INTS8 Antibody
Shipped with Ice Packs 
In Stock 
Description
Experimental Use Cases
The INTS8 antibody has been validated in multiple studies:
Recommended Dilutions
| Application | Dilution Range |
|---|---|
| Western Blot (WB) | 1:200–1:1000 |
| Immunoprecipitation (IP) | 0.5–4.0 µg per 1–3 mg lysate |
| Immunofluorescence (IF) | 1:10–1:100 |
Technical Notes
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Optimization: Titration is critical due to batch-specific variability .
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Controls: Use HIBE (normal biliary epithelial) and CHOL cell lines (HCCC-9810, RBE) for comparative studies .
Functional Roles
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Transcriptional Regulation: INTS8 is a core component of the Integrator complex, essential for snRNA 3'-end processing and transcriptional termination .
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Cancer Progression: Drives epithelial-to-mesenchymal transition (EMT) via TGF-β signaling in HCC and CHOL .
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Neurodevelopmental Disorders: Mutations in INTS8 destabilize the Integrator complex, impairing neuronal differentiation and mRNA processing .
Clinical Implications
Product Specs
Buffer
PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. Store at -20°C. Avoid freeze/thaw cycles.
Lead Time
Typically, we can ship your order within 1-3 business days of receipt. Delivery times may vary depending on the purchase method and location. Please contact your local distributor for specific delivery timelines.
Synonyms
INTS8 antibody; C8orf52 antibody; Integrator complex subunit 8 antibody; Int8 antibody; Protein kaonashi-1 antibody
Target Names
INTS8
Uniprot No.
Target Background
Function
INTS8 is a component of the Integrator complex. This complex is involved in the transcription of small nuclear RNAs (snRNAs) U1 and U2, and their 3'-box-dependent processing. The Integrator complex associates with the C-terminal domain (CTD) of the RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNA genes.
Gene References Into Functions
- Mutations in INTS8 have been linked to a severe neurodevelopmental syndrome. These mutations lead to instability of the Integrator complex and impaired function in vitro. PMID: 28542170
Database Links
Protein Families
Integrator subunit 8 family
Subcellular Location
Nucleus.
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