nkiras1 Antibody

Introduction to NKIRAS1 Antibody

The NKIRAS1 Antibody is a specialized immunological tool designed to detect the NF-κB Inhibitor Interacting Ras-like 1 (NKIRAS1) protein, a tumor suppressor involved in modulating NF-κB and Ras signaling pathways. This antibody is critical for studying NKIRAS1's role in cancer biology, inflammation, and cellular stress responses. NKIRAS1 functions by stabilizing IκB inhibitors, thereby suppressing NF-κB activation, and indirectly inhibiting Ras-mediated oncogenic signaling .

Key Research Findings

NKIRAS1's role as a tumor suppressor has been validated through pan-cancer analyses and experimental models:

Table 1: Clinical Relevance of NKIRAS1 Downregulation in Human Cancers

• Mechanistic Insights:

  • NKIRAS1 loss promotes epithelial-mesenchymal transition (EMT) and DNA damage repair deficiencies .

  • Reduced NKIRAS1 correlates with upregulated miR-154, which targets PCNA, a gene critical for HBV replication and carcinogenesis .

Immunohistochemistry (IHC)

• NKIRAS1 antibodies detect protein expression in formalin-fixed, paraffin-embedded (FFPE) tissues, with staining observed in hepatocellular carcinoma (HCC) and adjacent non-tumor tissues .

• Example Protocol:

  • Dilution: 1:100–1:2500 .

  • Validation: Confirmed via TCGA dataset correlations (e.g., PCNA overexpression in HBV-related HCC) .

Western Blot (WB)

• Antibodies like ABIN500110 identify both κB-Ras1 and κB-Ras2 isoforms, with bands at ~25 kDa .

Functional Studies

• miRNA Interactions: NKIRAS1 downregulation is mediated by miR-154, miR-155, and a network of 11 miRNAs that also target tumorigenic genes (e.g., ARID4B, TAX1BP1) .

• Pathway Analysis: NKIRAS1 loss activates pro-inflammatory (NF-κB) and pro-proliferative (Ras/MAPK) pathways, driving tumor progression .