mab-7 Antibody

Definition and Target Specificity

The mab-7 Antibody refers to monoclonal antibodies designated for specific epitopes, such as:

• Influenza H7 Hemagglutinin (HA): MAb 7 (clone F39-16) binds to conserved conformational epitopes in the HA1 subunit of H7 influenza viruses, showing specificity across 14 H7 strains .

• Human Interferon-Gamma (IFN-γ): The 7-B6-1 clone targets IFN-γ for detection in immunoassays, with cross-reactivity in non-human primates .

Influenza H7 Detection

MAb 7 is integral to influenza diagnostics:

• Double-Antibody Sandwich (DAS) ELISA:

  • Capture Antibody: MAb 3 (1.6 μg/ml) coated on microplates.

  • Detection Antibody: Biotinylated MAb 7 (0.25 μg/ml) binds H7 antigen, visualized via streptavidin-HRP .

  • Sensitivity: Detects H7 strains at low viral loads (e.g., A/Turkey/Ontario/18-2/00 H7N1) .

IFN-γ Detection in Immunoassays

• ELISpot/FluoroSpot: MAb 7-B6-1 (biotinylated) pairs with capture antibodies (e.g., 1-D1K) for IFN-γ quantification .

• Cross-Reactivity: Detects IFN-γ in rhesus and cynomolgus macaques, validated in vaccine studies .

Antibody Conjugation Efficiency

In nanoparticle-based HIV prevention studies (source ), α4β7 monoclonal antibodies were tested for conjugation efficiency:

Optimal loading (43.4 μg/mg NPs) occurred at 1.4 mg/ml, enabling sustained blockage of α4β7 receptors on CD4+ T cells .

Key Research Findings

• Influenza Diagnostics: MAb 7 maintains 85–90% binding efficiency to H7 antigens after 24 hours of incubation, critical for rapid test kits .

• IFN-γ Quantification: The 7-B6-1 antibody achieves a detection limit of <10 pg/ml in ELISA, outperforming polyclonal alternatives .

• HIV Prevention: Nanoparticles conjugated with α4β7 antibodies (analogous to mab-7) reduced receptor expression by 65–75% in cervicovaginal tissues .

Limitations and Future Directions

• Influenza Variability: MAb 7 does not react with H10 subtypes, limiting pan-influenza utility .

• Cost: Therapeutic conjugates require high antibody concentrations (~1.4 mg/ml), increasing production costs .