KIN14N Antibody

Introduction to KIN14N Antibody

The KIN14N Antibody (Product Code: CSB-PA793971XA01OFG) is a polyclonal antibody developed for the detection of the KIN14N protein in Oryza sativa subsp. japonica (rice). KIN14N is a member of the kinesin motor protein family, which plays critical roles in intracellular transport, mitosis, and cytoskeletal organization. Antibodies targeting KIN14N enable researchers to study its expression, localization, and functional interactions in plant systems .

Validation Data

• Western Blot: Detects a band at the predicted molecular weight (~75–85 kDa) in rice lysates .

• Immunohistochemistry: Localizes KIN14N to microtubule-rich regions in rice root tip sections .

Role of KIN14N in Plant Cells

Kinesin-14 family proteins, including KIN14N, are minus-end-directed microtubule motors involved in:

1. Cytoskeletal Organization: Facilitating microtubule bundling and spindle formation during mitosis .

2. Intracellular Transport: Mediating retrograde transport of organelles and vesicles .

3. Cell Division: Regulating chromosome segregation and cell plate formation .

Functional Insights from Orthologs

• In Physcomitrium patens, the kinesin-14 motor kin14-VIb achieves processive motility when clustered, enabling long-range retrograde transport (up to 600 nm/s) .

• Animal kinesin-14 homologs (e.g., human KIF14) regulate cell migration and focal adhesion dynamics via integrin activation .

Validation and Quality Control

Example Workflow for KIN14N Studies

1. Sample Preparation: Extract proteins from rice seedlings under stress conditions.

2. Western Blot: Use KIN14N Antibody (1:500 dilution) to assess expression changes.

3. Immunohistochemistry: Localize KIN14N in root sections using fluorescence microscopy .

Challenges and Future Directions

1. Antibody Validation: Current data lack in planta functional knockout validation. CRISPR/Cas9-generated KIN14N mutants would clarify its role .

2. Cross-Species Reactivity: Expand testing to Oryza sativa subsp. indica and other monocots.

3. Therapeutic Potential: Explore KIN14N’s role in stress responses (e.g., drought, pathogen invasion) .

References

1. IgG Subclasses and Effector Functions (PMC4202688) .

2. Processive Motility of Kinesin-14 (PMC4548964) .

3. Antibody Characterization Standards (eLife 100211) .

4. Cusabio KIN14N Antibody Documentation .