INHBB Recombinant Monoclonal Antibody
Description
Recombinant INHBB antibodies are synthesized through:
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Phage Display: Screening combinatorial libraries for high-affinity binders .
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Hybridoma Sequencing: Cloning variable regions from existing murine/rabbit hybridomas .
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Mammalian Transient Expression: HEK293F/CHO cells for scalable production .
Advantages over Traditional Antibodies:
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Batch Consistency: Defined genetic sequences eliminate hybridoma instability .
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High Specificity: Engineered via methods like affinity maturation .
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Ethical Production: No reliance on animal sera or hybridoma cell lines .
ELISA and Functional Assays
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Improved Sensitivity: Recombinant 46A/F antibody eliminates sample pre-treatment (e.g., H₂O₂ oxidation) required for earlier assays .
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Cross-Reactivity: <0.5% with inhibin A, resolved through optimized epitope recognition .
Western Blot and Immunohistochemistry
Therapeutic and Angiogenesis Studies
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Tumor Angiogenesis: INHBB promotes endothelial cell proliferation via TGF-β receptors (endoglin/ALK1), linked to ovarian and renal cancer progression .
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Diagnostic Markers: Elevated INHBB correlates with poor survival in TP53-mutated ovarian cancers .
Key Research Findings
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Assay Simplification: Recombinant 46A/F antibody eliminates overnight incubation and sample pretreatment, enabling rapid inhibin B quantification .
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Tumor Microenvironment: INHBB-driven angiogenesis in ovarian cancers is mediated by endothelial endoglin/ALK1 signaling, suggesting therapeutic targets .
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Cross-Reactivity: Earlier reports of 0.5% inhibin A cross-reactivity were reevaluated to <0.1% using high-purity recombinant standards .
Challenges and Considerations
Product Specs
The INHBB recombinant monoclonal antibody is produced through a meticulously designed in vitro process. Initially, INHBB antibody genes are extracted from B cells obtained from immunoreactive rabbits. These genes are subsequently amplified and incorporated into phage vectors. These vectors are then introduced into mammalian cell lines, facilitating the generation of functional antibodies in substantial quantities. The resulting INHBB recombinant monoclonal antibody is purified from the culture supernatant of the transfected cell lines utilizing affinity chromatography. This antibody demonstrates reactivity with human INHBB protein and has been rigorously validated for use in ELISA and FC applications.
INHBB plays a pivotal role in the formation of inhibin and activin, two hormone complexes that exert significant regulatory influence on the endocrine and reproductive systems. Moreover, they contribute to various aspects of embryonic development and tissue homeostasis. These multifaceted functions are crucial for maintaining hormonal equilibrium and ensuring the proper functioning of the reproductive system.
Target Background
Inhibins and activins exert opposing actions on the secretion of follitropin by the pituitary gland. Inhibins inhibit, while activins activate, this process. These hormone complexes are involved in regulating a diverse array of biological functions, including hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development, and bone growth. The specific functions of inhibins and activins are dependent on their subunit composition. Notably, inhibins generally counter the effects of activins.
- Studies have shown that INHBB expression is reduced in nasopharyngeal carcinoma (NPC) tissues and is associated with lymph node metastasis. Further investigations reveal that INHBB can suppress anoikis resistance and migration of NPC cells through the TGF-beta signaling pathway. Additionally, it decreases p53 overexpression, suggesting its potential as a biomarker for NPC metastasis and prognosis. PMID: 30151927
- The presence of varicocele has been linked to lower serum inhibin B levels. PMID: 27423503
- Elevated activin B levels, alongside normal activin A levels, have been identified in patients exhibiting the diagnostic symptoms of chronic fatigue syndrome/myalgic encephalomyelitis. PMID: 28302133
- Research has identified Inhbb as a key factor mediating the proliferative effect of Pdx-1 on islet cells. PMID: 27620967
- Low activin B levels have been associated with tubal ectopic pregnancy. PMID: 26968108
- Pregnant Han Chinese women carrying the rs7579169 CC genotype and G-G-C haplotype have a significantly higher likelihood of developing pre-eclampsia, particularly late-onset and multiparous cases. PMID: 26313529
- Observations have shown that activin-B is upregulated in the lungs of individuals with idiopathic pulmonary fibrosis. PMID: 25361680
- A reduction in RhoA signaling by Activin B, coupled with persistent Rac1 activity, is a prerequisite for inducing an invasive phenotype in clear cell renal cell carcinoma. PMID: 25343250
- Serum concentrations of inhibin B and anti-Mullerian hormone have not proven to be valuable as an additional diagnostic tool for male infertility. PMID: 25581422
- Inhibin B levels, but not FSH, are elevated in primary hypothyroidism, consistent with a hypogonadotrophic hypogonadal state affecting the reproductive segment of the gonadotrophic axis. PMID: 23692058
- Significant differences in serum inhibin B levels have been observed in patients with diminished ovarian reserve before and after supplementation with dehydroepiandrosterone (DHEA). PMID: 23664458
- Inhibin B production was decreased following administration of recombinant human chorionic gonadotropin in both control and polycystic ovary syndrome women. PMID: 24188875
- Inhibin B was measured in plasma samples obtained from 34 healthy male subjects selected based on criteria typical for a phase I clinical trial across a wide age range. PMID: 23349064
- Data suggest that neither serum inhibin B nor follicle-stimulating hormone (FSH) are suitable surrogates for determining sperm concentration in a semen sample. PMID: 23423746
- While the signaling pathway responsible for hepcidin upregulation in inflammatory contexts is not fully understood, activin B has been shown to play a crucial, albeit unexpected, role in the induction of hepcidin by inflammation. PMID: 22611157
- A genome-wide association study for preeclampsia in unrelated Australian individuals of Caucasian ancestry was conducted to genotype single nucleotide polymorphisms in the Inhibin, beta B gene on 2q14.2, in preeclampsia and normal pregnancy controls. PMID: 22432041
- The inhibin-betaB subunit does not appear to be a useful prognostic marker for endometrioid adenocarcinomas. PMID: 21938679
- Compared to antimullerian hormone (AMH), stimulated serum inhBB is a more accurate predictor of ovarian response in patients undergoing in vitro fertilization. PMID: 21843890
- Inhibin B serves as a reliable marker of Sertoli cell function and spermatogenesis, while the clinical significance of anti-Mullerian hormone in men with varicocele remains to be fully elucidated. PMID: 21285453
- High inhibin beta B levels are associated with cervical intraepithelial neoplasia grades 1 and 2 compared to cervix uteri. PMID: 21475087
- Inhibin B and antimullerian hormone play a role in gonadal function following treatment for medulloblastoma or posterior fossa ependymoma during childhood. PMID: 21168856
- A variant in the fibrillin-3 gene has been associated with TGF-beta and inhibin B levels in women with polycystic ovary syndrome. PMID: 20630504
- This study investigates the effect of obesity on testicular function by evaluating reproductive hormones, inhibin B, insulin-like 3 (INSL3), and leptin, in obese and non-obese adolescents according to pubertal Tanner stages. PMID: 20713036
- Serum inhibin-b levels decrease nonlinearly during the daytime and are positively correlated with sperm counts. However, the predictive power is strongest when inhibin-b levels are low. PMID: 20149358
- Antral follicle size influences serum inhibin B and FSH levels and alters their expected relationship with the number of antral follicles on day 3. PMID: 19061996
- Gonads in individuals with anorexia nervosa exhibit low levels of activity upon weight gain. Inhibin B serves as an early marker of gonadal activity, and the awakening of reproductive function is gradual with weight gain. PMID: 15070953
- The inhibin B pubertal surge is a prominent signal of gonadal maturation in both females and males. Significantly higher levels are observed in boys compared to girls. This is a review of the topic. PMID: 15319819
- The expression of inhibin betaB by Sertoli cells is dependent on the presence of spermatogenic activity within these seminiferous tubules. Consequently, it is low in patients with Sertoli cell only syndrome. PMID: 15551748
- Ovulatory cycles are characterized by higher FSH and lower inhibin B levels in cases of hypergonadotropic hypogonadism or premature ovarian failure. PMID: 15562017
- Inhibin B (InhB) exhibits a regular increase during puberty. In the early stages of gonadal maturation, InhB and FSH correlate positively, while this relationship becomes inverse in the mid to late stages. Serum InhB increases during mid-puberty (G3-G4). PMID: 15757857
- Exposure of Sertoli cells to excessive amounts of lead results in inappropriate inhibin B overproduction, which may contribute to the impairment of spermatogenesis. PMID: 15910540
- Inhibin B may play a role in male fecundity. PMID: 16024538
- INHBB expression was found to be high in human adipocytes, reduced by weight loss, and adipose tissue INHBB mRNA levels correlated with metabolic risk factors. PMID: 16650820
- A significant downregulation of the inhibin-beta (B) subunit was observed in extravillous trophoblast cells in IUGR syncytiotrophoblast cells. PMID: 16670820
- Preoperative serum inhibin B concentration did not reliably predict a response to varicocelectomy. However, an increase in inhibin B levels after treatment might suggest an improvement in testicular function. PMID: 16728349
- Follistatin was overexpressed, while both activin subunits were downregulated in the majority of rat and human liver tumors. PMID: 16935389
- Treatment with vaginal estroprogestinic has been shown to decrease serum inhibin A and inhibin B levels, as well as follicular diameter. PMID: 16989826
- Varicocele sclerotherapy has been observed to improve inhibin B levels and seminal parameters. PMID: 17376219
- The exact role of inhibin B in the mechanism of action of laparoscopic ovarian diathermy remains unclear. PMID: 17525068
- Granulosa cell production of inhibin B is reduced by pharmacologically induced increases in intrafollicular androgen levels. PMID: 17628551
- Decreased levels of inhibin B have been associated with hot flushes, aches, joint pain, stiffness, and depressed mood during the stages of menopausal transition. PMID: 17666595
- Activin B is a potent inducer of Pdx1, as well as Shh, in differentiating embryonic stem cell-derived embryoid bodies. PMID: 17761145
- Activin and estrogen engage in crosstalk that regulates transcription in human breast cancer cells. PMID: 17914098
- A monoclonal antibody targeting xenopus laevis Inhbb has been utilized in ELISA for the detection of human Inhbb. PMID: 17991484
- The expression of the beta-B subunit is associated with decidualization and can be detected in the circulation as activin B. PMID: 18381568
- The degree of inhibin B increment during controlled ovarian stimulation provides a means for predicting ovarian response to ovarian stimulation. PMID: 18555227
- No significant association was found between (log)inhibin B profiles and age at final menstrual period. PMID: 18593767
- Functional validation of activins and bone morphogenetic protein 11 as candidate novel muscle mass regulators has been performed. PMID: 18927237
- Serum inhibin B and estradiol concentrations obtained shortly after Gn therapy may offer an accurate and early prediction of ovarian response. PMID: 19198021
- Inhibin B is more potent than inhibin A in suppressing FSH both in vitro and in vivo. This difference in bioactivity cannot be solely explained by its affinity to betaglycan or activin type II receptors. PMID: 19589860
