KPTN Antibody
Description
Introduction to KPTN Antibody
The KPTN antibody (Catalog No. 16094-1-AP) is a rabbit-derived IgG antibody targeting the human, mouse, and rat kaptin protein. KPTN, also known as Actin-associated protein 2E4, is involved in actin reorganization during platelet activation and neuromorphogenesis . This antibody is widely used in techniques such as Western blot (WB), immunohistochemistry (IHC), immunofluorescence/immunocytochemistry (IF/ICC), and ELISA .
Validated Applications and Tissues
| Technique | Detected Samples |
|---|---|
| WB | HeLa cells, NIH/3T3 cells, human cerebellum, mouse skeletal muscle |
| IHC | Human kidney, heart, lung, ovary, placenta, skin, spleen |
| IF/ICC | HeLa cells |
The antibody’s specificity is demonstrated in Figure 1 of PMC10822905 , where it confirmed interactions between KPTN and OTUD3 in HEK-293T cells.
Key Research Findings and Mechanistic Insights
Recent studies highlight KPTN’s role in the mTORC1 signaling pathway and its regulation by deubiquitination:
Role in mTORC1 Signaling
These findings position KPTN as a critical regulator of nutrient-sensing pathways and a potential therapeutic target in cancers .
Future Directions
The KPTN antibody’s utility in uncovering mTORC1-related mechanisms underscores its importance in oncology and cell biology. Ongoing research focuses on:
Product Specs
Target Background
The KPTN Antibody plays a crucial role in the amino acid-sensing branch of the TORC1 signaling pathway. As part of the KICSTOR complex, it recruits, in an amino acid-independent manner, the GATOR1 complex to the lysosomal membranes. This facilitates the interaction between GATOR1, GATOR2, and the RAG GTPases. Functioning upstream of the RAG GTPases, KPTN is essential for negatively regulating mTORC1 signaling in the absence of amino acids. In the absence of the KICSTOR complex, mTORC1 is constitutively localized to the lysosome and activated. The KICSTOR complex is also likely involved in the regulation of mTORC1 by glucose.
- Research has identified a protein complex known as KICSTOR, composed of four proteins: KPTN, ITFG2, C12orf66, and SZT2. This complex is required for amino acid or glucose deprivation to inhibit mTORC1 in cultured human cells. PMID: 28199306
- Studies have shown that mutations in the KPTN gene, encoding kaptin, can cause a syndrome characterized by macrocephaly, neurodevelopmental delay, and seizures. PMID: 24239382
