than for its index (CSF IL-8/serum IL-8). PMID: 29086194
- High IL8 expression is associated with melanoma. PMID: 29286146
- Lipo-CPFX, but not CPFX, retained the anti-IL-8 releasing activity. PMID: 29337216
- The results indicate a significant contribution of IL8 to the survival of hormonal-dependent early-stage breast cancer patients and an association with established parameters such as estrogen receptors/progesterone receptor and HER2. PMID: 28569250
- The frequency of non-classical monocytes expressing CXCL8 was increased in systemic sclerosis patients, and monocytes expressing CXCL8 PMID: 29127442
- Interactions between intermediate molecular mass hyaluronan and CD44 enhanced normal PMN phagocytosis and IL-8 production. PMID: 28730511
- Serum levels in active vitiligo were significantly elevated compared to those in stable vitiligo patients. PMID: 29115683
- High IL8 expression is associated with pancreatic adenocarcinoma. PMID: 29205349
- Compared to controls, the interleukin (IL)-8 A/A genotype was more common in acute pancreatitis (AP). PMID: 29215544
- The presence of neither the first transmembrane helix of the receptor nor the lipid bilayer significantly affected the interactions of IL-8 with Binding Site-I of CXCR1. PMID: 29143165
- CXCL8 is highly expressed in cervical cancer tissues and cell lines, and correlated with malignant status and prognosis in cervical cancer patients. PMID: 28883082
- In conclusion, to our knowledge, this is the first study in the association of rs4073 and rs2227306 polymorphisms with childhood asthma risk in the Tunisian population. PMID: 28993876
- Results show that IL8 expression level is regulated by APE1, which activates NF-KB. PMID: 27388124
- Aberrant miR-520c-3p expression may lead to reduced IL-8 expression and promote the mesenchymal phenotype in breast cancer cells, thereby increasing invasive growth. PMID: 29048659
- Increased levels of IL-8 are associated with factors of worse prognosis in ovarian cancer. PMID: 28872976
- Significantly elevated blood levels of IL-8 in myelodysplastic syndrome patients. PMID: 28856536
- Elevated concentrations of CXCL13, CXCL8, and CXCL10 or their increasing CSF/serum ratios may be potential biomarkers of neurosyphilis. PMID: 27650493
- Results indicate the significant role of PRL-3 in glycolysis metabolism by improving IL-8 secretion in colorectal cancer cells, and PRL-3-mediated glycolysis contributes to the promotion of cancer metastasis. PMID: 28791350
- Changes in serum IL-8 levels could be used to monitor and predict clinical benefit from immune checkpoint blockade in melanoma and NSCLC patients. PMID: 28595336
- Lung cancer patients exhibited significantly lower levels of serum VEGF (1.9 fold) and IL-8 (~9 fold) compared to COPD patients. VEGF levels were significantly higher (2.6 fold) in metastatic than non-metastatic cancer patients. An increase in MMP-9 (~1.6 fold) levels was observed in lung cancer patients. PMID: 27811960
- CXCL1/8 secreted by adipose-derived mesenchymal stem cells could promote breast cancer angiogenesis. PMID: 28514506
- Our meta-analysis suggests that the IL-8 rs4073, A2767T, T11722T2, rs2234671, rs2230054, rs1126579, rs2227306, rs2227307, rs2227532, and T-738A polymorphisms are not associated with periodontitis, while the IL-8 C1633T and rs1126580 polymorphisms may elevate the susceptibility of periodontitis based on the currently available evidence. PMID: 28446725
- An analogue of human CXCL8, CXCL8(3-72)K11R/G31P (hG31P) has been developed. PMID: 28754019
- Microcystin-LR exhibits an inflammation-triggered property via IL-8/CXCR2 signaling. PMID: 29197248
- A high IL-8 content in urine sampled on day 1 after renal transplantation was positively correlated with the activity of metalloproteinase-9 in urine. This evidence demonstrates that both chemokines cooperate in ischemia-reperfusion injuries in transplanted kidneys. PMID: 28494217
- We discovered that IL-8 secreted from decidual stromal cells is a key cytokine enhancing the invasiveness of trophoblasts. PMID: 28328096
Database Links
Protein Families
Intercrine alpha (chemokine CxC) family
Subcellular Location
Secreted.
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
