KCNJ8 Antibody, Biotin conjugated
Description
Introduction to KCNJ8 Antibody, Biotin Conjugated
The KCNJ8 Antibody, Biotin conjugated is a specialized immunological reagent designed to detect the potassium inwardly rectifying channel subfamily J member 8 (KCNJ8), also known as Kir6.1. This antibody leverages biotin conjugation for enhanced detection sensitivity in applications such as Western blotting (WB), immunohistochemistry (IHC), and enzyme-linked immunosorbent assay (ELISA). KCNJ8/Kir6.1 is a critical subunit of ATP-sensitive potassium channels, influencing cellular responses to metabolic stress and ion homeostasis . Below is a detailed analysis of its characteristics, applications, and research findings.
Immunogen and Specificity
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Target Epitope: The antibody binds to distinct regions of the KCNJ8 protein, including amino acids 306–424 (cytoplasmic C-terminus) in rat Kir6.1 and 299–424 in human KCNJ8 .
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Reactivity:
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Conjugate: Biotin enables streptavidin-based detection systems, enhancing signal amplification in assays.
Applications
Successful Detection in Biological Samples
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ABIN2485710:
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PACO57095/QA54269:
Future Directions and Research Gaps
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NK Cell Studies: Investigate KCNJ8 expression in NK cell subsets using ABIN2485710 for IHC or flow cytometry .
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Cross-Species Validation: Expand PACO57095/QA54269’s reactivity to non-human models.
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Multiplex Assays: Leverage biotin conjugation for simultaneous detection of KCNJ8 with other ion channels.
Product Specs
Target Background
This antibody targets a G protein-regulated potassium channel. Inward rectifier potassium channels are distinguished by their preferential conductance of potassium ions into, rather than out of, the cell. Their voltage dependence is modulated by extracellular potassium concentration; increasing external potassium shifts the channel activation voltage range to more positive potentials. Inward rectification is primarily attributed to intracellular magnesium block of outward current. The channel is susceptible to blockade by extracellular barium.
The following studies provide insights into the function and clinical relevance of KCNJ8:
- A study on KCNJ8-S422L and its association with J-wave syndromes suggests the variant's pathogenicity may be dependent on population structure, with Ashkenazi Jews potentially exhibiting increased risk. PMID: 23632791
- A de novo missense mutation (Kir6.1[p.Cys176Ser]) in KCNJ8 demonstrated significantly enhanced channel activity compared to wild-type, due to reduced ATP sensitivity. PMID: 24700710
- Research indicates upregulation of KATP channels with age in human myometrium. PMID: 23369859
- Pharmacological or siRNA-mediated inhibition of KvLQT1 and KATP (Kir6.1) downregulated alpha-ENaC expression. PMID: 22406554
- The KCNJ8-S422L variant has been linked to increased susceptibility to both atrial fibrillation and early repolarization syndrome. PMID: 22562657
- Methylglyoxal affects Kir6.1 and SUR2B mRNA stability, disrupting vascular KATP channels and arterial function. PMID: 22972803
- Kir6.1 and the M3 muscarinic receptor colocalize to detrusor caveolae. PMID: 22410194
- KCNJ8 has been implicated in increasing susceptibility to Brugada syndrome and early repolarization syndrome. PMID: 22056721
- Mutations in Kir6.1's C-terminus, affecting conserved residues, decreased pinacidil-activated KATP current. PMID: 21836131
- Downregulation of Kir6.1 and Kir6.2 in myometrium may contribute to enhanced uterine contractility during labor. PMID: 21418633
- Caveolin-1 interaction alters the channel's sensitivity to magnesium ADP (MgADP). PMID: 20624795
- KCNJ8-S422L is associated with J-wave syndromes via a gain-of-function mechanism in the cardiac KATP Kir6.1 channel. PMID: 20558321
- Mammalian oocytes express KATP channels. PMID: 20847183
- KCNJ8 sequence variants are unlikely to influence postural changes in systolic blood pressure. PMID: 19952277
- Lipopolysaccharides upregulate Kir6.1/SUR2B channel expression and enhance vascular KATP channel activity via NF-kappaB signaling. PMID: 19959479
- Channel assembly influences the pharmacological properties of ATP-sensitive potassium channels. PMID: 11825905
- cGMP/PKG pathways are involved in activating ATP-regulated potassium channels. PMID: 12217870
- Downregulation of this channel may be involved in myometrial function during late pregnancy. PMID: 12356945
- Corporal smooth muscle KATP channels consist of Kir6.1-Kir6.2 heteromultimers with SUR2B. PMID: 12934053
- Kir6.1/KCNJ8 plays a role in coronary vasomotility, with ethnic variations observed. PMID: 12964027
- Nicotine's effects on Kir6.1 channels are mediated by superoxide production. PMID: 15821440
- The Kir6.1-SUR2A complex regulates paracellular permeability through tight junctions. PMID: 16820413
- Primary Kir6.1 structural abnormalities may not be involved in coronary spastic angina pathogenesis. PMID: 16964409
- PKC-epsilon inhibits vascular KATP channels (Kir6.1 and SUR2B) via caveolin-dependent internalization. PMID: 18663158
- Kir6.1/SUR2B is the major KATP channel complex in pig and likely human middle meningeal arteries. PMID: 18996111
- Analysis of KCNJ11 and KCNJ8 mutations reveals differences between channel subtypes formed with SUR1. PMID: 19139106
