MBP Mouse Antibody

Maltose-Binding protein, Antibody
Shipped with Ice Packs
In Stock
Cat. No.
BT24642
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Applications in Research and Diagnostics

MBP Mouse Antibodies are validated for multiple experimental techniques:

ApplicationRecommended DilutionSample TypesKey Findings from Studies
Western Blot (WB)1:5,000–10,000 Human/mouse/rat brain homogenates Detects bands at 15–22 kDa
Immunohistochemistry1:500–10,000 Frozen/paraffin-embedded CNS tissue Localizes myelinated fibers
Immunocytochemistry8–25 µg/mL Oligodendrocyte cultures Identifies MBP in cell cytoplasm
ELISA1:500–1,000 MS patient sera Detects IgM/IgG anti-MBP autoantibodies

Role in Multiple Sclerosis (MS)

  • Anti-MBP antibodies in MS patients show 84–100% seropositivity in relapsing-remitting cases .

  • Peptides MBP(76–116) and MBP(81–106) demonstrate high IgM reactivity in MS sera, with IC₅₀ values of 2.2×10⁻⁷ and 8.4×10⁻⁷, respectively .

  • Helical structures in MBP peptides (residues 87–96) enhance antibody recognition in solid-phase assays .

Cognitive Decline Post-Stroke

  • Elevated MBP antibody titers (≥95th percentile) correlate with post-stroke cognitive decline (OR = 9.02, p = 0.03) .

Experimental Autoimmune Encephalomyelitis (EAE)

  • MBP immunization induces EAE in mice, mimicking MS pathology .

Technical Considerations

  • Fixation sensitivity: Prolonged fixation reduces antibody binding; 2–3 hours optimal for IHC .

  • Antigen retrieval: Citrate buffer treatment recommended for paraffin sections .

  • Storage: Stable at -70°C for 12 months; avoid freeze-thaw cycles post-reconstitution .

Limitations and Controversies

  • Variable reported seropositivity (0–100%) for anti-MBP antibodies in MS cohorts .

  • Non-specific binding observed in IgM-dominant assays due to pentameric avidity .

Product Specs

Introduction
Maltose Binding Protein (MBP) is a key component of the maltose/maltodextrin system in Escherichia coli, responsible for the uptake and breakdown of maltodextrins. This intricate system comprises numerous proteins and protein complexes. MalE, also known as MBP, specifically binds to maltodextrins after they diffuse through the LamB pore in the outer membrane and enter the periplasm. This binding prevents the sugar from diffusing back out. Subsequently, MalE transfers the sugar to a complex of MalF and MalG located in the cytoplasmic membrane. Here, the ATPase MalK facilitates active transport. With a molecular weight of 40,622 daltons, MBP consists of two domains with similar secondary structures, each characterized by central pleated sheets surrounded by helices. These domains are connected by three bridges, forming a deep groove where the sugar binds. All hydroxyl groups of the sugar establish hydrogen bonds with the protein's side chains, with water molecules also participating in this network.
Applications
**Immunofluorescence:** For optimal results on frozen sections, use a dilution of 1:200 and incubate with the primary antibody for 60 minutes at 25°C. **Western Blotting:** A working dilution of 1:500 is recommended for Western blotting. Incubate with the primary antibody for 60 minutes at 25°C.
Stability
To maintain optimal performance, store the unopened antibody at a temperature of 4°C or lower. Under these conditions, the product is expected to remain stable for one year from the purchase date. Once reconstituted, the antibody should be stored at 4°C and is stable for a minimum of two months. For long-term storage, freezing aliquots of the antibody at -20°C is recommended. However, avoid using frost-free freezers for this purpose. Repeated cycles of freezing and thawing should be avoided. It is best practice to prepare working dilutions fresh on the day of use.
Specificity
Mouse monoclonal antibody against the amino acid stretch of KDPRIAA (the amino acid residue 339 to 345) of the periplasmic maltose-binding protein (MBP) [Escherichia coli] (Accession #: AAB59056).
Type
Mouse Antibody Monoclonal.
Immunogen
The malE gene is inserted in a pMAL vector (Maina et al., 1988) and then expressed in E. coli JM109.
The purified recombinant MBP protein was then used for mouse immunization.
Storage Buffer
Theammonium sulfate-precipitated ascites fluid is in PBS + 1% BSA & then lyophilized.

Product Science Overview

Antibodies

Antibodies, also known as immunoglobulins (Ig), are large, Y-shaped proteins used by the immune system to identify and neutralize antigens such as bacteria and viruses . Each antibody binds to a specific antigen in a highly specific interaction analogous to a lock and key .

Structure and Function

Antibodies are composed of two identical light (L) chains and two identical heavy (H) chains, which are held together by disulfide bonds to form a flexible Y shape . Each chain is composed of a variable (V) region and a constant © region . The tips of the Y-shaped structure contain paratopes that specifically bind to epitopes on antigens, allowing the two molecules to bind together with precision .

Classes and Functions

Antibodies occur in five classes, sometimes called isotypes: IgA, IgD, IgE, IgG, and IgM . Each class has different functions and is released in different parts of the body at various stages of an immune response . For example, IgG is the most common antibody in blood and extracellular fluid, providing the majority of antibody-based immunity against invading pathogens .

Medical Applications

Antibodies are crucial for the immune response and are used in various medical applications, including diagnostics and therapeutics . Monoclonal antibodies, which are identical antibodies produced by a single clone of cells, are used to treat diseases such as cancer and autoimmune disorders .

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