ITGB6 Antibody, FITC conjugated
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Description
Applications and Recommended Dilutions
This antibody is validated for multiple experimental workflows:
Role in Cancer and Therapeutic Resistance
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Anti-CD276 Therapy Modulation: ITGB6 knockdown (cKO) synergizes with α-CD276 treatment, significantly reducing tumor size in HNSCC models. ITGB6 ablation enhances CD8+ T-cell infiltration and caspase-3 activation in tumor cells, suggesting immune-mediated cytotoxicity .
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Antibody–Drug Conjugate (ADC) Development: ITGB6-targeted ADCs (e.g., SGN-B6A with MMAE payload) show efficacy in colorectal, lung, and gastric cancers. Preclinical studies report tumor regression with minimal toxicity due to ITGB6’s restricted expression in normal tissues .
Mechanistic Insights
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Ligand Binding: Recognizes R-G-D motifs in fibronectin, cytotactin, and fibrillin-1, facilitating cell-ECM interactions .
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TGF-β Activation: Mediates release of active TGF-β1 from latency-associated peptide (LAP), promoting immunosuppression and metastasis .
Technical Considerations
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Cross-Reactivity: No observed binding to other αv integrins (e.g., αvβ3, αvβ5) .
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Storage Stability: Stable for 24 months at -20°C; avoid freeze-thaw cycles .
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Controls: Recommended to use ITGB6-transfected cell lines (e.g., SW480 ITGB6-high) for validation .
Emerging Clinical Relevance
Product Specs
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
We typically dispatch products within 1-3 business days of receiving your order. Delivery times may vary based on the mode of purchase and destination. For specific delivery details, please consult your local distributor.
Synonyms
ITGB6; Integrin beta-6
Target Names
Uniprot No.
Target Background
Function
Integrin alpha-V:beta-6 (ITGAV:ITGB6) is a receptor for fibronectin and cytotactin, recognizing the R-G-D sequence in its ligands. Internalization of integrin alpha-V/beta-6 via clathrin-mediated endocytosis facilitates carcinoma cell invasion. ITGAV:ITGB6 acts as a receptor for fibrillin-1 (FBN1), mediating R-G-D-dependent cell adhesion to FBN1. Integrin alpha-V:beta-6 (ITGAV:ITGB6) plays a crucial role in TGF-beta-1 activation by mediating R-G-D-dependent release of transforming growth factor beta-1 (TGF-beta-1) from regulatory Latency-associated peptide (LAP).
Regarding microbial infections, integrin ITGAV:ITGB6 acts as a receptor for Coxsackievirus A9 and Coxsackievirus B1, as well as Herpes simplex virus-1/HHV-1.
Regarding microbial infections, integrin ITGAV:ITGB6 acts as a receptor for Coxsackievirus A9 and Coxsackievirus B1, as well as Herpes simplex virus-1/HHV-1.
Gene References Into Functions
- These findings highlight JunB and CBP-mediated histone hyperacetylation as the driving force behind TGF-beta1 induced ITGB6 transcription in oral squamous cell carcinoma (OSCC) cells. This suggests that epigenetic mechanisms are responsible for the active transcriptional expression of ITGB6 induced by TGF-beta1 in OSCC cells. PMID: 29274289
- Our results demonstrate that integrin beta6 promotes tumor invasiveness in a Rac1-dependent manner, making it a potential biomarker for tumor metastasis. PMID: 27440504
- The increased regional and distant metastasis observed in HER2+ tumors with high levels of ITGB6 could be mediated by the canonical Rho-Rac pathway through increased expression of MMP9 and MMP15. PMID: 27184932
- The rare missense variant c.898G>A (p.(Glu300Lys)) in ITGB6 is associated with not only dentogingival anomalies but also intellectual disability and alopecia. PMID: 26695873
- Treatment of these cells with the dual-specificity tyrosine-kinase inhibitor lapatinib resulted in downregulation of epithelial-to-mesenchymal transition, as evidenced by lower levels of SNAI1 and SNAI2 transcripts, integrin AVB6, and matrix metalloproteinase 9 protein. PMID: 28349782
