KTI12 Antibody, Biotin conjugated

Introduction

The KTI12 Antibody, Biotin conjugated is a specialized immunological reagent designed to detect and study the KTI12 protein, a critical component of the Elongator complex involved in tRNA modification. This antibody is conjugated with biotin, enabling signal amplification via streptavidin or avidin-based detection systems in applications like ELISA, Western blotting (WB), and immunohistochemistry (IHC).

Applications

KTI12 Function

KTI12 is a PSTK-like tRNA-dependent ATPase that interacts with the Elongator complex to regulate tRNA anticodon modifications (e.g., cm5U34). These modifications are critical for ribosomal decoding fidelity and proteome stability . Structural studies reveal KTI12 binds tRNA in a manner analogous to O-phosphoseryl-tRNA kinase (PSTK), with ATP hydrolysis essential for Elongator’s enzymatic activity .

Role of the Biotin-Conjugated Antibody

• Mechanism: The biotin conjugate enables binding to streptavidin or avidin probes, amplifying detection signals in assays .

• Applications:

  • ELISA: Quantification of KTI12 protein levels in lysates .

  • Western Blot: Potential detection of KTI12 in complex samples (e.g., cell lysates) .

  • IHC: Localization of KTI12 in tissues (e.g., intrahepatic cholangiocarcinoma) .

Case Studies and Experimental Insights

1. ELISA Optimization:

   • Biotinylated KTI12 antibodies are paired with streptavidin-HRP for sensitive detection. Users must titrate dilutions to minimize background noise .

2. tRNA Modification Studies:

   • KTI12’s ATPase activity is essential for Elongator-mediated cm5U34 modifications. Mutations in KTI12 disrupt this process, highlighting its role in translational fidelity .

3. Antigen Retrieval:

   • For IHC, antigen retrieval with TE buffer (pH 9.0) or citrate buffer (pH 6.0) is recommended for unconjugated KTI12 antibodies .