MYB3R1 Antibody
Description
MYB3R1: A Transcription Factor in Plant Cell Cycle Regulation
MYB3R1 belongs to the R1R2R3-Myb family of transcription factors, which modulate mitotic gene expression in plants. It functions as both an activator and repressor, depending on genetic context and cellular conditions:
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Activation: MYB3R1 binds to MSA (Mitotic Specificity element) motifs in promoters of G2/M-specific genes (e.g., AUR1, CYCB1;1) to promote cell division during processes like symbiotic infection or cytokinin signaling .
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Repression: In post-mitotic or DNA-damaged cells, MYB3R1 associates with MYB3R3/5 to inhibit mitotic genes, ensuring proper cell cycle arrest .
Key Findings:
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Act-MYBs (MYB3R1/4): Cooperate to activate >5,000 cell cycle genes in shoot meristems, with MYB3R4 showing restricted targets (~170 genes) .
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Rep-MYBs (MYB3R1/3/5): Inhibit mitotic genes in differentiated cells, preventing uncontrolled division .
Antibody-Based Research Tools
Antibodies are essential for studying MYB3R1’s DNA-binding activity and protein interactions.
Chromatin Immunoprecipitation (ChIP)
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MYB3R1-GFP/FLAG fusion proteins are used with anti-GFP or anti-FLAG antibodies to map promoter binding .
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ChIP-seq data reveal MYB3R1 binds 5,477 genes, including AUR1, CYCA3;1, and KNOLLE .
Gene Expression Analysis
Overexpression of MYB3R1 in Medicago truncatula hairy roots upregulates:
| Gene | Function | Fold Change |
|---|---|---|
| AUR1 | Aurora kinase | +2.5x |
| CYCA3;1 | Cyclin A | +3.0x |
| KNOLLE | Vesicle fusion during cytokinesis | +1.8x |
Source:
Functional Comparison with MYB3R4
Table 2 highlights key differences between MYB3R1 and MYB3R4:
| Feature | MYB3R1 | MYB3R4 |
|---|---|---|
| Subcellular Localization | Constitutively nuclear | Cytokinin-responsive nuclear accumulation |
| Target Genes | >5,000 (broad activation) | ~170 (restricted activation) |
| LCYEPP Motif | Present | Absent |
| Symbiotic Role | Activates AUR1 during rhizobial infection | Limited role in symbiosis |
Source:
Applications in Agriculture and Plant Biology
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Symbiotic Nodulation: MYB3R1 overexpression enhances rhizobial infection in legumes by promoting cell division in root hairs .
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Meristem Regulation: Dual role in shoot meristems—activating proliferation under cytokinin and repressing division post-mitosis .
Future Directions
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Antibody Optimization: Development of high-affinity antibodies for precise MYB3R1 detection in diverse tissues.
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Synthetic Biology: Engineering MYB3R1 variants to control cell cycle genes for crop yield improvement.
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- MYB3R1 and MYB3R4 are thought to have diverse roles during plant development by regulating G2/M-specific genes with various functions, as well as genes potentially unrelated to the cell cycle. PMID: 21862669
- Research suggests that the structurally related MYB3R1 and MYB3R4 transcription factors may positively regulate cytokinesis primarily through transcriptional activation of the KN gene. PMID: 17287251
