LAMA4 Antibody, HRP conjugated
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Description
Definition and Target Specificity
The LAMA4 Antibody, HRP conjugated, is a horseradish peroxidase (HRP)-linked antibody targeting the laminin alpha 4 subunit, a key component of laminin-411 (α4β1γ1). This glycoprotein plays roles in cell adhesion, vascular development, and extracellular matrix remodeling .
Applications
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Western Blot (WB): Detects LAMA4 at 200–233 kDa in human placenta lysates .
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Immunohistochemistry (IHC): Localizes LAMA4 in human placenta tissue (Golgi apparatus) and T98G glioblastoma cells (cytoplasm/cell surfaces) .
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ELISA: Paired with capture antibodies for quantitative assays .
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Cell Adhesion Assays: Neutralizes laminin-411-mediated adhesion in HT1080 cells (ND₅₀: 1.5–7.5 µg/mL) .
4.1. Extracellular Matrix Remodeling
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Flow-induced endothelial remodeling decreases LAMA4 abundance at lysine residues in the LG3–LG4 linker region, as shown by proteomic and immunofluorescence analysis .
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Figure 1: Immunoblot of LAMA4 in endothelial cells under flow conditions .
4.2. Validation Data
Product Comparison
Validation and Quality Control
Product Specs
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
Typically, we can ship your order within 1-3 business days of receiving it. Delivery times may vary depending on the shipping method and location. Please contact your local distributor for specific delivery times.
Synonyms
CMD1JJ antibody; DKFZp686D23145 antibody; LAMA3 antibody; LAMA3, formerly antibody; LAMA4 1 antibody; LAMA4 antibody; LAMA4*-1 antibody; LAMA4_HUMAN antibody; Laminin alpha 4 antibody; Laminin alpha 4 chain antibody; Laminin subunit alpha 4 precursor antibody; Laminin subunit alpha-4 antibody; Laminin-14 subunit alpha antibody; Laminin-8 subunit alpha antibody; Laminin-9 subunit alpha antibody
Target Names
Uniprot No.
Target Background
Function
Laminin, known to bind to cells through a high-affinity receptor, is believed to play a crucial role in mediating cell attachment, migration, and tissue organization during embryonic development. This function is achieved by interacting with other extracellular matrix components.
Gene References Into Functions
- Elevated LAMA4 expression has been associated with gastric cancer. PMID: 30015861
- Disruptions in LAMA4, LAMA5, and LAMB2, or the interaction between laminin and its receptor, are implicated in neuromuscular disorders including Pierson syndrome and Lambert-Eaton myasthenic syndrome (LEMS). (Review) PMID: 27614294
- MCAM and LAMA4 are highlighted as prime candidates for prognostic evaluation and therapeutic targeting in renal cell carcinoma. PMID: 26921326
- LAMA4-integrin signaling influences chondrocyte morphology and gene expression, contributing to the formation of clusters in osteoarthritic chondrocytes. PMID: 26295200
- Reduced LAMA4 expression is observed in preeclamptic placentas. LAMA4 promotes trophoblast cell invasion, migration, and angiogenesis. Hypoxia-reoxygenation decreases LAMA4 expression and disrupts the relationship between LAMA4 expression and the activation of p38 and ERK signaling pathways. PMID: 26059342
- Oxidative stress plays a crucial role in regulating LAMA4 expression through MAPK signaling pathways, suggesting a potential pathological mechanism for pre-eclampsia. PMID: 25676580
- Laminins 411 and 421 exhibit differential effects on tumor cell migration, mediated by alpha6beta1 integrin and MCAM (CD146). PMID: 24951930
- Data obtained from experiments utilizing peptide fragments suggest that laminin alpha4 (and laminin alpha5) may be part of the host defense response and contribute to protecting tissues from invading pathogens. PMID: 20433883
- A comparison of laminins with alpha1, alpha4, and alpha5 chains reveals isoform-specific promotion of adhesion and migration of human bone marrow progenitor cells. PMID: 12393739
- Research has identified a splice variant of the human laminin alpha4 subunit transcript containing 21 extra nucleotides. PMID: 12445830
- The G domain of the laminin alpha4 chain acts as a specific, high-affinity ligand for alphavbeta3 & alpha3beta1 integrin heterodimers. These integrins, in collaboration with alpha6beta1, cooperatively mediate endothelial cell-alpha4 laminin interaction and contribute to blood vessel development. PMID: 12454288
- The alpha4 laminin subunit plays a regulatory role in vascular endothelium cell survival. PMID: 14980521
- The LG4 module of the laminin alpha4 chain may play a significant role in cell adhesion and/or vessel wall formation in the skin through interactions with syndecan-2 and/or -4. PMID: 15086543
- Downregulation of laminin alpha4 chain expression inhibits glioma invasion. PMID: 15915502
- Changes in laminin isoforms have been associated with brain tumor invasion and angiogenesis. [review] PMID: 16146715
- Findings suggest that proteins containing the G(1121-1139) peptide may perform their biological functions by interacting with alphavbeta3 integrin. PMID: 16824487
- Laminin alpha4 chain (Lms) exhibits a de-adhesive function, potentially contributing to the detachment, migration, and invasion of renal carcinoma cells in vivo. PMID: 17533363
- Two novel mutations (2828C>T [Pro943Leu] & 3217C>T [Arg1073X]) within the integrin-interacting domain of the LAMA4 gene reduce its integrin binding capacity, leading to endothelial cell loss and heart failure. PMID: 17646580
- LAMA4 is specifically upregulated at both the mRNA and protein levels in hepatocellular carcinoma, indicating a potential role in hepatocarcinogenesis and tumor progression. PMID: 18084776
- Concomitant changes occur in laminin- and collagen-binding receptors. Laminin-411 reduces adhesion to laminin-511 and fibronectin, suggesting that tumor cells might utilize laminin-411 during their invasive behavior. PMID: 18496706
- LM alpha4 and beta2 play roles in the in vitro migration and in vivo tumorigenicity of prostate cancer cells. PMID: 19048114
Database Links
Involvement In Disease
Cardiomyopathy, dilated 1JJ (CMD1JJ)
Subcellular Location
Secreted, extracellular space, extracellular matrix, basement membrane. Note=Major component.
Tissue Specificity
In adult, strong expression in heart, lung, ovary small and large intestines, placenta, liver; weak or no expression in skeletal muscle, kidney, pancreas, testis, prostate, brain. High expression in fetal lung and kidney. Expression in fetal and newborn t
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