ITGB5 Antibody, FITC conjugated
Description
Definition and Structure
ITGB5 is a transmembrane glycoprotein that pairs with the αV subunit to form the αVβ5 integrin heterodimer. This complex binds ligands like vitronectin and fibronectin via the RGD motif, mediating cell-matrix interactions . The FITC-conjugated ITGB5 antibody enables fluorescent detection of this protein in live or fixed samples.
Applications and Validation
ITGB5 FITC-conjugated antibodies are validated for diverse techniques:
Validation Metrics:
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Specificity: Confirmed via knockdown/overexpression studies .
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Cross-Reactivity: Human and mouse ; predicted reactivity with rat .
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Citations: Peer-reviewed studies (e.g., 29 publications for ab15459) .
Role in Cancer Metastasis:
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TSCC: High ITGB5 expression correlates with lymph node metastasis and poor prognosis. ROS-induced ITGB5 upregulation promotes EMT and metastasis via cell adhesion pathways .
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Breast Cancer: ITGB5 drives migration, proliferation, and tumor angiogenesis through Src-FAK and MEK-ERK signaling .
Functional Mechanisms:
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Cell Adhesion: Mediates binding to vitronectin and fibronectin .
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Signaling: Activates FAK (Tyr861), Paxillin (Tyr31), and ERK1/2 pathways .
Limitations and Considerations
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Cross-Reactivity: Potential off-target binding in murine models .
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Sample Compatibility: Performance varies by cell type (e.g., ineffective in lymphoblastoid cells) .
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Storage Stability: FITC fluorescence degrades with light exposure; requires dark storage .
Key Citations
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Elevated ITGB5 expression has been correlated with cervical cancer. PMID: 26758421
- FAK activity regulates beta5 integrin expression and anchorage-independent cell growth in ovarian carcinoma. PMID: 24899686
- FRMD5 regulates tumor cell motility through a dual pathway involving FRMD5 binding to integrin beta5 tail and to ROCK1. PMID: 25448675
- Immunohistochemical analysis of alphavbeta3, alphavbeta5, and alphavbeta6 integrin subunits has been performed and correlated with Ki67 and hypoxia-inducible factor (HIF)-1alpha indexes. PMID: 25150423
- Myo7a interacts with integrin beta5 and specifically promotes integrin alphavbeta5-mediated cell migration. PMID: 24997346
- Antibody blocking experiments have demonstrated that aV b5 and a6 significantly promote hESC attachment in 2% oxygen only, while blockage of CD44 inhibits cell attachment in 21% O2 alone. PMID: 24455718
- Brain metastases ITGB5 expression exhibits significant heterogeneity based on tumor origin. PMID: 24294359
- Integrin-beta5 and zyxin mediate the formation of ventral stress fibers in response to transforming growth factor beta. PMID: 24036928
- A molecular model of integrin b5 structure was prepared and its stereochemical model quality was verified. Integrin b5 active sites were identified using in silico analysis tools. PMID: 23962022
- BDNF enhances chondrosarcoma cell migration by increasing beta5 integrin expression through a signal transduction pathway involving the TrkB receptor, PI3K, Akt, and NF-kappaB. PMID: 23874483
- The top primary GWAS association of Airway hyperresponsiveness was found in rs848788 (P-value 7.2E-07) located within the ITGB5 genes. PMID: 23984888
- Depletion of integrin beta5 in triple-negative breast carcinoma cells significantly reduced tumor take, growth, and tumor angiogenesis. Reexpression of integrin beta5 rescued this phenotype, indicating a crucial role for integrin beta5 in the tumorigenic potential of breast carcinoma cells. PMID: 22824793
- High beta5-integrin protein expression has been associated with aggressive behavior in gastric cancer. PMID: 22561002
- Loss of integrin beta5 in polyomavirus positive Merkel cell carcinoma may influence cell adhesion/migration. PMID: 22503669
- Cysteine-rich protein Cyr61 activates interleukin (IL)-6 production via the alphavbeta5/Akt/NF-kappaB signaling pathway in rheumatoid arthritis. PMID: 22547695
- The beta5-integrin adhesions contribute to the TGFbeta-induced EMT and the tumorigenic potential of carcinoma cells. PMID: 20404485
- p21-activated kinase 4 phosphorylation of integrin beta5 Ser-759 and Ser-762 regulates cell migration. PMID: 20507994
- Src kinase-mediated activation of STAT3 and subsequent angiogenic gene expression mediate the effects of integrin alpha v beta 5 and may be exploited to enhance the paracrine activities of circulating angiogenic cells. PMID: 20431064
- Overexpression of the beta5 integrin in hematopoietic cells has been associated with the inhibition of cell proliferation and apoptosis. PMID: 11877043
- There are different beta5 integrins: repeated-FNK (FNKFNK764-769) and single-FNK (FNK764-766) amino acid sequences in the cytoplasmic domain. PMID: 15979906
- Studies suggest that the phagocytic function of beta5 integrin is regulated by an unconventional NPxY-talin-independent activation signal and argue for the existence of molecular switches in the beta5 cytoplasmic tail for adhesion and phagocytosis. PMID: 17963729
