LYK5 Antibody
Description
Validated Applications and Dilutions
LYK5 Antibody is optimized for multiple immunoassays, with dilution recommendations varying by supplier:
Supplier Comparison
LYK5 Antibodies are available from multiple manufacturers, each with distinct catalog numbers and reactivity profiles:
| Supplier | Catalog Number | Host | Reactivity | Key Applications | Dilution (WB) |
|---|---|---|---|---|---|
| Novus Biologicals | NBP1-89577 | Rabbit | Human | IHC, IF, IHC-Paraffin | 1:10,000 |
| FabGennix | STRAD-101AP | Rabbit | Human, Monkey | ELISA, IHC, IP, WB | 1:500 |
| Thermo Fisher | PA5-115978 | Rabbit | Human, Mouse | WB (endogenous STRAD) | 1:1,000–1:5,000 |
Notes:
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Novus and Fisher Scientific offer identical products under catalog numbers 103279-624 and NBP1-89577 ( ).
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Thermo Fisher’s antibody detects both human and mouse LYK5, expanding cross-species utility ( ).
Research Findings and Functional Insights
While the antibody itself is not directly implicated in functional studies, its role in detecting LYK5 enables investigations into its biological relevance:
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Subcellular Localization: LYK5 is primarily cytoplasmic and nuclear, as inferred from its interaction with LKB1/STK11 and roles in kinase activation ( ).
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Protein Interactions: LYK5 binds CAB39/MO25 proteins to activate LKB1, a tumor suppressor kinase involved in cellular energy sensing and apoptosis ( ).
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Cancer Relevance: Originally identified as a serologically defined breast cancer antigen (NY-BR-96), LYK5’s expression in liver tissues suggests potential biomarker utility ( ).
Critical Considerations
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Cross-reactivity: Verify species specificity, as some antibodies (e.g., FabGennix) target primate samples, while others (Novus, Thermo Fisher) focus on human/mouse systems.
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Application Optimization: Dilutions vary significantly between suppliers; follow recommended protocols for consistent results.
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Storage Stability: Avoid freeze-thaw cycles to maintain antibody integrity ( ).
