At1g67640 Antibody

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Description

General Context: Arabidopsis Thaliana Antibodies

Antibodies targeting Arabidopsis proteins often follow standardized nomenclature, such as At1gXXXX, where "At1g" denotes a gene locus on chromosome 1. For example:

  • At1g53070 Antibody (CSB-PA108093XA01DOA): Targets a protein in Arabidopsis, used in Western blot (WB) and immunofluorescence (IF) .

  • At1g53060 Antibody (CSB-PA140131XA01DOA): Reacts with Arabidopsis proteins, applicable in WB and IF .

These antibodies are typically:

CharacteristicDetails
TypePolyclonal or monoclonal, often raised against recombinant proteins .
ApplicationsWB, IF, IP (immunoprecipitation), RIP (RNA immunoprecipitation) .
ReactivityArabidopsis thaliana (Mouse-ear cress) .
Molecular Weight~155 kDa (e.g., At1g53070) .

Hypothetical Profile of At1g67640 Antibody

Assuming "At1g67640 Antibody" follows conventions similar to other Arabidopsis antibodies:

ParameterInferred Details (Based on Analogous Antibodies)
Gene SymbolLikely a protein-coding gene in Arabidopsis thaliana (e.g., transporter, enzyme).
FunctionPotential role in plant stress response, membrane transport, or signaling (e.g., At1g53070: uncharacterized protein) .
Antibody ClassPolyclonal (common for plant-specific antibodies) .
Epitope SpecificityRaised against recombinant protein or peptide sequence .
Detection MethodsWB, IF, IP, RIP .

Research Gaps and Limitations

The absence of "At1g67640 Antibody" in indexed literature highlights:

  1. Niche Focus: Antibodies targeting specific Arabidopsis proteins may be limited to specialized studies (e.g., membrane transporters , stress responses ).

  2. Typographical Errors: Possible mismatches in gene nomenclature (e.g., "At1g67640" vs. "At1g53070").

  3. Emerging Research: The antibody may be newly developed or proprietary, with limited publication history.

Recommendations for Further Inquiry

To resolve ambiguity:

  1. Validate Gene Identifier: Confirm "At1g67640" via Arabidopsis genome databases (e.g., TAIR, UniProt).

  2. Explore Vendor Catalogs: Check suppliers like Proteintech, Cusabio, or Abcam for catalog entries.

  3. Review Literature: Search platforms like PubMed or Google Scholar using keywords:

    • "At1g67640 antibody Arabidopsis"

    • "Arabidopsis thaliana At1g67640 protein detection"

Critical Considerations in Antibody Design

For hypothetical development of At1g67640 Antibody:

  1. Epitope Selection: Prioritize regions with low homology to other plant proteins to minimize cross-reactivity .

  2. Validation: Use orthogonal methods (e.g., CRISPR knockout) to confirm target specificity .

  3. Applications: Optimize for techniques like WB (1:200–1:1000 dilution) or IF (1:500–1:1000) .

Product Specs

Buffer
Preservative: 0.03% Proclin 300
Composition: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Lead Time
Made-to-order (14-16 weeks)
Synonyms
At1g67640 antibody; F12A21.22 antibody; F12B7.20Lysine histidine transporter-like 2 antibody
Target Names
At1g67640
Uniprot No.

Target Background

Function
This antibody targets an amino acid transporter.
Database Links

KEGG: ath:AT1G67640

STRING: 3702.AT1G67640.1

UniGene: At.52423

Protein Families
Amino acid/polyamine transporter 2 family, Amino acid/auxin permease (AAAP) (TC 2.A.18.2) subfamily
Subcellular Location
Cell membrane; Multi-pass membrane protein.

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