KIN10 Antibody
Shipped with Ice Packs 
In Stock 
Product Specs
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
Made-to-order (14-16 weeks)
Synonyms
KIN10 antibody; AK21 antibody; AKIN10 antibody; SKIN10 antibody; SNR2 antibody; SNRK1.1 antibody; At3g01090 antibody; T4P13.22SNF1-related protein kinase catalytic subunit alpha KIN10 antibody; AKIN10 antibody; EC 2.7.11.1 antibody; AKIN alpha-2 antibody; AKINalpha2 antibody; SNF1-related kinase 1.1 antibody; SnRK1.1 antibody
Target Names
KIN10
Uniprot No.
Target Background
Function
KIN10 is the catalytic subunit of the probable trimeric SNF1-related protein kinase (SnRK) complex. This complex plays a pivotal role in cellular energy homeostasis. It responds to diverse stimuli including darkness, sugar fluctuations, and stress conditions. This response leads to the activation of energy-producing pathways and the inhibition of energy-consuming processes. KIN10 is believed to be involved in signal transduction cascades that regulate gene expression and carbohydrate metabolism in higher plants. The SnRK complex is also thought to participate in regulating fatty acid synthesis by phosphorylating acetyl-CoA carboxylase, and in nitrogen assimilation by phosphorylating nitrate reductase.
In vitro studies have shown that KIN10 exhibits kinase activity towards sucrose phosphate synthase. This activity is inhibited by PRL1. KIN10 may be a subunit of a SCF ubiquitin ligase complex, potentially involved in proteasomal ubiquitination. It has been observed to phosphorylate GRIK1/SNAK2 and GRIK2/SNAK1 in vitro.
KIN10 cooperates with FUS3 to regulate developmental phase transitions and lateral organ development, acting as positive regulators of abscisic acid (ABA) signaling during germination. It phosphorylates FUS3 within the embryo. KIN10 negatively modulates MYC2 accumulation through protein phosphorylation. It also phosphorylates the AL2 protein of geminiviruses (CaLCuV, TGMV, ToMoV), leading to a delay in viral DNA accumulation and symptom appearance during infection.
KIN10 regulates bZIP63 activity to alter metabolism in response to starvation through protein phosphorylation. Under sugar deprivation conditions, KIN10 antagonizes the IDD8 function in flowering time control by phosphorylating it. KIN10 plays a crucial role in the control of cell proliferation through inhibiting KRP6 activity by phosphorylation. During submergence, KIN10 phosphorylates PTP1, leading to the release of the MPK6 signaling pathway inhibition. KIN10 triggers its own SUMO-mediated proteasomal degradation, establishing a negative feedback loop that attenuates SnRK1 signaling and prevents excessive activation of stress responses.
KIN10 phosphorylates RAPTOR1B and HMGR1S in vitro. Its kinase activity is sensitive to redox changes. KIN10 acts upstream of TOR in the regulation of autophagy. It is essential for the activation of autophagy by various abiotic stresses. KIN10 is involved in the positive regulation of autophagy, potentially by affecting the phosphorylation of ATG1 proteins. It negatively modulates WRI1 accumulation through protein phosphorylation. KIN10 modulates leaf senescence progression by negatively regulating EIN3 accumulation through phosphorylation. Under extended darkness, the C/S1-bZIP-SnRK1 complex interacts with histone acetylation machinery to remodel chromatin and facilitate transcription. The BZIP2-BZIP63-KIN10 complex binds to the ETFQO promoter to upregulate its transcription. Finally, KIN10 phosphorylates and downregulates IPK2b in vitro. It is involved in the regulation of sucrose-induced hypocotyl elongation under light/dark cycles.
In vitro studies have shown that KIN10 exhibits kinase activity towards sucrose phosphate synthase. This activity is inhibited by PRL1. KIN10 may be a subunit of a SCF ubiquitin ligase complex, potentially involved in proteasomal ubiquitination. It has been observed to phosphorylate GRIK1/SNAK2 and GRIK2/SNAK1 in vitro.
KIN10 cooperates with FUS3 to regulate developmental phase transitions and lateral organ development, acting as positive regulators of abscisic acid (ABA) signaling during germination. It phosphorylates FUS3 within the embryo. KIN10 negatively modulates MYC2 accumulation through protein phosphorylation. It also phosphorylates the AL2 protein of geminiviruses (CaLCuV, TGMV, ToMoV), leading to a delay in viral DNA accumulation and symptom appearance during infection.
KIN10 regulates bZIP63 activity to alter metabolism in response to starvation through protein phosphorylation. Under sugar deprivation conditions, KIN10 antagonizes the IDD8 function in flowering time control by phosphorylating it. KIN10 plays a crucial role in the control of cell proliferation through inhibiting KRP6 activity by phosphorylation. During submergence, KIN10 phosphorylates PTP1, leading to the release of the MPK6 signaling pathway inhibition. KIN10 triggers its own SUMO-mediated proteasomal degradation, establishing a negative feedback loop that attenuates SnRK1 signaling and prevents excessive activation of stress responses.
