JUN (Ab-91) Antibody
Product Specs
Target Background
- Data indicated that miR-139-5p was down-regulated in the hearts of Hypertrophic cardiomyopathy patients and that it inhibited cardiac hypertrophy by targeting c-Jun expression. PMID: 29440459
- This study identified an essential Jun/miR-22/HuR regulatory axis in CRC (the working model is summarized in Fig. 8) and highlighted the vital role of HuR and miR-22 in CRC proliferation and migration. PMID: 29351796
- A novel cascade mediated by AP-1 and FOXF1 that regulates oncogene-induced senescence is reported. PMID: 30119690
- Multivalent Interactions with Fbw7 and Pin1 Facilitate Recognition of c-Jun by the Fbw7. PMID: 29225075
- High AP-1 expression is associated with metastasis in colon cancer. PMID: 29305742
- Our results suggest that extended AP-1 binding sites, together with adjacent binding sites for additional TFs, encode part of the information that governs transcription factor binding sites activity in the genome. PMID: 29305491
- The expression of WIF-1 was low in GBC cells due to aberrant hypermethylation of its promoter region. Additionally, an alternative pathogenesis of GBC was indicated in which c-Jun causes hypermethylation of the WIF-1 promoter region, and represses the expression of WIF-1 through transcriptional regulation and interaction with DNMT1 as an early event in the tumorigenesis of GBC. PMID: 29693707
- Mutant cellular AP-1 proteins promote expression of a subset of Epstein-Barr virus late genes in the absence of lytic viral DNA replication. PMID: 30021895
- Secreted Ta9 has therefore, not only the ability to stimulate CD8+ T cells, but also the potential to activate AP-1-driven transcription and contribute to T. annulata-induced leukocyte transformation PMID: 29738531
- MiR-216b directly targets c-Jun, thereby reducing AP-1-dependent transcription and sensitizing cells to ER stress-dependent apoptosis. PMID: 27173017
- Results suggest that c-Jun, p38 MAPK, PIK3CA/Akt, and GSK3 signaling are involved in the effect of miR-203 on the proliferation of hepatocellular carcinoma cells. PMID: 28887744
- These findings suggest that increased JUN expression and activity may contribute to gefitinib resistance in non-small cell lung cancer. PMID: 28566434
- The results indicated that butein has antiproliferative and proapoptotic properties through the suppression of NF-kappaB, AP-1 and Akt signaling in HTLV-1-infected T cells, both in vitro and in vivo, suggesting its therapeutic potential against HTLV-1-associated diseases including adult T-cell leukemia/lymphoma PMID: 28586006
- Results show that VEGFA induces c-jun expression in mediating human retinal microvascular endothelial cell migration, sprouting and tube formation, and that Pyk2-STAT3 signaling enhances cJun expression in the mediation of retinal neovascularization. PMID: 27210483
- Increased c-jun expression is associated with nasopharyngeal carcinoma. PMID: 28269757
- Thrombin binding to PAR-1 receptor activated Gi-protein/c-Src/Pyk2/EGFR/PI3K/Akt/p42/p44 MAPK cascade, which in turn elicited AP-1 activation and ultimately evoked MMP-9 expression and cell migration in SK-N-SH cells. PMID: 27181591
- Findings provide evidence that phospho-c-Jun activates an important regulatory mechanism to control DNMT1 expression and regulate global DNA methylation in glioblastoma. PMID: 28036297
- Results demonstrated for the first time the regulatory mechanism of miR-744 transcription by c-Jun, providing a potential mechanism underlying the upregulation of miR-744 in cancers PMID: 27533465
- Results provide evidence that NuRD represses c-Jun transcription directly which, in the absence of MBD3, activates endogenous pluripotent genes and regulates induced cancer stem cells-related genes. PMID: 27894081
- Taken together, these results indicated that PAR1 signaling-mediated cJun activation promotes early apoptosis of HUVEC cells induced by heat stress. PMID: 28447716
- Cheliensisin A (Chel A) treatment led to PH domain and Leucine rich repeat Protein Phosphatases (PHLPP2) protein degradation and subsequently increased in c-Jun phosphorylation, which could be attenuated by inhibition of autophagy mediated by Beclin 1. PMID: 27556506
- The positive feedback regulation of OCT4 and c-JUN, resulting in the continuous expression of oncogenes such as c-JUN, seems to play a critical role in the determination of the cell fate decision from induced pluripotent stem cells to cancer stem cells in liver cancer. PMID: 27341307
- miR-26b plays an anti-metastatic role and is downregulated in gastric cancer tissues via the KPNA2/c-jun pathway PMID: 27078844
- The IL1B/AP-1/miR-30a/ADAMTS-5 axis regulates cartilage matrix degradation in osteoarthritis. PMID: 27067395
- TGM2 is involved in amyloid-beta (1-42)-induced pro-inflammatory activation via AP1/JNK signaling pathways in cultured monocytes. PMID: 27864692
- Integrative genomic analysis indicated overexpression of the AP-1 transcriptional complex suggesting experimental therapeutic rationales, including blockade of the renin-angiotensin system. This led to the repurposing of the angiotensin II receptor antagonist, irbesartan, as an anticancer therapy, resulting in the patient experiencing a dramatic and durable response. PMID: 27022066
- Knockdown of CD44 reduced the protein level of xCT, a cystine transporter, and increased oxidative stress. However, an increase in GSH was also observed and was associated with enhanced chemoresistance in CD44-knockdown cells. Increased GSH was mediated by the Nrf2/AP-1-induced upregulation of GCLC, a subunit of the enzyme catalyzing GSH synthesis PMID: 28185919
- Study highlights the role of AP1 in promoting the host gene expression profile that defines Ebola virus pathogenesis. PMID: 28931675
- This is the first study to show how TGF-beta regulates the expression of Claudin-4 through c-Jun signaling and how this pathway contributes to the migratory and tumorigenic phenotype of lung tumor cells. PMID: 27424491
- Data show that BRD4 controls RUNX2 by binding to the enhancers (ENHs) and each RUNX2 ENH is potentially controlled by a distinct set of TFs and c-JUN as the principal pivot of this regulatory platform. PMID: 28981843
- AP-1 likely plays a more important role in the AR cistrome in fibroblasts. PMID: 27634452
- Elevated levels of bile acid increase the tumorigenic potential of pancreatic cancer cells by inducing FXR/FAK/c-Jun axis to upregulate MUC4 expression. PMID: 27185392
- Immunohistochemistry was employed to analyze cFos, cJun and CD147 expression in 41 UCB cases and 34 noncancerous human bladder tissues. PMID: 28358415
- Taken together, these findings indicate that LT reduces c-Jun protein levels via two distinct mechanisms, thereby inhibiting critical cell functions, including cellular proliferation. PMID: 28893904
- Expression of either dominant-negative or constitutively active mutants of Nrf2, ATF4, or c-Jun confirmed that distinct transcription units are regulated by these transcription factors. PMID: 27278863
- Mutually exclusive transcriptional regulation by AP-1 (cjun/cfos) and non-canonical NF-kappaB (RelB/p52) downstream of MEK-ERK and NIK-IKK-alpha-NF-kappaB2 (p100) phosphorylation, respectively, was responsible for persistent Ccl20 expression in the colonic cells. PMID: 27590109
- Glucocorticoid receptor (GR) is recruited to activator protein-1 (AP-1) target genes in a DNA-binding-dependent manner. PMID: 28591827
- These results suggested that hyperphosphatemia in the patients with CKD suppresses bone resorption by inhibiting osteoclastogenesis, and this impairs the regulation of bone metabolism. PMID: 28939042
- These results suggest that Bacteroides fragilis enterotoxin induced accumulation of autophagosomes in endothelial cells, but activation of a signaling pathway involving JNK, AP-1, and CHOP may interfere with complete autophagy. PMID: 28694294
- Overall, our results suggest that miR-4632 plays an important role in regulating HPASMC proliferation and apoptosis by suppression of cJUN, providing a novel therapeutic miRNA candidate for the treatment of pulmonary vascular remodeling diseases. It also implies that serum miR-4632 has the potential to serve as a circulating biomarker for PAH diagnosis. PMID: 28701355
- Findings suggest that AP-1 factors are regulators of RNA polymerase III (Pol III)-driven 5S rRNA and U6 snRNA expression with a potential role in cell proliferation. PMID: 28488757
- Our results indicate that assessing AP1 and PEA3 transcription factor status might be a good indicator of OAC status. However, we could not detect any associations with disease stage or patient treatment regime. This suggests that the PEA3-AP1 regulatory module more likely contributes more generally to the cancer phenotype. In keeping with this observation, depletion of ETV1 and/or ETV4 causes an OAC cell growth defect PMID: 28859074
- shRNA-mediated inhibition of JUN decreases AML cell survival and propagation in vivo. These data uncover a previously unrecognized role of JUN as a regulator of the unfolded protein response PMID: 27840425
- These findings demonstrate an essential role for the ERK pathway together with c-JUN and c-FOS in the differentiation activity of LukS-PV. PMID: 27102414
- The present study defines the minimal TIM-3 promoter region and demonstrates its interaction with c-Jun during TIM-3 transcription in CD4(+) T cells. PMID: 27243212
- Taken together, our data demonstrate that JNK regulates triple-negative breast cancer (TNBC) tumorigenesis by promoting CSC phenotype through Notch1 signaling via activation of c-Jun and indicate that JNK/c-Jun/Notch1 signaling is a potential therapeutic target for TNBC PMID: 27941886
- Regulation of osteosarcoma cell lung metastasis by the c-Fos/AP-1 target FGFR1 PMID: 26387545
- c-jun promoted FOXK1-mediated proliferation and metastasis via orthotopic implantation. PMID: 27882939
- Data provide evidence that AP-1 is a key determinant of endocrine resistance of breast cancer cells by mediating a global shift in the estrogen receptor transcriptional program. PMID: 26965145
- Comparison of how AP-1 (Jun/Jun dimer) and Epstein-Barr virus Zta recognize methyl groups within their cognate response elements PMID: 28158710
Q&A
Basic Research Questions
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What is JUN (Ab-91) Antibody and what does it specifically target?
