LECRK43 Antibody
Description
Definition and Biological Context
LECRK43 (L-type Lectin-Domain Containing Receptor Kinase IV.3) is a plant-specific protein encoded in Arabidopsis thaliana (Mouse-ear cress). It belongs to the lectin receptor-like kinase (LecRLK) family, which combines carbohydrate-binding lectin domains with intracellular kinase signaling domains. These proteins are critical for plant immune responses, cell wall interactions, and stress signaling .
The LECRK43 antibody is a custom-developed reagent designed to detect and study this protein in experimental settings. While commercial availability is limited, specialized providers like Cusabio offer custom antibody services for plant biology research .
Protein Structure and Functional Domains
LECRK43 features two key domains:
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Extracellular lectin domain: Binds carbohydrate moieties, potentially involved in pathogen recognition.
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Intracellular serine/threonine kinase domain: Mediates downstream signaling cascades.
| Feature | Details |
|---|---|
| UniProt ID | O81292 |
| Gene | LECRK43 (AT1G69920) |
| Molecular Weight | ~70 kDa (predicted) |
| Species | Arabidopsis thaliana |
| Tissue Expression | Root, shoot, and floral tissues (predicted based on homologs) |
Research Applications
LECRK43 antibodies are primarily used in:
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Immunolocalization: Tracking protein expression in plant tissues.
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Western blotting: Confirming protein presence in lysates.
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Functional studies: Investigating roles in pathogen defense and stress responses.
Key findings from related LecRLK studies :
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Lectin receptor kinases in Arabidopsis regulate symbiotic interactions and immune responses.
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Homologs of LECRK43 (e.g., LECRK-I.9) are implicated in modulating salt stress tolerance.
Challenges and Limitations
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Specificity: Cross-reactivity with other lectin-domain proteins is a common issue .
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Commercial scarcity: Few validated antibodies exist, necessitating custom development .
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Functional data gaps: Direct evidence linking LECRK43 to specific pathways remains sparse.
Future Directions
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Structural studies: Resolving the lectin domain’s carbohydrate-binding specificity.
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CRISPR/Cas9 knockouts: Elucidating phenotypic impacts in Arabidopsis.
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Cross-species analysis: Exploring homologs in crops for agricultural applications.
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- AtLPK1 plays essential roles in both abiotic and biotic stress responses in *Arabidopsis thaliana*. PMID: 23415333
Q&A
Here’s a structured collection of FAQs tailored for researchers studying LECRK43 Antibody, designed to address both foundational and advanced scientific inquiries:
Advanced Research Questions
How can I resolve contradictory data on LECRK43’s role in [specific pathway]?
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Analysis framework:
What strategies improve LECRK43 Antibody efficacy in in vivo models?
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Approach:
How do I design a study to explore LECRK43’s interaction network?
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Workflow:
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Use co-immunoprecipitation (Co-IP) with LECRK43 Antibody followed by mass spectrometry.
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Apply BioID proximity labeling to map transient interactions.
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Validate hits via knockdown/overexpression + functional assays (e.g., migration, proliferation).
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Data Contradiction Analysis
Why does LECRK43 Antibody show differential staining in tumor vs. normal tissues?
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Potential factors:
Factor Investigative Action Epitope masking Antigen retrieval optimization Post-translational modifications Phosphatase/protease treatment Off-target binding Cross-validate with RNAscope
How to interpret conflicting results between ELISA and IHC?
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Troubleshooting:
