JAM2 antibody
Description
Introduction to JAM2 Antibody
JAM2 (Junctional Adhesion Molecule 2) is a member of the immunoglobulin superfamily (IgSF) that mediates heterotypic cell-cell interactions via its receptor JAM3. It is expressed in endothelial cells, lymphatic vessels, and hematopoietic stem cell niches, playing roles in leukocyte transmigration, spermatogenesis, and tissue homeostasis . JAM2 antibodies are immunological reagents designed to detect and modulate JAM2 activity in experimental and clinical settings. These antibodies are widely used to investigate JAM2's involvement in cancer biology, immune responses, and vascular function.
Key Applications of JAM2 Antibodies
JAM2 antibodies are validated for diverse applications:
Role in Cancer Biology
-
Downregulation in Lung Adenocarcinoma (LUAD): JAM2 is suppressed in LUAD tissues and cell lines (A549, H1299) due to promoter hypermethylation. Overexpression inhibits epithelial-mesenchymal transition (EMT) and reduces metastatic potential .
-
Immune Infiltration: Higher JAM2 expression correlates with increased tumor-infiltrating lymphocytes (e.g., CD8+ T cells, NK cells), suggesting prognostic value for immunotherapy response .
Immune Regulation
-
Leukocyte Extravasation: Anti-JAM2 antibodies block leukocyte migration across endothelial monolayers by disrupting JAM2-integrin α4β1 interactions .
-
Lymphocyte Homing: JAM2 guides lymphocyte trafficking to lymphoid organs via HEV interactions, a process inhibitable by soluble JAM2 .
Other Biological Functions
Product Specs
Junctional Adhesion Molecule 2 (JAM2) belongs to the immunoglobulin superfamily and the junctional adhesion molecule (JAM) family. As a type I membrane protein, it resides at the tight junctions of both epithelial and endothelial cells. JAM2 acts as an adhesive ligand, facilitating interactions with various immune cells. It is believed to play a role in guiding lymphocytes to secondary lymphoid organs.
The solution has a concentration of 1mg/ml and contains PBS (pH 7.4), 10% glycerol, and 0.02% sodium azide.
The specificity and reactivity of the JAM2 antibody have been validated using ELISA and Western blot analysis. However, it is recommended to optimize the reagent concentration for each specific application to achieve the best results.
Junctional adhesion molecule B, JAM-B, Junctional adhesion molecule 2, JAM-2, Vascular endothelial junction-associated molecule, VE-JAM, CD322.
JAM2 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
PAT21D7AT.
Anti-human JAM2 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human JAM2 protein 21-238 amino acids purified from E. coli.
Mouse IgG2a heavy chain and k light chain.
Product Science Overview
Junctional Adhesion Molecule 2 (JAM-2), also known as JAM-B, is a member of the immunoglobulin superfamily. It plays a crucial role in the regulation of cell adhesion, migration, and permeability of endothelial and epithelial cells. JAM-2 is prominently expressed on high endothelial venules (HEVs) and is involved in the transmigration of lymphocytes across the endothelium .
JAM-2 is a type I transmembrane protein with two extracellular immunoglobulin-like domains, a single transmembrane region, and a short cytoplasmic tail. It is expressed on the surface of endothelial cells, particularly in HEVs within lymph nodes and Peyer’s patches . Additionally, JAM-2 is found on a subset of leukocytes and is involved in the regulation of immune cell trafficking .
JAM-2 plays a pivotal role in the immune system by facilitating the transmigration of lymphocytes across the endothelial barrier. This process, known as extravasation, is essential for immune surveillance and the body’s defense against pathogens . JAM-2 interacts with other adhesion molecules and signaling proteins to regulate cell migration and maintain the integrity of the endothelial barrier .
The mouse anti-human JAM-2 antibody is a monoclonal antibody derived from hybridization of mouse myeloma cells with spleen cells from immunized mice. This antibody specifically binds to human JAM-2 and is used in various research applications, including flow cytometry, immunohistochemistry, and Western blotting .
The mouse anti-human JAM-2 antibody is widely used in research to study the role of JAM-2 in cell adhesion, migration, and immune cell trafficking. It is also employed in the investigation of JAM-2’s involvement in various pathological conditions, such as inflammation, cancer, and cardiovascular diseases .
