AtMg00400 Antibody
Description
Mitochondrial Protein Localization
In a 2016 study, the AtMg00400 antibody was used to investigate mitochondrial outer membrane (OM) proteins in Arabidopsis. Researchers confirmed its specificity by testing recombinant proteins and performing protease sensitivity assays . Key findings include:
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Protease Sensitivity: AtMg00400 exhibited partial resistance to Proteinase K (PK) and trypsin, suggesting a membrane-embedded topology similar to integral OM proteins like Tom40 .
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Interaction with TOM Complex: Co-immunoprecipitation assays revealed associations between AtMg00400 and components of the Translocase of the Outer Mitochondrial membrane (TOM) complex, indicating its role in mitochondrial protein import .
tRNA Import Mechanisms
The antibody helped identify AtMg00400’s involvement in tRNA translocation into mitochondria, a process critical for organellar translation and respiration. Disruption of AtMg00400 impaired tRNA accumulation in mitochondria, linking it to plant growth defects .
Future Directions
Further studies could explore AtMg00400’s role in stress responses or its interaction with other mitochondrial transporters. Comparative analyses across plant species may reveal evolutionary conservation of its function.
Product Specs
**Constituents:** 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
KEGG: ath:ArthMp034
STRING: 3702.ATMG00400.1
Q&A
The term "AtMg00400 Antibody" does not appear in any of the provided research materials or public antibody databases. The closest matching reference ( ) lists "AtMg00400.1" as an unannotated numerical identifier in a thesis appendix without experimental context, suggesting either a typographical error, discontinued nomenclature, or a highly specialized plant biology target not covered in the provided clinical/medical antibody research corpus.
Given this discrepancy, I propose restructured FAQs based on general antibody research methodologies demonstrated in the provided papers, which can serve as a template for investigating novel antibodies like AtMg00400:
Advanced Technical Challenges
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Resolving contradictory binding vs functional activity data (e.g., ELISA-positive but neutralization-negative):
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Optimizing antibody developability without compromising activity:
Parallelized stability screening from :Parameter High-Throughput Method Success Criteria Thermal stability Library-scale Tm measurements ≥65°C midpoint Aggregation propensity SEC-MALS under stress conditions ≤5% aggregates Serum half-life FcRn binding assays >21 days in non-human primates
Data Interpretation Frameworks
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Statistical analysis of rare antibody variant recovery (≤1:10⁴):
Apply Poisson correction: -
Benchmarking against clinically validated antibodies (e.g., MEDI4893 ):
Critical efficacy thresholds:
This framework adheres to the methodological rigor demonstrated in the provided studies while maintaining flexibility for novel antibody characterization. For AtMg00400-specific investigations, I recommend:
