LSM5 Human

LSM5 Homolog, U6 Small Nuclear RNA Associated Human Recombinant
Shipped with Ice Packs
In Stock
Cat. No.
BT20880
Source
Escherichia Coli.
Synonyms
LSM5 homolog, U6 small nuclear RNA associated (S.cerevisiae), YER146W, U6 snRNA-associated Sm-like protein LSm5.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Quick Inquiry

Description

Introduction and Biochemical Characteristics

LSM5 Human is a 12.3 kDa protein encoded by the LSM5 gene (chromosome 7 in humans) and belongs to the Sm-like protein family. It consists of 91 amino acids (1–91 residues) and shares sequence homology with Sm proteins, which form stable heteromers in tri-snRNP complexes .

Key Biochemical Features

PropertyDetails
Amino Acid SequenceIncludes conserved Sm motifs (e.g., residues 1–91)
Molecular Weight12.3 kDa (non-glycosylated)
Expression HostRecombinant production in E. coli with N-terminal His-tag
Purity>95% (SDS-PAGE validated)
Formulation20 mM Tris-HCl (pH 8.5), 0.15 M NaCl, 1 mM DTT, 10% glycerol

Spliceosome Assembly and Pre-mRNA Splicing

LSM5 is integral to the U4/U6-U5 tri-snRNP complex, stabilizing U6 snRNA and enabling spliceosome formation . The LSM2-8 complex binds the U6 snRNA 3'-terminal U-tract, ensuring proper splicing .

Circadian Rhythm Regulation

LSM5 influences circadian period length in plants and mammals by modulating core clock gene expression (e.g., CCA1, LHY, TOC1) and alternative splicing . Mutations in LSM5 or LSM4 in Arabidopsis delay midday gene expression and extend circadian periods .

Tissue Expression and Cellular Localization

LSM5 is ubiquitously expressed, with high levels in:

Tissue/OrganExpression LevelSource
Brain (Cerebral Cortex)High
LiverModerate
KidneyModerate
ColonHigh (Tumor vs. Normal)

Nuclear localization is critical for its role in splicing, while cytoplasmic LSM complexes regulate mRNA decay .

Overexpression and Prognostic Impact

LSM5 is upregulated in multiple cancers, correlating with poor survival:

Cancer TypeObservationReference
Colon CancerHigh LSM5 expression linked to reduced survival (69% vs. 43%)
Gastric CancerAssociated with chemoresistance and immune evasion
Breast CancerOverexpression tied to aggressive subtypes (e.g., HER2+)
Hepatocellular CarcinomaPart of a 4-gene risk score predicting poor OS

Mechanistic Insights

  • Colon Cancer: LSM5 knockdown inhibits proliferation and upregulates p53, CDKN1A, and TNFRSF10B, inducing apoptosis .

  • Gastric Cancer: LSM5 correlates with reduced T-cell infiltration and chemoresistance to 5-FU .

Experimental Data

  • Gene Knockdown:

    • Colon Cells: Reduced colony formation (MTT assay IC₅₀: ~50% inhibition) .

    • Hepatocellular Cells: LASSO regression identifies LSM5 as a key prognostic marker .

  • Gene Expression Profiling:

    • Gastric Cancer: LSM5 overexpression in TCGA/ICGC cohorts correlates with advanced stages .

Therapeutic Targets

LSM5 inhibition may block tumor growth in colon and gastric cancers by restoring p53 signaling and enhancing immune recognition .

Product Specs

Introduction
LSM5, also known as LSM5 Homolog, U6 Small Nuclear RNA Associated, is a member of the Sm protein family. This family is found in various organisms and is characterized by the presence of the Sm sequence motif. The Sm motif comprises two regions separated by a variable-length linker that forms a loop. Sm-like proteins are believed to assemble into a stable heteromer within tri-snRNP particles, which play a crucial role in pre-mRNA splicing. LSM5 has been associated with diseases like spinal muscular atrophy and muscular atrophy.
Description
Recombinant Human LSM5 is produced in E. coli. It is a single, non-glycosylated polypeptide chain consisting of 114 amino acids (1-91 a.a) with a molecular weight of 12.3 kDa. The protein includes a 23 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
LSM5 protein solution at a concentration of 1mg/ml in a buffer containing 20mM Tris-HCl (pH 8.5), 10% glycerol, 0.15M NaCl, and 1mM DTT.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For longer storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is greater than 95.0% as determined by SDS-PAGE analysis.
Synonyms
LSM5 homolog, U6 small nuclear RNA associated (S.cerevisiae), YER146W, U6 snRNA-associated Sm-like protein LSm5.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAANATT NPSQLLPLEL VDKCIGSRIH IVMKSDKEIV GTLLGFDDFV NMVLEDVTEF EITPEGRRIT KLDQILLNGN NITMLVPGGE GPEV

Q&A

Experimental Design for Studying LSM5 in Human Cells

  • Question: How should I design an experiment to study the role of LSM5 in human cells?

  • Answer: To study LSM5 in human cells, follow these steps:

    • Hypothesis Formation: Formulate a hypothesis regarding the function or impact of LSM5 on cellular processes.

    • Variable Selection: Identify independent variables (e.g., LSM5 expression levels) and dependent variables (e.g., cell proliferation, apoptosis).

    • Experimental Treatment: Design treatments to manipulate LSM5 expression (e.g., knockdown, overexpression).

    • Group Assignment: Use a between-subjects design with control and treatment groups.

    • Measurement: Quantify dependent variables using techniques like Western blotting or flow cytometry .

Data Analysis for LSM5 Expression Studies

  • Question: How can I analyze data from LSM5 expression studies to identify patterns or contradictions?

  • Answer: Use statistical methods to analyze LSM5 expression data:

    • Descriptive Statistics: Calculate means and standard deviations to summarize data.

    • Inferential Statistics: Apply t-tests or ANOVA to compare expression levels between groups.

    • Correlation Analysis: Use Pearson's r to assess relationships between LSM5 expression and other variables .

LSM5 and Cancer Research

  • Question: What role does LSM5 play in cancer, and how can it be studied?

  • Answer: LSM5 has been implicated in cancer progression and chemoresistance. To study its role:

    • Literature Review: Examine existing studies on LSM5 in cancer types like nasopharyngeal carcinoma and gastric cancer .

    • Experimental Models: Use cell lines and animal models to investigate LSM5's effects on tumor growth and drug sensitivity.

    • Bioinformatics Tools: Utilize databases like KM Plotter for survival analysis and TIMER for immune infiltration analysis .

LSM5 in Gliomas

  • Question: How does LSM5 contribute to glioma progression, and what are its diagnostic and therapeutic values?

  • Answer: LSM5 has been studied for its diagnostic and therapeutic potential in gliomas:

    • Expression Analysis: Investigate LSM5 expression levels in glioma samples using techniques like qRT-PCR.

    • Clinical Correlation: Analyze correlations between LSM5 expression and patient outcomes.

    • Therapeutic Strategies: Explore targeting LSM5 as a potential therapeutic approach for gliomas .

Methodological Considerations for LSM5 Research

  • Question: What methodological considerations should be taken into account when researching LSM5?

  • Answer: Key considerations include:

    • Sample Size and Power: Ensure sufficient sample sizes to achieve statistical power.

    • Control Groups: Include appropriate control groups for comparison.

    • Data Validation: Validate findings using multiple experimental approaches and datasets .

LSM5 and Immune Cell Infiltration

  • Question: How does LSM5 influence immune cell infiltration in tumors?

  • Answer: LSM5's impact on immune cell infiltration can be studied using:

    • Bioinformatics Tools: Utilize tools like TIMER to analyze correlations between LSM5 expression and immune cell infiltration.

    • Experimental Validation: Validate findings using techniques like immunohistochemistry (IHC) .

LSM5 as a Biomarker

  • Question: Can LSM5 serve as a biomarker for disease diagnosis or prognosis?

  • Answer: LSM5's potential as a biomarker depends on its expression patterns and correlations with disease outcomes:

    • Expression Profiling: Conduct comprehensive expression profiling across different disease states.

    • Clinical Validation: Validate LSM5's predictive value in clinical cohorts .

LSM5 and Chemotherapy Resistance

  • Question: How does LSM5 contribute to chemotherapy resistance in cancer cells?

  • Answer: LSM5's role in chemotherapy resistance can be investigated by:

    • Gene Expression Analysis: Analyze LSM5 expression in response to chemotherapy.

    • Functional Assays: Use assays like drug sensitivity tests to assess resistance mechanisms .

LSM5 in Different Cancer Types

  • Question: How does LSM5's role vary across different types of cancer?

  • Answer: LSM5's function can differ based on cancer type:

    • Literature Review: Compare studies across various cancers to identify patterns.

    • Experimental Comparison: Conduct experiments to compare LSM5's effects in different cancer cell lines .

Example Data Table: Correlation Between LSM5 Expression and Immune Cell Infiltration

LSM5 Expression LevelCD8+ T Cell InfiltrationB Cell Infiltration
HighLowLow
MediumMediumMedium
LowHighHigh

This table illustrates hypothetical correlations between LSM5 expression and immune cell infiltration, which can be analyzed using tools like TIMER .

Product Science Overview

Introduction

LSM5, also known as LSM5 Homolog, U6 Small Nuclear RNA Associated, is a protein coding gene that plays a crucial role in RNA metabolism. This gene is part of the Sm-like (LSM) protein family, which is identified in a variety of organisms based on sequence homology with the Sm protein family .

Structure and Function

LSM5 contains the Sm sequence motif, which consists of two regions separated by a linker of variable length that folds as a loop . The Sm-like proteins, including LSM5, are thought to form a stable heteromer present in tri-snRNP particles, which are important for pre-mRNA splicing . Specifically, LSM5 is a component of the U4/U6-U5 tri-snRNP complex involved in spliceosome assembly and the precatalytic spliceosome (spliceosome B complex) .

Biological Role

The heptameric LSM2-8 complex, which includes LSM5, binds specifically to the 3’-terminal U-tract of U6 snRNA . This binding is essential for the proper functioning of the spliceosome, a complex responsible for the removal of introns from pre-mRNA . Additionally, LSM5 is involved in mRNA decapping and degradation, further highlighting its role in RNA processing .

Associated Pathways

LSM5 is associated with several critical pathways, including the processing of capped intron-containing pre-mRNA and deadenylation-dependent mRNA decay . These pathways are vital for the regulation of gene expression and the maintenance of cellular homeostasis.

Clinical Significance

Mutations or dysregulation of LSM5 have been linked to certain diseases, such as spinal muscular atrophy . Understanding the function and regulation of LSM5 can provide insights into the molecular mechanisms underlying these conditions and potentially lead to the development of targeted therapies.

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

LSM5 Antibody
LSM5 Homolog, U6 Small Nuclear RNA Associated, Mouse Anti Human
Cat. No.
BT23986
Source
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.