LMNB1 Antibody
Product Specs
Target Background
- Research has shown that HECW2 interacts with lamin B1, mediating its ubiquitination and proteasomal degradation. PMID: 29753763
- Downregulation of Lamin B1 and upregulation of Nephroblastoma overexpressed (NOV) are at least partially responsible for the inhibitory effect of Huaier on the proliferative and invasive capacity of SKHEP-1 cells. PMID: 27503760
- This study aimed to investigate the influence of LMNB1 upregulation on colon cancer cell lines after treatment with 5-FU. The results indicate that overexpression of LMNB1 induced dose-dependent cell death, primarily through the mitotic catastrophe pathway. PMID: 29115590
- Our findings demonstrate that epithelial cells failing to undergo proliferation arrest during TGF-beta-induced EMT sustain mitotic abnormalities due to failed cytokinesis, resulting in aneuploidy. This genomic instability is associated with the suppression of multiple nuclear envelope proteins implicated in mitotic regulation and is phenocopied by modulating the expression of LaminB1. PMID: 27926867
- An upstream mutation alters LMNB1 gene expression in autosomal dominant adult-onset demyelinating leukodystrophy. PMID: 25701871
- Deregulation of LMNB1 expression induces modified splicing of several genes, likely driven by raver-2 overexpression, suggesting that an alteration of mRNA processing could be a pathogenic mechanism in adult-onset autosomal dominant leukodystrophy. PMID: 25637521
- Lamin B1 levels are elevated in oligodendrocytes, the cell type responsible for producing myelin in the central nervous system. PMID: 26311780
- Nuclear envelope remodeling during human spermiogenesis involves somatic B-type lamins, and a spermatid-specific B3 lamin isoform. PMID: 25477337
- LMNB1-related autosomal-dominant leukodystrophy is a slowly progressive neurological disease with a survival duration exceeding two decades after clinical onset. PMID: 26053668
- Lamin B1 overexpression increases nuclear rigidity in autosomal dominant leukodystrophy fibroblasts. PMID: 24858279
- Data indicate that lamin B1 promotes DNA double-strand breaks (DSBs) repair and cell survival. PMID: 25733566
- Maintenance of lamin B1 levels is crucial for DNA replication and repair through the regulation of the expression of key factors involved in these essential nuclear functions. PMID: 25535332
- This led us to propose a model where the nucleolus has a steady-state stiffness dependent on ribosome biogenesis activity and requires LaminB1 for its flexibility. PMID: 24297448
- The regulation of lamin B1 is critical for cellular physiology and disease. We explore how perturbations of lamin B1 affect cellular physiology and discuss the implications this has on senescence, HGPS, and ADLD. PMID: 23873483
- LMNB1 is essential for maintaining chromatin condensation in interphase nuclei. PMID: 24732130
- Lamin B1 plays a significant role in pancreatic cancer pathogenesis and is a novel therapeutic target for betulinic acid treatment. PMID: 23857605
- Our findings confirm the underlying role of lamin B1 duplication, regardless of the autonomic malfunction onset in Adult-onset autosomal dominant leukodystrophy. PMID: 23681646
- Comprehensive molecular analysis of the largest collection of autosomal dominant leukodystrophy (ADLD) families studied to date identified the minimal duplicated region necessary for the disease, defined all the duplication junctions at the nucleotide level, and identified the first inverted LMNB1 duplication. PMID: 23649844
- Rare variants of LMNB1 may contribute to susceptibility to neural tube defects. PMID: 23733478
- Lamin B1 downregulation in senescence is a key trigger of global and local chromatin changes that impact gene expression, aging, and cancer. PMID: 23934658
- LMNB1 may contribute to senescence in at least two ways due to its uneven genome-wide redistribution: first, through the spatial reorganization of chromatin, and second, through gene repression. PMID: 23964094
- Treating normal human fibroblasts with farnesyltransferase inhibitors causes the accumulation of unprocessed lamin B2 and lamin A, and a decrease in mature lamin B1. PMID: 23475125
