LPXD1 Antibody

Validation Data

• Western Blot (WB): Detected in MDA-MB-231, PC-3, and RAW 264.7 cells .

• Immunofluorescence (IF): Confirmed in A549 cells .

Functional Insights

• Role in Cancer: LPXN contributes to metastasis by promoting integrin-mediated cell migration in cancers such as breast and prostate .

• Mechanistic Studies: Used to investigate LPXN’s interaction with paxillin and focal adhesion kinase (FAK) in cytoskeletal reorganization .

Key Findings

• LPXN acts as a negative regulator of integrin-mediated adhesion, influencing cellular responses to extracellular matrix signals .

• In Leptospira interrogans, the lpxD1 gene (unrelated to LPXN) is under positive selection for thermal adaptation and membrane integrity .

Comparative Analysis: LPXN vs. Bacterial lpxD1

Antibody Performance and Best Practices

• Dilution Guidelines:

  • WB: Optimize between 1:500–1:3000.

  • IF/ICC: Use 1:200–1:800 for clear signal-to-noise ratios .

• Controls: Include recombinant LPXN protein or siRNA-mediated knockdown samples for validation.

Broader Context: Antibody Development Trends

While LPXN antibodies are established tools, recent advances in monoclonal antibody (mAb) discovery highlight challenges in targeting membrane proteins (e.g., E. coli LptD) and optimizing Fc engineering for reduced immunogenicity (e.g., anti-PD-1 antibodies) .