INTS4 Antibody
Description
Antibody Characteristics
INTS4 antibodies are polyclonal reagents typically raised in rabbits, targeting human, mouse, and rat INTS4 proteins. Key features include:
| Property | Details |
|---|---|
| Host Species | Rabbit (IgG) |
| Reactivity | Human, Mouse, Rat |
| Applications | Western Blot (WB), ELISA, Immunoprecipitation (IP) |
| Molecular Weight | 108 kDa (observed); 963 amino acids (calculated) |
| Immunogen | Recombinant fusion protein or peptide sequences (e.g., amino acids 1–270 or 792–841 of INTS4) |
| Storage | -20°C in PBS with 0.02% sodium azide and 50% glycerol |
Biological Role of INTS4
INTS4 is a scaffold protein within the integrator complex, a ~1 MDa assembly associated with RNA polymerase II. Key functions include:
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snRNA Processing: Mediates 3′-end cleavage of small nuclear RNAs (snRNAs) such as U1 and U2, enabling their maturation .
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Cajal Body Integrity: Maintains structural cohesion of Cajal bodies, nuclear organelles critical for snRNP biogenesis. Depletion disrupts coilin and SMN localization, leading to nucleolar accumulation of coilin and cytoplasmic SMN granules .
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Transcriptional Regulation: Facilitates RNAPII elongation and nucleosome positioning at genes involved in transcriptional repression and growth signaling .
Functional Insights
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Knockdown Effects: siRNA-mediated INTS4 depletion reduces snRNA processing efficiency by >50%, disrupts Cajal body integrity, and causes mislocalization of Sm proteins and snRNAs .
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Structural Role: INTS4 stabilizes the INTS9/11 heterodimer (analogous to CPSF73/100 in the cleavage and polyadenylation complex) via N-terminal HEAT repeats and a C-terminal β-sheet domain .
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Complex Assembly: INTS4 is essential for integrator complex formation. Its absence redistributes INTS11 to lower molecular weight fractions, abolishing enzymatic activity .
Disease Relevance
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Cancer: Dysregulation of INTS4-linked pathways (e.g., EGFR signaling) correlates with tumorigenesis .
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Neurodegeneration: Defective Cajal body function due to INTS4 depletion mirrors pathologies observed in spinal muscular atrophy .
Comparative Antibody Performance
Technical Considerations
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Cross-Reactivity: Confirmed in human, mouse, rat, and Xenopus .
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Buffer Compatibility: Compatible with standard SDS-PAGE and RIPA lysis buffers. Avoid repeated freeze-thaw cycles .
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Controls: Use HeLa or MCF-7 cell lysates for WB validation; nuclear fractions for IP .
Future Directions
Current research focuses on:
Product Specs
Target Background
- Research findings indicate that the Integrator complex is essential for snRNA maturation and Cajal body homeostasis. PMID: 22250197
