AtMg00050 Antibody
Description
Contextual Analysis of the AtMg00050 Designation
The "AtMg" prefix follows Arabidopsis thaliana mitochondrial gene nomenclature (e.g., AtMg00100 encodes a subunit of NADH dehydrogenase). While no entry for AtMg00050 exists in public genome databases (TAIR, NCBI, or UniProt), the identifier may correspond to an uncharacterized or deprecated gene locus. Antibodies targeting mitochondrial proteins in plants often focus on membrane transporters, tRNA import machinery, or respiratory chain components, as seen in studies of Tric1/Tric2 proteins .
Antibody Applications in Plant Mitochondrial Research
Key antibodies used in Arabidopsis mitochondrial studies include:
Antibody Validation Techniques
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Protease Sensitivity Assays: Differentiated integral membrane proteins (e.g., Tric1/2) from peripheral or matrix proteins .
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Co-immunoprecipitation: Demonstrated physical interaction between Tric1/2 and Tom40 in mitochondrial outer membrane complexes .
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Subcellular Localization: Immunogold labeling and confocal microscopy confirmed mitochondrial targeting .
Key Experimental Data
Table 1: Protease Sensitivity of Mitochondrial Proteins
| Protein | Protease (Concentration) | Degradation Pattern | Localization Inference |
|---|---|---|---|
| Tric1/2 | 0.4 µg Proteinase K | Partial cleavage (~26 kDa fragment stable) | Integral outer membrane protein |
| Tom40 | 2 µg Trypsin | Complete degradation | Cytosol-facing domain |
| Rieske Fe-S | 10 µg Proteinase K | No degradation | Inner membrane/matrix |
Hypothetical Framework for AtMg00050 Antibody
If AtMg00050 corresponds to a mitochondrial gene product, its antibody would likely follow similar validation protocols:
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Epitope Identification: Peptide synthesis based on predicted protein sequence.
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Specificity Testing: Western blotting against mitochondrial lysates and knockout lines.
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Functional Assays: Co-localization with Tom40 or Tric1/2 via immunofluorescence.
Gaps and Limitations
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No primary literature or commercial catalogs list "AtMg00050 Antibody."
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Mitochondrial gene annotation in Arabidopsis remains incomplete for hypothetical ORFs.
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Cross-reactivity risks (e.g., with chloroplast proteins) necessitate rigorous validation .
Recommendations for Future Research
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Perform homology modeling to predict AtMg00050 protein structure.
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Generate polyclonal antibodies using peptide antigens from conserved domains.
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Utilize CRISPR-Cas9 knockout lines to confirm antibody specificity.
Product Specs
Constituents: 50% Glycerol, 0.01M Phosphate Buffered Saline (PBS), pH 7.4
Target Background
KEGG: ath:ArthMp005
STRING: 3702.ATMG00050.1
