MAZ Antibody, FITC conjugated
Description
Antibody Structure and FITC Conjugation
The MAZ antibody (Clone 133) is a mouse monoclonal IgG1 isotype raised against recombinant human MAZ protein . When conjugated with fluorescein isothiocyanate (FITC), it retains specificity for MAZ while enabling fluorescent detection. The conjugation process involves covalently linking FITC to the antibody’s lysine residues or amine groups, typically achieving a fluorophore-to-protein (F:P) ratio of 2–5 for optimal functionality .
| Parameter | Detail |
|---|---|
| Isotype | Mouse IgG1 |
| Host | Mouse |
| Immunogen | Recombinant human MAZ protein |
| FITC Conjugation | F:P ratio ~2–5; excitation/emission maxima: 490/525 nm |
Applications in Research
The MAZ Antibody, FITC conjugated, is validated for:
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Chromatin Immunoprecipitation (ChIP): Identifies MAZ binding sites on DNA, such as promoters of Sp1 family transcription factors .
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Western Blot (WB): Detects MAZ protein in lysates of breast cancer cells or skeletal muscle tissue .
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Immunofluorescence (IF): Visualizes nuclear localization of MAZ in fixed cells .
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Flow Cytometry (FCM): Quantifies MAZ expression in cell populations .
MAZ Function in Transcription
MAZ regulates transcription initiation/termination by binding G/C-rich promoter elements . Its FITC-conjugated antibody has been used to demonstrate:
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Promoter occupancy: MAZ binds ME1a1/ME1a2 sites in the c-Myc promoter, modulating transcription .
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Tumor-specific activity: Drives PPAR-γ expression in breast cancer, linking MAZ to oncogenic pathways .
Conjugation Optimization
Studies on FITC-conjugated antibodies (e.g., anti-HA Fc125) reveal that high F:P ratios (>5) reduce antibody avidity by 20–40% . For MAZ-FITC, an optimal F:P ratio of 3.5 maintains 85% functional activity .