- Integrin b6 significantly promoted the proliferation and invasion of pancreatic carcinoma cells, inducing ETS1 phosphorylation in an ERK-dependent manner. This led to the upregulation of matrix metalloprotease-9, which is essential for b6-mediated invasiveness of pancreatic carcinoma cells. PMID: 26547582
- AG/GG genotypes of ITGB6 rs4665162 gene were associated with a higher risk of radiation pneumonitis (RP) in patients with lung cancer receiving radiotherapy, suggesting its potential as a reliable predictor of RP. PMID: 26449830
- A novel homozygous ITGB6 mutation causing isolated amelogenesis imperfecta has been identified, contributing to a deeper understanding of normal and pathologic enamel development. PMID: 25431241
- These data reveal a novel role for Elk1 in regulating ITGB6 expression, highlighting how dysregulation of Elk1 can contribute to human disease. PMID: 26861876
- Integrin beta6 expression correlated significantly with MMP-9 expression and may serve as a valuable recurrence indicator for follicular thyroid carcinomas. PMID: 24844802
- Results indicate that MMP-9-degraded Fibronectin can adjust constituents of the avb6 heterodimers in the early phase and significantly elevate amounts of b6 integrin subunits later on. PMID: 26134759
- The ITGB6 promoter contains a functional TATA box, with STAT3 and C/EBPalpha involved in its positive regulation in oral squamous cell carcinoma cells. PMID: 25816241
- Targeting eIF4E in integrin ava6 expressing tumors could be a potential therapeutic strategy for patients with colon cancer. PMID: 25982998
- NSCLC cell lines, positive for E-Cadherin, EpCAM, and avb6 expression, activate normal fibroblasts through avbeta6/TGFbeta signaling in vitro, influencing both gene expression and response to therapeutic agents. PMID: 24488011
- The deficiency of avb6-related hyperpermeability in T84 monolayers can be compensated by adding exogenous avb6 to the culture. PMID: 24677750
- Expression of the alphavbeta6 integrin subunit appears to be of pathobiological and clinical relevance in patients with non-small cell lung cancer with brain metastases. PMID: 25150423
- ITGB6 loss-of-function mutations cause autosomal recessive amelogenesis imperfecta. PMID: 24305999
- Brain metastases ITGB6 expression exhibits considerable heterogeneity according to tumor origin. PMID: 24294359
- ADAM 10 is overexpressed in oral squamous cell carcinoma and contributes to invasive behavior through a functional association with alphavbeta6 integrin. PMID: 24055471
- Data indicate that integrin beta6, CD46, tissue factor, and chromosome 14 open reading frame 1 (C14ORF1) were identified as overexpressed on pancreatic cancer cell lines. PMID: 21934552
- These data identify a novel interaction between Psor and beta6, demonstrating that it is required for alphavbeta6-dependent invasion by carcinoma cells. PMID: 21132011
- Integrin beta6 plays a role in the compromised wound healing associated with the diabetic state. PMID: 20854469
- Fusion of epithelial cells by Epstein-Barr virus proteins is triggered by the binding of viral glycoproteins gHgL to integrins alphavbeta6 or alphavbeta8. PMID: 19920174
- Integrin expression in colon cancer cells is regulated by the cytoplasmic domain of the beta6 integrin subunit. PMID: 11992542
- Loss of integrin alpha(v)beta6-mediated TGF-beta activation causes Mmp12-dependent emphysema. PMID: 12634787
- Upon ligation of integrin beta6 with fibronectin, beta6 complexed with Fyn and activated it, activating a pathway leading to activation of the matrix metalloproteinase-3 gene, and promoting oral SCC cell proliferation and experimental metastasis in vivo. PMID: 12917446
- beta(6) integrin is not normally expressed in adult native or transplanted kidneys but is commonly up-regulated in the distal tubule in disease. PMID: 15458435
- Constitutively activated STAT3 induces tumorigenesis and enhances cell motility of prostate epithelial cells through ITGB6. PMID: 17438134
Database Links
Involvement In Disease
Amelogenesis imperfecta 1H (AI1H)
Protein Families
Integrin beta chain family
Subcellular Location
Membrane; Single-pass type I membrane protein. Cell junction, focal adhesion.
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