MDC Human, His
Description
Introduction to MDC Human, His
MDC Human, His (Macrophage-Derived Chemokine with a C-terminal His-Tag) is a recombinant protein engineered for research and therapeutic applications. It belongs to the CC chemokine family (CCL22) and is primarily expressed by dendritic cells, macrophages, and activated monocytes . The His-Tag facilitates purification via metal affinity chromatography, enhancing yield and stability .
Functional Roles and Mechanisms
MDC Human, His binds to CCR4, a G-protein-coupled receptor expressed on Th2 lymphocytes, regulatory T cells (Tregs), and dendritic cells . Key functions include:
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Immune Cell Recruitment: Chemotaxis of monocytes, dendritic cells, and IL-2-activated natural killer cells .
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Th2/Treg Modulation: Critical for Th2 cell migration during allergic responses and Treg recruitment in autoimmune diseases .
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Pathophysiological Roles: Elevated in atopic dermatitis and linked to tumor-associated immune suppression via Treg recruitment .
Neutralization Studies
Clinical Relevance
Product Specs
MGSSHHHHHH SSGLVPRGSH MGPYGANMED SVCCRDYVRY RLPLRVVKHF YWTSDSCPRP GVVLLTFRDK EICADPRVPW VKMILNKLSQ.
Product Science Overview
Macrophage-Derived Chemokine (MDC), also known as CCL22, is a chemokine belonging to the C-C subfamily. Chemokines are small cytokines or signaling proteins secreted by cells, and they play a crucial role in immune responses by directing the movement of circulating leukocytes to sites of inflammation or injury.
CCL22 was initially discovered in macrophages, but it is also produced by activated B lymphocytes and dendritic cells . In humans, the gene encoding CCL22 is located on chromosome 16q13, in close proximity to the gene for another chemokine, CCL17 . This region forms a mini-cluster of chemokine genes that have both homeostatic and anti-inflammatory functions.
The human recombinant form of CCL22, often tagged with a His (histidine) tag for purification purposes, is a non-glycosylated polypeptide chain containing 90 amino acids and has a molecular mass of approximately 10.3 kDa . The His tag facilitates the purification of the protein using metal affinity chromatography.
CCL22 is involved in various immune processes, including the maturation and migration of T-cells . It exerts its effects by binding to the chemokine receptor CCR4, which is expressed on the surface of target cells . This interaction plays a significant role in the chemotaxis (movement) of immune cells, particularly in the context of inflammatory responses.
CCL22 has been implicated in several physiological and pathological processes. It is known to be involved in the immune response to viral infections, including COVID-19 . Research has shown that plasma levels of CCL22 tend to be significantly depleted in patients with acute viral infections compared to healthy individuals . This depletion may be due to the binding of viral products to CCL22, blocking its activity, or the inhibition of dendritic cell function.
The recombinant form of CCL22, tagged with His, is widely used in research to study its role in immune responses and its potential therapeutic applications. For instance, it is used to investigate the mechanisms of T-cell migration and the regulation of immune responses in various diseases, including autoimmune disorders and cancers.