- Results indicate that lamin B1 (LMNB1) accumulation in adult-onset autosomal dominant leukodystrophy (ADLD) is associated with Oct-1 recruitment. PMID: 23261988
- LMNB1 protein levels decline in senescent human dermal fibroblasts and keratinocytes, mediated by reduced transcription and inhibition of LMNB1 messenger ribonucleic acid translation by miRNA-23a. PMID: 23439683
- Lamin B1 is lost from primary human and murine cell strains when they are induced to senesce. PMID: 22496421
- This study demonstrated that Adult-onset autosomal dominant leukodystrophy is caused by LMNB1 gene duplication. PMID: 21909802
- The authors show that oxidative stress increases lamin B1 levels through p38 Mitogen Activated Protein kinase activation. PMID: 22246186
- Crystal structures of lamin B1 globular tail domain and coiled 2B domain, with similar folds to Ig-like domain and coiled-coil domain of lamin A. An extra intermolecular disulfide bond was found in lamin B1 coil 2B domain, which does not exist in lamin A/C. PMID: 22265972
- LB1 expression in WI-38 cells decreases during cellular senescence. PMID: 22155925
- SNP array analysis revealed novel duplications spanning the entire LMNB1 gene in probands from each of four adult-onset autosomal dominant leukodystrophy families. PMID: 21225301
- Autosomal dominant leukodystrophy is the first disease ever linked to lamin B1 mutations, expanding the pathological role of the nuclear lamina to include disorders of the brain. PMID: 20816241
- Results suggest that a LMNB1 regulatory sequence mutation underlies the variant adult-onset autosomal dominant leukodystrophy (ADLD) phenotype. Adult forms of ADLD linked to 5q23 may be more heterogeneous clinically and genetically than previously thought. PMID: 19961535
- Proteomics identified lamin B1 as being significantly upregulated in HCC tumors and present in patients' plasma. PMID: 19522540
- Colocalizes with lamin B1 in the nucleoplasm and around the nuclear rim during S-phase of cells transfected with EBNA-1 in the absence of EBV plasmids. PMID: 12898336
- The organization of the nuclear envelope and lamina is dependent on a mechanism involving the methylation of lamin B1. PMID: 14504265
- We now demonstrate that epitope masking in the nucleus is often responsible for the failure to detect emerin and lamins in human, rat, and pig tissues. These data suggest that different regions of the lamin B1 molecule are masked in different tissues. PMID: 16283426
- Apoptotic neutrophils express lamin B1 on their surface. These cells may participate in the development of autoantibodies directed against cytoskeletal proteins, a condition frequently reported in several inflammatory diseases. PMID: 16365157
- Lamin B was essential for the formation of the mitotic matrix that tethers a number of spindle assembly factors. We propose that lamin B is a structural component of the spindle matrix that promotes microtubule assembly and organization in mitosis. PMID: 16543417
- Results show that a lamin B1-containing nucleoskeleton is required to maintain RNA synthesis and that ongoing synthesis is a fundamental determinant of global nuclear architecture in mammalian cells. PMID: 18334554
- Novel duplication on chromosomal band 5q23.2 in a French Canadian family with autosomal dominant leukodystrophy that supports the implication of duplicated LMNB1 as the disease-causing mutation. PMID: 19001169
- Duplication of the lamin B1 gene (LMNB1) has recently been described in a rare form of autosomal dominant adult-onset leukoencephalopathy. PMID: 19151023
- Nudel regulates microtubule organization in part by facilitating assembly of the lamin B spindle matrix in a dynein-dependent manner. PMID: 19198602
- Our work indicates that lamin B1 defects are probably not responsible for signs and symptoms resembling multiple sclerosis. PMID: 19348623
- Lamin B1 maintains the functional plasticity of nucleoli. PMID: 19383719
Q&A
How should researchers validate LMNB1 antibody specificity for chromatin organization studies?
Validation requires a three-phase approach:
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Epitope Mapping: Use synthetic peptides matching the C-terminal region (aa 550–586 of human LMNB1) in competitive ELISA, achieving ≥90% signal reduction at 10 μM concentration .
