Kpnb1 Antibody
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Description
Definition and Function of Kpnb1 Antibody
The Kpnb1 antibody is a research tool designed to detect and study the Karyopherin beta 1 (Kpnb1) protein, a critical nuclear transport receptor. Kpnb1 facilitates the import of proteins containing nuclear localization signals (NLS) into the nucleus, often in conjunction with importin alpha adaptors . The antibody is widely used in molecular biology for techniques such as Western blotting, immunoprecipitation, and immunofluorescence to analyze Kpnb1 expression and localization in diverse cell types .
Research Applications and Findings
Table 2: Research Highlights Using Kpnb1 Antibody
Mechanistic Insights:
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Cancer progression: Overexpression of Kpnb1 correlates with poor prognosis in EOC and AML .
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Therapeutic targeting: Importazole, a small-molecule inhibitor, inhibits Kpnb1 function, enhancing chemotherapy efficacy .
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Proteostasis regulation: Kpnb1 maintains protein homeostasis; its inhibition disrupts nuclear import of cargoes like APC/C and HMGB2 .
Validation and Specificity
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Epitope specificity: The BAR-based antibody (residues 301-320) distinguishes cytoplasmic Kpnb1 from nuclear pools, enabling precise subcellular analysis .
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Cross-reactivity: Antibodies from Abcam and Cell Signaling cross-react with human, mouse, and rat Kpnb1 (97%–99% sequence identity) .
Future Directions
Product Specs
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
Made-to-order (12-14 weeks)
Synonyms
Kpnb1 antibody; Impnb antibody; Importin subunit beta-1 antibody; Karyopherin subunit beta-1 antibody; Nuclear factor p97 antibody; Pore targeting complex 97 kDa subunit antibody; PTAC97 antibody; SCG antibody
Target Names
Kpnb1
Uniprot No.
Target Background
Function
Importin beta 1 (KPNB1) is a crucial protein involved in nuclear protein import. It functions either in association with an adapter protein, such as an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as an autonomous nuclear transport receptor. When acting autonomously, it serves as an NLS receptor itself. The docking of the importin/substrate complex to the nuclear pore complex (NPC) is facilitated by KPNB1 through its binding to nucleoporin FxFG repeats. Subsequently, the complex is translocated through the pore via an energy-requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta, leading to the separation of the three components. Importin-alpha and -beta are then re-exported from the nucleus to the cytoplasm, where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is believed to be conferred by the asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. KPNB1 autonomously mediates the nuclear import of ribosomal proteins RPL23A, RPS7, and RPL5. It binds to a beta-like import receptor binding (BIB) domain of RPL23A. In conjunction with IPO7, KPNB1 mediates the nuclear import of H1 histone. In vitro, KPNB1 mediates the nuclear import of H2A, H2B, H3, and H4 histones. Additionally, it imports SNAI1 and PRKCI into the nucleus.
Gene References Into Functions
- KPNB1 acts as a positive regulator in the NF-kappaB pathway to enhance palmitate-induced inflammation response. PMID: 26452501
- KPNB1 may play a key role in the inflammation process of rheumatoid arthritis via STAT3 signal transduction pathway. PMID: 26879143
- The study revealed a regulatory role of the p97-Npl4-Ufd1 complex in regulating a partial degradation of the NF-kappaB subunit p100. PMID: 26112410
- Ei24 can bind specifically to IMPbeta1 and IMPalpha2 to impede their normal role in nuclear import. PMID: 24821838
- Identification of a karyopherin beta1/beta2 proline-tyrosine nuclear localization signal in huntingtin protein. PMID: 23012356
- Translation of Importin beta1 mRNA enables separation of cytoplasmic and nuclear transport functions of importins and is required for efficient retrograde signaling in injured axons. PMID: 22841314
- A critical function of RanBP2 is to capture recycling RanGTP-importin-beta complexes at cytoplasmic fibrils to allow for adequate classical nuclear localization signal-mediated cargo import. PMID: 21859863
- Ap4A hydrolase was found to translocate into the nuclei of mast cells following immunological activation; found its immunologically dependent association with importin beta PMID: 21402779
- Data show that MRTF-A contains an unusually long bipartite nuclear localisation signal embedded within the RPEL domain, that uses the importin (Imp)alpha/beta-dependent import pathway, and that import is inhibited by G-actin. PMID: 20818336
- Results show the crystal structure of importin-beta complexed with the active form of SREBP-2 PMID: 14645851
- Results suggest that the importin alpha/beta system is involved in nuclear entry of mammalian clock components Cry2 and Per2, which is indispensable to transcriptional oscillation of clock genes PMID: 15689618
- Importin-beta features many more binding spots for nucleoporins than suspected so far. PMID: 16338415
- These results demonstrated that the cause of post-implantation lethality of Ayu8108(geo/geo) homozygous embryos was impaired expression of the Kpnb1 gene. PMID: 16414015
- PTH1R forms a complex with the transport regulatory proteins, importin alpha(1) and importin beta, and nuclear PTH1R is associated with the nucleoplasm PMID: 16574786
- Importin beta1 protein is detected in selected germ and somatic cells in fetal gonads, with a striking perinuclear staining evident from embryonic day (E) 14.5 within testicular gonocytes PMID: 17654710
- Reduction of the affinity by RanGTP really occurs at the nucleoplasmic side of the entire nuclear pore complex PMID: 18845677
- Data show that importin beta and the integral nuclear pore glycoprotein Nup62 interact with hsp90, hsp70, p23, and the TPR domain proteins FKBP52 and PP5 during nuclear transport. PMID: 19581287
Database Links
Protein Families
Importin beta family, Importin beta-1 subfamily
Subcellular Location
Cytoplasm. Nucleus envelope.
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