LAMB1 Antibody, HRP conjugated
Description
Antibody Overview
LAMB1 antibodies target the beta-1 chain of laminin, which mediates cellular adhesion, migration, and tissue organization . HRP conjugation enables enzymatic signal amplification, typically via secondary antibodies (e.g., ab97051 in Source ). While no direct HRP-conjugated LAMB1 primary antibodies are detailed in the sources, standard protocols involve pairing unconjugated LAMB1 antibodies with HRP-linked secondary reagents.
Cross-Reactivity and Applications
| Feature | Details | Source |
|---|---|---|
| Species Reactivity | Human, Mouse, Rat | |
| Applications | WB, IHC, ICC/IF, Flow Cytometry | |
| Dilution Range | WB: 1:500–1:5000; IHC: 1:50–1:500; ICC/IF: 1:100–1:500 |
Performance Data
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Western Blot: Detects bands at ~250 kDa (observed) vs. 198 kDa (predicted), suggesting post-translational modifications .
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Immunofluorescence: Cytoplasmic staining in HepG2 and NIH/3T3 cells .
HRP Conjugation Workflow
HRP-conjugated secondary antibodies (e.g., Goat Anti-Rabbit IgG-HRP) are used with primary LAMB1 antibodies for signal detection. Example protocol from Source :
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Primary Antibody: Anti-LAMB1 (1:100–1:5000 dilution).
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Secondary Antibody: HRP-conjugated IgG (1:100,000 dilution).
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Detection: Chemiluminescent substrate exposure (20–70 seconds).
Research Findings
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Developmental Roles: LAMB1 is essential for basement membrane integrity and cerebral cortex lamination .
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Disease Associations: Dysregulation linked to leukodystrophy and cancer metastasis .
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Apoptosis Markers: Caspase-mediated cleavage of laminin subunits (e.g., Lamin B1) serves as an apoptosis indicator .
Limitations
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
Laminin, binding to cells through a high-affinity receptor, is believed to mediate cell attachment, migration, and tissue organization during embryonic development. This occurs through interactions with other extracellular matrix components. Laminin is involved in establishing the laminar architecture of the cerebral cortex. It is essential for maintaining the integrity of the basement membrane/glia limitans, which serves as an anchor point for radial glial cell end-feet and a physical barrier to migrating neurons. Radial glial cells are crucial for cerebral cortical development, functioning as both the proliferative unit of the cortex and a scaffold for neuronal migration towards the pial surface.
LAMB1's Role in Disease and Cellular Processes: A Review of Key Findings
- High cytoplasmic LAMB1 expression correlates with oral squamous cell carcinomas. (PMID: 29479990)
- Laminin-1 or its YIGSR peptide (binding to 67LR) treatment of NS-1 cells reduces 67LR cell-surface expression and induces internalization. (PMID: 29108990)
- In vitro PDGFR-alpha activation leads to LAMB1 translational activation, inducing an invasive and metastatic phenotype in K19-expressing hepatocellular carcinoma cells. (PMID: 28783171)
- LAMB1 may influence pneumoconiosis initiation and progression, potentially serving as a diagnostic biomarker and indicator of genetic susceptibility. (PMID: 28444932)
- LAMB1 is a potential serological biomarker for colorectal cancer diagnosis, potentially used in conjunction with carcinoembryonic antigen. (PMID: 26359947)
- MUC5B production is regulated by ECM components; fibronectin and laminin upregulate MUC5B via integrin, ERK, and NF-κB pathways. (PMID: 26057585)
- LAMB1 rs2158836 polymorphisms are associated with symptom severity in Korean autism spectrum disorder patients. (PMID: 25774865)
- LAMB1 single nucleotide polymorphism rs886774 is associated with early-onset ulcerative colitis, suggesting distinct pathogenesis compared to Crohn's disease. (PMID: 25664710)
- LAMB1 expression upregulation correlates with miR-124-5p downregulation; miR-124-5p suppresses LAMB1 protein expression. (PMID: 24497408)
- The phenotype associated with LAMB2 mutations shows similar presentation across different ethnic groups. (PMID: 23679161)
- Homozygous deleterious mutations in LAMB1 have been identified. (PMID: 23472759)
- Elevated laminin beta1 and integrin alpha2 expression is observed in the anterior temporal neocortex of patients with intractable epilepsy. (PMID: 21370991)
- No association was found between rs6949033 (LAMB1) and ulcerative colitis. (PMID: 21744425)
- mRNA encoding laminin alpha1, beta1, and gamma1 chains is expressed in 90% of endometriotic lesions. (PMID: 12615822)
- LAMB1 expression alteration is linked to uterine leiomyoma genesis and development. (PMID: 15706419)
- Laminin beta1 chain (laminin-8 constituent) is found in carcinoma and metastasis vessel walls, but not in normal breast tissue. (PMID: 15987446)
- Laminin supports platelet adhesion through VWF and GPIb-IX-V interaction under high shear flow. (PMID: 18450753)
- Meningioma growth involves increased extracellular matrix production; neoplasia-associated cell proliferation requires significant extracellular matrix interaction. (PMID: 18474427)
- Elevated laminin beta1 mRNA and protein in the hippocampus suggests a role in intractable epilepsy seizure development. (PMID: 18691630)
- Beta2-laminins bind more strongly to alpha3beta1 and alpha7X2beta1 integrins than beta1-laminins. (PMID: 19147489)
- Nuclear beta-catenin and high MMP-9 levels in tumors correlate with abnormal cytoplasmic laminin accumulation; a feature of highly invasive and metastatic colorectal cancer. (PMID: 19526105)
- LAMB1 represents novel susceptibility loci for ulcerative colitis. (PMID: 19915572)
