AtMg00670 Antibody

Target Protein: AtMg00670

The AtMg00670 gene encodes a mitochondrial protein involved in tRNA import into plant mitochondria. Studies suggest it interacts with components of the Translocase of the Outer Mitochondrial Membrane (TOM) complex, such as Tom40 and Tom20-4 . This protein is critical for maintaining mitochondrial function under stress conditions, including heat stress .

Key Research Findings

• Mitochondrial tRNA Transport: AtMg00670 co-immunoprecipitates with Tom40 and Tom20-4, indicating its role in mitochondrial tRNA translocation .

• Heat Stress Response: Transcriptomic analyses reveal that AtMg00670 expression is upregulated during heat stress, suggesting its involvement in stress adaptation pathways .

• Antibody Validation: Specificity was confirmed via immunoprecipitation assays using recombinant AtMg00670 protein and mitochondrial lysates .

Experimental Validation and Cross-Reactivity

The antibody’s reliability was assessed using:

• Immunoprecipitation: Demonstrated interaction with Tom40 in mitochondrial extracts .

• Western Blot: Detected a single band at ~30 kDa in Arabidopsis mitochondrial fractions, consistent with the predicted molecular weight .

• Epitope Mapping: Antigen sequence coverage includes residues 50–120 of the AtMg00670 protein, ensuring minimal cross-reactivity with unrelated mitochondrial proteins .

Comparison with Related Antibodies

Technical Considerations

• Recommended Dilutions:

  • Western Blot: 1:500–1:2,000

  • Immunoprecipitation: 2–4 µg per sample

• Controls: Use mitochondrial lysates from AtMg00670 knockout mutants to confirm signal absence .

Future Research Directions

Recent advances in antigen-antibody structure prediction (e.g., HelixFold-Multimer) could refine epitope characterization for AtMg00670, enabling targeted mutagenesis studies to dissect its role in mitochondrial tRNA dynamics .