max-2 Antibody

Research Applications of MAX Antibodies

MAX antibodies are employed in diverse experimental workflows to study MAX’s role in transcriptional regulation, oncogenesis, and cellular metabolism.

2.1. Transcriptional Regulation and Oncogenesis

MAX forms heterodimers with MYC to bind E-box sequences (CACGTG), activating oncogenic genes. Antibodies enable:

• Immunoprecipitation (IP): Isolation of MAX-MYC complexes to study protein-protein interactions .

• Chromatin Immunoprecipitation (ChIP): Mapping MAX binding sites to regulatory regions of target genes .

• Western Blot (WB): Quantifying MAX expression in cancer cell lines (e.g., LNCaP, PC-3) to assess oncogenic activity .

Example:

• MAX-MYC Inhibition: A MYC inhibitor (D347-2761) disrupts MAX-MYC heterodimers, reducing tumor growth in myeloma models .

2.2. Virology and Diagnostic Development

MAX antibodies contribute to viral antigen detection:

• SARS-CoV-2 Antibody Testing: MaxCyte’s electroporation technology produces high-quality RBD antigens for BLI-ISA assays to detect anti-SARS-CoV-2 antibodies .

• ELISA and Multiplex Assays: Proteintech’s recombinant MAX antibodies (e.g., 82958-2-PBS) enable high-throughput screening for MAX in complex samples .

Key Research Findings

Recent studies highlight MAX’s multifaceted roles in cellular biology and disease:

1. Tumor Suppression: MAX rewires metabolism in small cell lung cancer, acting as a tumor suppressor .

2. Epigenomic Regulation: MAX-MYC cistromes are altered by inhibitors, modulating gene expression and chromatin structure .

3. Stem Cell Differentiation: MAX regulates neuroectoderm specification in pluripotent stem cells via SOX2 activation .

4. Metabolic Regulation: MAX interacts with ChREBP to repress lncRNA Tug1 under high glucose conditions .

4.1. Immunogen and Epitope Specificity

• sc-8011 (H-2): Targets amino acids 28–151 of MAX, ensuring recognition across species .

• ab101271: Raised against a synthetic peptide spanning MAX’s C-terminus (aa 100–C-term), suitable for IHC-P .

• 10426-1-AP: Affinity-purified against MAX fusion protein Ag0680, validated for ChIP and WB .

4.2. Cross-Reactivity and Sensitivity

Notes:

• MW Variability: Observed molecular weights differ due to post-translational modifications or epitope accessibility .

• Optimal Dilution: WB dilutions range from 1:500 (10426-1-AP) to 1:1000, depending on antibody specificity .