KIN10 phosphorylates RAPTOR1B and HMGR1S in vitro. Its kinase activity is sensitive to redox changes. KIN10 acts upstream of TOR in the regulation of autophagy. It is essential for the activation of autophagy by various abiotic stresses. KIN10 is involved in the positive regulation of autophagy, potentially by affecting the phosphorylation of ATG1 proteins. It negatively modulates WRI1 accumulation through protein phosphorylation. KIN10 modulates leaf senescence progression by negatively regulating EIN3 accumulation through phosphorylation. Under extended darkness, the C/S1-bZIP-SnRK1 complex interacts with histone acetylation machinery to remodel chromatin and facilitate transcription. The BZIP2-BZIP63-KIN10 complex binds to the ETFQO promoter to upregulate its transcription. Finally, KIN10 phosphorylates and downregulates IPK2b in vitro. It is involved in the regulation of sucrose-induced hypocotyl elongation under light/dark cycles.
Gene References Into Functions
- KIN10 expression modulates root phenotype of Arabidopsis thaliana root system in condition of energy stress. PMID: 30480412
- KIN10 is required for sucrose-induced hypocotyl elongation in light/dark cycles. PMID: 29114081
- AKIN10 conditionally works in a circadian clock input pathway to the circadian oscillator. AKIN10 modulates the Arabidopsis circadian clock in a light-dependent manner. PMID: 28054361
- The activity and T-loop phosphorylation of AKIN10, the kinase subunit of the SnRK1 complex, is regulated by the redox status. PMID: 28940407
- SnRK1 acts upstream of TOR in the activation of autophagy in Arabidopsis PMID: 28783755
- Data suggest that AKIN10 phosphorylates and inactivates HMGR; AKIN10 phosphorylates not only conserved Ser577 in the active site but also other site/sites in the noncatalytic domain of HMGR; inactivation of HMGR by AKIN10 requires interaction of AKIN10 with GRIK1. (AKIN10 = SnRK1 protein kinase; HMGR = hydroxymethylglutaryl-CoA-reductase; GRIK1 = geminivirus Rep-interacting kinase 1) PMID: 28263378
- SnRK1 shares clear structural and functional similarities with its orthologs, yeast SNF1 and mammalian AMPK, but has evolved unique features that presumably provide a better adaptation to an autotrophic lifestyle. (Review) PMID: 27812990
- Taken together, our results disclose the central low-energy activated SnRK1-C/S1-bZIP signaling module as a gateway to integrate information on the plant's energy status into root meristem control, thereby balancing plant growth and cellular energy resources. PMID: 28158182
- Observations indicate that AKIN10 antagonizes the IDD8 function in flowering time control, a notion that is consistent with the delayed flowering phenotypes of AKIN10-overexpressing plants and idd8-3 mutant PMID: 25929516
- AKIN10 negatively modulates AtMYC2 protein accumulation via proteasome activity upon AKIN10 kinase activity-dependent protein modification. Transgenic plants expressing AKIN10 indicate that AKIN10 activity undermined AtMYC2-dependent salt tolerance. PMID: 24890857
- Arabidopsis SnRK1.1 phosphorylation of AL2 delays Cabbage leaf curl virus infection. PMID: 24990996
- SnRK1 activities critically influence stress-inducible gene expression and the induction of stress tolerance. [SnRK1.1] PMID: 22232383
- T6P/SnRK1 signaling pathway responds to Suc induced by sink restriction that enables growth recovery following relief of limitations such as low temperature. [SnRK1] PMID: 23735508
- FUS3 and AKIN10 functionally overlap in abscissic acid signaling, but play different roles in sugar responses during germination. PMID: 22902692
- FUS3 is phosphorylated by AKIN10. AKIN10 overexpression delays FUS3 degradation. AKIN10 and FUS3 function interactively to promote seed maturation, dormancy and inhibit developmental phase transitions. PMID: 22026387
- Data show that PP2C74 interacts with AKIN10, the catalytic alpha subunit of the SnRK1 protein kinase complex, the beta subunits of which are known targets of myristoylation. PMID: 22449965
- These studies uncover surprisingly pivotal roles of KIN10/11 in linking stress, sugar and developmental signals to globally regulate plant metabolism, energy balance, growth and survival PMID: 17671505
- Upon sensing the energy deficit associated with stress, nutrient deprivation and darkness, SnRK1 triggers extensive transcriptional changes that contribute to restoring homeostasis, promoting cell survival, adaptation, growth and development. PMID: 18701338
- SnRK1 is negatively regulated by 5PTase13. PMID: 18931139
- Trehalose-6-phosphate inhibits SnRK1 to activate biosynthetic processes in growing tissues. PMID: 19193861
- This finding reveals novel roles for the different catalytic subunits AKIN10 and AKIN11 during phosphate starvation. PMID: 19211700
- SnRK1.1 is activated by phosphorylation in response to sugars, and over-expression confers a glucose- and ABA-hypersensitive phenotype. PMID: 19302419
- GRIKs are SnRK1 activating kinases. PMID: 19339507
Database Links
Protein Families
Protein kinase superfamily, CAMK Ser/Thr protein kinase family, SNF1 subfamily
Subcellular Location
[Isoform 1]: Plastid, chloroplast. Cytoplasm. Nucleus. Golgi apparatus.; [Isoform 2]: Cytoplasm. Nucleus. Endoplasmic reticulum.
Tissue Specificity
Isoform 2 is widely expressed, especially in newly developing tissues. Isoform 2 is expressed throughout the seedling, with highest expression in leaf primordia and vascular tissue, and the seedling root tip. Isoform 2 is later expressed in developing lat
Quick Inquiry
Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *
