IST1 Antibody, Biotin conjugated
Description
IST1 Antibody Overview
IST1 is an ESCRT-III-associated protein critical for cytokinesis and membrane remodeling. Antibodies targeting IST1 are polyclonal or monoclonal, with reactivity across species (human, mouse, rat) and applications in Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF) .
Key Features of IST1 Antibodies
Biotin Conjugation: Purpose and Mechanism
Biotinylated antibodies leverage high-affinity binding between biotin and streptavidin/avidin. This conjugation enables:
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Signal amplification via streptavidin-linked enzymes (e.g., HRP, AP) in ELISA or WB.
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Flexibility in detection methods (e.g., switching between fluorophores or beads).
General Workflow for Biotin-Conjugated Antibodies
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Primary Antibody: Biotinylated IST1 antibody binds target protein.
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Secondary Detection: Streptavidin-conjugated reagent (HRP, fluorophore) amplifies signal.
Potential Applications for Biotin-Conjugated IST1 Antibodies
While no commercial biotin-conjugated IST1 antibodies are explicitly listed in sources, extrapolating from existing IST1 antibody data and biotin conjugation principles reveals potential uses:
Enhanced Sensitivity in Western Blotting
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Example: A biotinylated IST1 antibody (e.g., 1:5000 dilution) paired with streptavidin-HRP could improve detection of low-abundance IST1 in cell lysates (e.g., Raji cells) .
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Data: Unconjugated IST1 antibodies detect ~36–40 kDa bands in WB . Biotin conjugation may reduce background noise.
Multiplexed Immunofluorescence
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Use Case: Co-staining IST1 (biotin) with other ESCRT components (e.g., VPS4A) using fluorophore-linked streptavidin.
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Support: Proteintech’s IST1 antibody (51002-1-AP) is validated for IF .
Affinity Purification
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Method: Biotinylated IST1 antibody bound to streptavidin beads could isolate IST1 complexes for mass spectrometry.
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Relevance: IST1 interacts with calpain 7 and ESCRT machinery .
Current Limitations and Challenges
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Commercial Availability: No biotin-conjugated IST1 antibodies are explicitly listed in sources. Existing IST1 antibodies are unconjugated .
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Custom Synthesis: Researchers may need to biotinylate IST1 antibodies post-purchase using NHS-biotin kits.
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Validation Gaps: Biotin conjugation may alter antibody specificity or affinity, necessitating re-validation.
IST1 Antibody Specificity and Reactivity
Biotin Conjugation in Related Antibodies
| Application | Typical Biotin Use | Detection Method | Example |
|---|---|---|---|
| ELISA | Biotinylated primary + streptavidin-HRP | Colorimetric (TMB substrate) | High-throughput screening |
| IHC | Biotinylated primary + streptavidin-fluorophore | Fluorescence microscopy | Co-localization studies |
| Affinity Purification | Biotinylated primary + streptavidin beads | Mass spectrometry | Protein complex analysis |
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Overexpression of OLC1 in gastric adenocarcinoma was linked to clinicopathological parameters and predicted poor overall survival. PMID: 28038462
- The expression of OLC1 was found to be higher in the tumor tissues of lung adenocarcinoma compared to squamous cell carcinoma. PMID: 28532543
- Structural and biochemical studies revealed an unusually tight interaction between ULK3 and IST1, an ESCRT-III subunit required for cytokinetic abscission. PMID: 26011858
- Overexpression of OLC1 was found to promote tumorigenesis of human esophageal squamous cell carcinoma. PMID: 24608342
- Findings suggest that a high expression level of OLC1 serves as a biomarker for poor prognosis in breast cancer, indicating that OLC1 may be a potential target for antiangiogenic therapy. PMID: 24880589
- Over-expression of OCL1 has been associated with colorectal cancer. PMID: 24403489
- The binding of ALG-2 to IST1 is Ca(2+)-dependent. PMID: 23649269
- Research suggests that the inclusion of IST1 into the ESCRT complex allows for the recruitment of spastin, promoting fission of recycling tubules from the endosome. PMID: 23897888
- OLC1 over-expression is a significant factor in epithelial ovarian carcinoma prognosis and may serve as a potential biomarker for ovarian carcinoma. PMID: 23609236
- The interaction between CL7MIT and CHMP1B, as well as CL7MIT and IST1, became stronger when IST1 or CHMP1B was additionally coexpressed, indicating the formation of a ternary complex of calpain-7, IST1, and CHMP1B. PMID: 21616915
- Studies demonstrate that cigarette smoke regulates OLC1 expression in lung cancer cells by compromising its ubiquitination and subsequent degradation through the ubiquitin E3 ligase APC. PMID: 21439932
- Data indicates that Ist1 interaction is vital for spartin recruitment to the midbody, suggesting that spartin participates in cytokinesis. PMID: 20719964
- Results demonstrate that human calpain 7 is proteolytically active, implying that calpain 7 is activated by ESCRT-III-related protein IST1. PMID: 20849418
- OLC1 (KIAA0174) is a candidate oncogene in lung cancer whose expression may be regulated by exposure to cigarette smoke. PMID: 19001599
- IST1 and CHMP1 act together to recruit and modulate specific VPS4 activities necessary during the final stages of cell division. PMID: 19129479
- hIST1 is essential for cytokinesis in mammalian cells. PMID: 19129480
- Data reveals that the N-terminal core domains of increased sodium tolerance-1 (IST1) and charged multivesicular body protein-3 (CHMP3) form equivalent four-helix bundles, highlighting that IST1 is a previously unrecognized ESCRT-III family member. PMID: 19525971
