KRT12 Antibody
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Description
Definition and Target
The KRT12 antibody targets keratin 12, a 54 kDa protein expressed primarily in corneal epithelial cells. It forms heterodimers with keratin 3 (KRT3) to create structural filaments essential for maintaining corneal strength and transparency . This antibody is widely used in ophthalmology and molecular biology to diagnose corneal dystrophies and investigate epithelial regeneration .
Host Species and Isotype
Reactivity
Molecular Weight
Common Techniques
Meesmann Corneal Dystrophy
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Genetic Link: Mutations in KRT12 (e.g., Leu132Pro) disrupt intermediate filament assembly, leading to corneal cyst formation .
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Imaging: KRT12 antibodies reveal abnormal keratin aggregates in MECD patient corneas via IHC .
Corneal Regeneration
Product Specs
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
Typically, we can ship products within 1-3 business days after receiving your order. Delivery times may vary depending on the purchasing method or location. Please contact your local distributor for specific delivery timeframes.
Synonyms
CK-12 antibody; Cytokeratin-12 antibody; K12 antibody; K1C12_HUMAN antibody; Keratin 12 antibody; Keratin antibody; Keratin; type I cytoskeletal 12 antibody; Keratin-12 antibody; KRT12 antibody; type I cytoskeletal 12 antibody
Target Names
Uniprot No.
Target Background
Function
KRT12 plays a crucial role in maintaining corneal epithelium organization, integrity, and keratin expression.
Gene References Into Functions
- Keratoconus (KC) can co-exist with granular corneal dystrophy (GCD). A missense mutation (c.370G > A) in the TGFBI gene and an insert mutation (c.1456-1457ins GAT) in the KRT12 gene were identified in a 23-year-old male patient with concurrent KC and GCD. PMID: 28567551
- In combination with an effective delivery vehicle, this siRNA approach presents a viable treatment option for preventing the Meesmann corneal dystrophy (MECD) pathology observed in K12-Leu132Pro heterozygous individuals. PMID: 24801514
- A novel missense mutation of the KRT12 gene was identified in Meesmann corneal dystrophy. In vivo confocal microscopy examinations revealed previously unreported depth-dependent ultrastructural changes in the living cornea. PMID: 24099278
- The Leu132Pro missense mutation resides within the helix-initiation motif of the keratin and is predicted to significantly alter the structure of the K12 protein. PMID: 23222558
- The lead siRNA, exhibiting an IC(50) of thirty picomolar, showed no keratin off-target effects or activation of TLR3 within the tested concentration ranges. PMID: 23233254
- A novel missense mutation within the highly conserved helix-initiation motif of KRT12 was identified as the cause of Meesmann's corneal dystrophy in a German family. PMID: 20577595
- A heterozygous Ala137Pro mutation in the keratin 12 gene was discovered in Japanese individuals with Meesmann's corneal dystrophy. PMID: 12543196
- A novel missense mutation (Y429C) in KRT12 led to MCD in 2 unrelated Taiwanese families. PMID: 16227835
- Mutations in the KRT12 gene are associated with Meesmann corneal dystrophy. PMID: 16352477
- A mutation associated with the symptomatic phenotype of Meesmann's corneal dystrophy results in the substitution of proline for arginine in the helix termination motif, potentially disrupting the normal helix structure and leading to a significant structural change of the keratin 12 protein. PMID: 17653038
- This is the second family recently diagnosed with Meesmann dystrophy in Denmark. This family represents a unique genotype, distinct from previously reported ones. All patients with microcysts were asymptomatic. PMID: 18245975
- The novel L433R mutation of the KRT12 gene, identified in two members of a Japanese family, caused Meesmann corneal dystrophy (MECD). PMID: 18661274
Database Links
Involvement In Disease
Corneal dystrophy, Meesmann (MECD)
Protein Families
Intermediate filament family
Tissue Specificity
Expressed in the corneal epithelium (at protein level).
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