magt1 Antibody

Overview of MAGT1 Antibody

The MAGT1 antibody (e.g., Proteintech 17430-1-AP) is a polyclonal rabbit IgG antibody targeting the MAGT1 protein. It is widely used to detect MAGT1 in techniques such as Western blot (WB), immunohistochemistry (IHC), and immunofluorescence (IF/ICC) . MAGT1 is a magnesium-selective transporter critical for cellular magnesium uptake and immune cell function, particularly in CD8+ T cells and natural killer (NK) cells .

Functional Insights

• MAGT1 deficiency is linked to XMEN disease, an X-linked immunodeficiency marked by chronic Epstein-Barr virus (EBV) infections and impaired T-cell activation .

• The antibody helps identify MAGT1 expression loss in patient cells, aiding in diagnosing XMEN and studying glycosylation defects .

Key Applications

• Immune Dysfunction Studies: MAGT1 antibodies detect protein expression in CD8+ T cells, where magnesium influx is essential for activating immune responses against viral infections .

• Glycosylation Analysis: MAGT1 is a subunit of the oligosaccharyltransferase (OST) complex. Its deficiency disrupts posttranslational glycosylation, affecting proteins like GLUT1 and SHBG .

• CRISPR/Cas9 Validation: Used to confirm MAGT1 knockout in cell lines to study magnesium-dependent glycosylation pathways .

Select Research Findings

Clinical Relevance

• Diagnostic Use: MAGT1 antibodies help diagnose XMEN by confirming MAGT1 protein absence in patient lymphocytes .

• Therapeutic Insights: Restoring MAGT1 function or magnesium supplementation may improve immune cell activity in XMEN patients .

Limitations and Considerations

• Molecular Weight Variability: Discrepancies between observed (45–47 kDa) and calculated (38 kDa) weights suggest post-translational modifications or technical artifacts .

• Tissue-Specific Expression: MAGT1 is highly expressed in immune cells, while its homolog TUSC3 compensates in non-lymphoid tissues .