ITIH1 Antibody, HRP conjugated
Description
Composition and Mechanism
ITIH1 Antibody, HRP conjugated consists of a polyclonal antibody specific to human ITIH1, chemically linked to HRP. The antibody targets epitopes within amino acids 403–638 of the ITIH1 protein . Its mechanism involves:
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Antigen capture: Pre-coated primary antibodies immobilize ITIH1 in microplate wells.
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Signal amplification: HRP catalyzes a colorimetric reaction using TMB substrate, producing measurable optical density proportional to ITIH1 concentration .
Cancer Biology
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Tumor Suppression Studies: ITIH1 knockdown in renal cell carcinoma (RCC) cells increased proliferation (p<0.05) and invasion, while overexpression suppressed these effects .
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Pathway Analysis: ITIH1 modulates NF-κB signaling; inhibition with JSH-23 reversed pro-tumorigenic effects in RCC .
Biomarker Quantification
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Clinical Samples: Validated for use in serum, plasma, and tissue homogenates with recovery rates of 85–105% .
Validation and Quality Control
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Specificity: Demonstrated high specificity for human ITIH1, though cross-reactivity with homologs remains incompletely characterized .
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Precision: Intra- and inter-assay coefficients of variation (CV) ≤10% ensure reproducibility .
Limitations and Considerations
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Thrombin treatment of the inflammatory matrix leads to the dissolution of HC-HA cable structures and inhibits leukocyte adhesion. These findings establish a novel mechanism whereby thrombin cleavage of HC1 regulates the adhesive properties of an inflammatory HA matrix. PMID: 27679489
- Data suggest that Mg2+ or Mn2+ (but not Ca2+) induce a conformational change in inter-alpha-inhibitor (ITIH1 and ITIH2) and a bikunin/chondroitin sulfate-dependent increase in thermodynamic stability. Notably, bikunin binds adjacent to the two heavy chains. PMID: 26728454
- Our study revealed that shared genetic risk factors for both schizophrenia and major depressive disorder exist in ITIH family genes within the Han Chinese population. PMID: 24389398
- Data indicate that TSG-6-mediated cross-linking of hyaluronan (HA) films is impaired in the presence of inter-alpha-inhibitor (IalphaI) and that this effect suppresses the TSG-6-mediated enhancement of HA binding to CD44-positive cells. PMID: 24005673
- Human inter-alpha-inhibitor serves as a substrate for factor XIIIa and tissue transglutaminase. PMID: 21939789
- Transfection into H460M Human large cell lung carcinoma line resulted in a decrease in tumor metastasis in nude mice. PMID: 11807786
- PTX3 directly interacts with inter-alpha-trypsin inhibitor and plays a role in hyaluronan organization and cumulus oophorus expansion. PMID: 17675295
- The first systematic analysis on the differential expression of ITIH genes in human cancer, demonstrating frequent downregulation which may be associated with the initiation and/or progression of these malignancies. PMID: 18226209
