liph Antibody

Structure and Function of LIPH

LIPH (Gene ID: 79858) is a membrane-associated enzyme that catalyzes the hydrolysis of phosphatidic acid to produce lysophosphatidic acid (LPA). It belongs to the AB hydrolase superfamily and contains a 12-residue lid region covering its catalytic pocket, along with four potential N-linked glycosylation sites . Defects in LIPH are associated with hypotrichosis type 7 (HYPT7), a congenital hair loss disorder .

Applications of LIPH Antibody

The LIPH antibody is validated for multiple experimental techniques:

Research Findings

LIPH has been implicated in cancer progression and metabolic regulation:

• Pancreatic Cancer: LIPH promotes glycolysis by stabilizing aldolase A (ALDOA) and activating the LPA/LPAR axis, supporting tumor growth. Its inhibition enhances sensitivity to gemcitabine-based therapies .

• Breast Cancer: Overexpression of LIPH correlates with metastasis and poor prognosis by enriching stem-like cells .

• Lung Adenocarcinoma: LIPH is selectively upregulated in bronchioloalveolar carcinomas, suggesting its role as a diagnostic biomarker .

Protocols and Validation

• Western Blot: Use 1:500–1:2000 dilution with HRP-conjugated secondary antibodies. Blocking buffer: 3% nonfat dry milk in TBST .

• IHC: Antigen retrieval with TE buffer (pH 9.0) or citrate buffer (pH 6.0) is recommended .

• Immunoprecipitation: Use 0.5–4.0 µg antibody per 1–3 mg lysate .