KRT23 Antibody
Description
Overview of KRT23 Antibody
KRT23 (Keratin 23) is a type I intermediate filament protein encoded by the KRT23 gene on chromosome 17q21.2, playing roles in epithelial cell structure, differentiation, and cancer progression . Antibodies targeting KRT23 are critical tools for studying its expression and function in normal tissues and malignancies. These antibodies are validated for applications like Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF) .
Technical Features
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Immunogen: Recombinant human KRT23 fragments (e.g., amino acids 271–422) or fusion proteins .
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Storage: Stable at -20°C for 12 months; avoid freeze-thaw cycles .
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Validation: Specificity confirmed via ELISA, WB, and IHC across cell lines (e.g., HeLa, HepG2) and tissues (e.g., colon cancer, placenta) .
Role in Cancer Progression
Mechanistic Insights
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Proliferation: KRT23 depletion reduces replication/repair proteins (e.g., BRCA1, RAD51) and arrests cell cycle progression .
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Metastasis: Regulates epithelial-mesenchymal transition (EMT) via TGF-β/Smad2/3 phosphorylation .
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Immune Evasion: High KRT23 correlates with reduced CD8+ T cell recruitment, suggesting immunosuppressive roles .
Clinical and Therapeutic Implications
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Prognostic Marker: Elevated KRT23 correlates with shorter survival in colorectal and ovarian cancers .
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Therapeutic Target: siRNA-mediated KRT23 inhibition reduces tumor growth in vitro and in vivo .
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Drug Synergy: Combining KRT23 knockdown with melatonin or irradiation enhances antitumor effects .
Validation and Limitations
Product Specs
Product Science Overview
Cytokeratins are a group of intermediate filament proteins found in the intracytoplasmic cytoskeleton of epithelial tissues . They play a crucial role in providing structural support to cells, helping them resist mechanical stress . Cytokeratins are categorized into two types: acidic type I and basic or neutral type II . These proteins are expressed in a tissue-specific manner, making them valuable markers for identifying the cell of origin in various human tumors .
Cytokeratin 23 (CK23) is one of the lesser-known members of the cytokeratin family. Like other cytokeratins, CK23 is involved in maintaining the structural integrity of epithelial cells. The specific expression pattern of CK23 in different tissues and its role in various physiological and pathological processes are still under investigation. However, it is known that cytokeratins, including CK23, are commonly used as markers in the diagnosis and classification of epithelial tumors .
Mouse anti-human antibodies are secondary antibodies generated by immunizing mice with human antigens. These antibodies are then harvested and purified for use in various immunological assays . Mouse anti-human antibodies are widely used in research and diagnostic applications due to their high specificity and affinity for human antigens . They are commonly used in techniques such as immunohistochemistry (IHC), enzyme-linked immunosorbent assay (ELISA), and Western blotting .
The combination of mouse anti-human antibodies with cytokeratin 23 is particularly useful in the field of cancer research and diagnostics. By targeting CK23, researchers can identify and study the expression patterns of this protein in various epithelial tissues and tumors. This can provide valuable insights into the role of CK23 in cancer development and progression, as well as its potential as a diagnostic marker .
