LEM3 Antibody
Description
Definition of LEM3 Antibody
The Anti-LEMD3 Antibody (HPA025078) cataloged by Atlas Antibodies is a rabbit polyclonal antibody targeting human LEMD3 (LEM Domain-Containing Protein 3). LEMD3 is a nuclear envelope protein involved in TGF-β signaling regulation and musculoskeletal development .
Key Features of HPA025078:
| Property | Detail |
|---|---|
| Target Species | Human |
| Validation Methods | IHC, Western Blot (Enhanced) |
| Concentration | 0.2 mg/ml |
| Applications | Research use only |
Biological Context of LEM-3 vs. LEMD3
The term "LEM-3" in scientific literature (e.g., C. elegans studies) refers to LEM-3/Ankle1, a nuclease resolving chromatin bridges during cell division[1-4]. This protein is distinct from human LEMD3.
Functional Comparison:
Research Findings on LEM-3/Ankle1
While no direct data exists for a "LEM3 Antibody" targeting LEM-3/Ankle1, studies in C. elegans elucidate its critical roles:
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Chromatin Bridge Resolution: LEM-3 localizes to the midbody during cytokinesis, processing DNA bridges from replication errors or unresolved recombination intermediates .
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Meiotic Recombination: LEM-3 cooperates with MUS-81 and SLX-4 nucleases to resolve recombination intermediates, ensuring proper chromosome segregation .
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Regulation: AIR-2/Aurora B kinase modulates LEM-3’s midbody localization and activity .
Antibody Validation for LEMD3
The Anti-LEMD3 Antibody (HPA025078) has been validated for:
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Immunohistochemistry (IHC): Detects LEMD3 in human tissue sections.
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Western Blot (WB): Identifies ~75 kDa band corresponding to LEMD3 .
Limitations:
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No peer-reviewed studies using this antibody are cited in the provided sources.
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Cross-reactivity with other LEM-domain proteins remains unverified.
Implications and Gaps
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Nomenclature Clarity: "LEM3 Antibody" may cause confusion between LEM-3/Ankle1 (nuclease) and LEMD3 (nuclear envelope protein).
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Research Needs: Antibodies specific to LEM-3/Ankle1 are not described in the provided sources, highlighting a gap in tool availability for studying this nuclease.
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Studies involving the import of choline phospholipids into S. cerevisiae DeltaLem3 vector have demonstrated that human TMEM30a and Lem3p-TMEM30a chimeras can partially reconstitute this process. This finding suggests that these proteins are orthologous. PMID: 21289302
- The Lem3p-Dnf1p complex may have a distinct role beyond its plasma membrane aminophospholipid translocase activity when it substitutes for the Cdc50p-Drs2p complex, its redundant partner in the endosomal/trans-Golgi network compartments. PMID: 16600184
- Mutations in LEM3/ROS3 have been observed to impair growth in specific media, indicating that Lem3p is involved in the utilization of extracellular phosphatidylcholine (PC) with short acyl chains. PMID: 19270412
KEGG: sce:YNL323W
STRING: 4932.YNL323W
