LECRK55 Antibody
Description
Molecular Target: LECRK55 Protein
LECRK55 is a transmembrane receptor kinase with an extracellular L-type lectin domain. It confers resistance to oomycete pathogens like Phytophthora infestans and Phytophthora capsici but increases susceptibility to bacterial pathogens . The protein is implicated in modulating plant immune responses through pathogen recognition and signal transduction .
Antibody Development and Characteristics
The LECRK55 Antibody (Product Code: CSB-PA853339XA01DOA) is a polyclonal antibody developed using recombinant Arabidopsis thaliana LECRK55 protein as the immunogen. Key specifications include :
| Property | Detail |
|---|---|
| Host Species | Rabbit |
| Reactivity | Arabidopsis thaliana |
| Applications | ELISA, Western Blot (WB) |
| Clonality | Polyclonal |
| Purification Method | Antigen Affinity Purified |
| Storage | -20°C or -80°C; avoid repeated freeze-thaw cycles |
| Formulation | Liquid (50% glycerol, 0.01M PBS, pH 7.4, 0.03% Proclin 300 preservative) |
Research Applications
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Pathogen Interaction Studies: Used to investigate LECRK55’s role in plant defense against oomycetes .
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Protein Localization: Enables tracking of LECRK55 expression in plant tissues under pathogen challenge.
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Mechanistic Insights: Facilitates studies on lectin-mediated signaling pathways and their interplay with microbial effectors .
Technical Validation
The antibody has been validated for:
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Specificity: Binds exclusively to the recombinant LECRK55 protein in Arabidopsis thaliana .
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Sensitivity: Effective in detecting low-abundance LECRK55 in complex plant extracts via WB .
Significance in Plant Immunity
LECRK55 is part of a broader family of lectin receptor kinases (LecRKs) that regulate plant-microbe interactions. Its dual role in resistance and susceptibility highlights the complexity of plant immune systems . The antibody’s ability to target this protein aids in:
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Mapping signaling cascades triggered by pathogen-associated molecular patterns (PAMPs).
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Identifying cross-talk between lectin domains and other immune receptors.
Future Directions
Current research leveraging this antibody focuses on:
Product Specs
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- LecRK-V.5 negatively regulates stomatal immunity upstream of reactive oxygen species (ROS) biosynthesis. PMID: 22346749
- LecRK-V.5 represses resistance to necrotrophic *Pcc* bacteria, potentially through its effects on stomatal immunity. [LecRK-V.5] PMID: 22899085