JUN (Ab-91) Antibody is a polyclonal antibody produced in rabbits that specifically targets the c-Jun protein (also known as Activator Protein 1, or AP-1). It recognizes the peptide sequence around amino acids 89-93 (T-T-T-P-T) derived from human c-Jun . The antibody detects endogenous levels of total c-Jun protein, which is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3' . c-Jun is a critical component of the AP-1 transcription complex and plays important roles in cellular proliferation, differentiation, and apoptosis .
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What research applications is the JUN (Ab-91) Antibody validated for?
The JUN (Ab-91) Antibody has been validated for multiple molecular biology applications:
The antibody has been cited in scientific literature for applications such as investigating the down-regulation of TRPM6-mediated magnesium influx by cyclosporin A .
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What species reactivity has been confirmed for JUN (Ab-91) Antibody?
The JUN (Ab-91) Antibody has been confirmed to react with samples from the following species:
This cross-species reactivity makes it valuable for comparative studies across different mammalian model systems.
Intermediate Research Questions
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What are the recommended storage conditions and handling protocols for optimal stability?
For optimal stability and performance of the JUN (Ab-91) Antibody:
The antibody is typically supplied in a formulation of 1.0mg/mL in phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, 150mM NaCl, 0.02% sodium azide, and 50% glycerol . This formulation helps maintain antibody stability during storage.
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How can I optimize Western blot protocols when using JUN (Ab-91) Antibody?
For optimal Western blot results with JUN (Ab-91) Antibody:
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For sample preparation, standard protein extraction methods from cells or tissues are compatible
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Include positive controls (cell lines known to express c-Jun) and negative controls (samples where c-Jun is not expressed or knocked down)
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For detection, standard secondary antibodies against rabbit IgG conjugated with HRP or other detection systems are suitable
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When analyzing results, ensure specificity by confirming band size matches the expected molecular weight of c-Jun (~43 kDa, though some references note ~43-52 kDa range)
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What controls should I include when using JUN (Ab-91) Antibody in immunohistochemistry?
When performing immunohistochemistry with JUN (Ab-91) Antibody:
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Positive tissue controls: Use tissues known to express c-Jun (such as specific cancer cell lines or tissues with active AP-1 signaling)
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Negative controls:
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Omit primary antibody but include all other steps
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Use tissues known to lack c-Jun expression
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Use isotype control (rabbit IgG at the same concentration)
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Blocking controls: Pre-incubate the antibody with the immunizing peptide
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For formalin-fixed, paraffin-embedded sections, standard antigen retrieval methods are compatible
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Nuclear staining pattern should be observed given c-Jun's function as a transcription factor
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Research Applications and Significance
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What biological processes and signaling pathways can be studied using JUN (Ab-91) Antibody?
The JUN (Ab-91) Antibody can be used to investigate numerous important biological processes:
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AP-1 transcriptional regulation: c-Jun is a component of the AP-1 complex that regulates gene expression following growth factor responses
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Cell signaling cascades:
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MAPK/JNK pathway activation leads to c-Jun phosphorylation
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Growth factor signaling pathways that activate AP-1
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Cell fate decisions:
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Developmental processes:
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Pathological conditions:
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How can I incorporate JUN (Ab-91) Antibody in single-cell analysis techniques?
For incorporating JUN (Ab-91) Antibody in single-cell analysis:
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For single-cell immunofluorescence:
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For flow cytometry applications:
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Optimize permeabilization protocols for intracellular staining
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Use appropriate isotype controls and fluorescence-minus-one (FMO) controls
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Consider fixation-resistant fluorophores for secondary antibodies
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For high-content imaging:
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Develop quantitative image analysis workflows to measure nuclear vs. cytoplasmic c-Jun localization
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Correlate c-Jun levels with other cellular markers in multiplex imaging
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Data analysis considerations:
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Account for cellular heterogeneity in c-Jun expression
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Correlate c-Jun activation with cell cycle phases or differentiation states
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Consider population-level statistics alongside single-cell measurements
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