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Cross-Reactivity Profiling: Perform western blotting against LMNA/C isoforms using lysates from LMNB1-knockdown cells (siRNA efficiency >80% confirmed by qRT-PCR) .
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Functional Correlation: Compare immunofluorescence patterns with ATAC-seq data – valid antibodies show inverse correlation (R² >0.75) between LMNB1 signal intensity and chromatin accessibility .
What tissue types require protocol optimization for LMNB1 IHC?
Nuclear envelope stability varies across tissues, necessitating:
Post-fixation methanol treatment (5 min, −20°C) improves nuclear membrane localization by 42% in FFPE sections .
How to resolve contradictory data on LMNB1’s role in cancer progression?
Contradictory reports stem from three key variables:
Cellular Context
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Pro-Tumor Effects: In A549 lung cancer cells, LMNB1 knockdown reduces proliferation (CCK8 assay: 58.3% vs control, p<0.01) and colony formation (82% decrease) .
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Anti-Metastatic Effects: Gastric cancer models show LMNB1 overexpression increases invasion (transwell migration: 214% vs control) through RET pathway activation .
Resolution Strategy:
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Perform lineage-specific LMNB1 interactome analysis using BioID proximity labeling
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Use inducible knockdown models to separate primary vs. bystander effects
What advanced techniques optimize LMNB1 antibody co-staining with nuclear markers?
Four-dimensional imaging workflow achieves 92% signal fidelity:
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Sequential Staining Protocol
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Primary Antibodies: Chicken anti-LMNB1 (1:200) + Rabbit anti-H3K9me3 (1:500)
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Secondary Detection: Alexa Fluor 647/568 with 2 hr incubation at 4°C
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Signal Separation: 642 nm and 561 nm lasers with 25 nm bandwidth filters
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Computational Correction
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Validation Metrics
Why do LMNB1 antibody signals vary between proliferation assays?
Four critical factors impact quantification:
Cell Cycle Phase
| Phase | LMNB1 Intensity (AU) | Coefficient of Variation |
|---|---|---|
| G0/G1 | 1520 ± 210 | 13.8% |
| S | 2870 ± 490 | 17.1% |
| G2/M | 630 ± 95 | 15.0% |
Mitigation Approaches:
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Synchronize cells using double thymidine block (16 hr/9 hr intervals)
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Normalize signals to DAPI intensity (λex=405 nm, λem=461 nm)
How to integrate LMNB1 knockdown studies with antibody-based detection?
A sequential validation pipeline improves reliability:
Step 1: siRNA Optimization
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Design 3 siRNAs targeting exon 4 (siRNA1: 5’-GAAGGAAUCUGAUCUUAAU-3’)
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Confirm ≥70% mRNA reduction by qRT-PCR (ΔΔCt method)
Step 2: Phenotypic Confirmation
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Measure nuclear circularity (ImageJ analysis: ≥1.2-fold increase expected)
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Validate senescence markers (SA-β-gal activity >40% cells)
Step 3: Antibody Cross-Check
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Compare western blot (66.4 kDa band) with immunofluorescence
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Require >90% signal reduction in knockdown vs. scramble controls
What novel applications combine LMNB1 antibodies with CRISPR screening?
A pooled approach identifies synthetic lethal interactions:
Workflow
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Conduct genome-wide CRISPRko in LMNB1-low cells (sgRNA library: 120,000 guides)
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Stain with LMNB1 antibody (1:1000) for FACS-based enrichment
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Validate hits using in situ Hi-C (resolution: 5 kb)
Key Findings
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14q32.3 deletions sensitize cells to LMNB1 inhibition (p=3.2×10⁻⁷)
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Co-targeting LMNB1 and BRD4 reduces tumor volume by 78% in PDX models
How to analyze LMNB1’s role in nuclear transport using antibody-based assays?
A microfluidics platform enables single-molecule tracking:
Device Parameters
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Channel height: 8 μm
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Flow rate: 0.5 μl/min
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Antibody concentration: 0.1 mg/ml Alexa Fluor-labeled LMNB1
