At1g73050 Antibody

Expression Profile in Anther Development

At1g73050 was identified in a transcriptomic study comparing wild-type and cdm1 mutant Arabidopsis anthers:

• Expression Reduction: At1g73050 exhibited a 1.82-fold downregulation in cdm1 mutants during late anther developmental stages (stages 8–12) .

• Functional Context: The gene is implicated in callose dissolution, a critical process during microsporogenesis. Callose, a β-1,3-glucan polymer, forms transient cell walls around pollen mother cells and tetrads. Dysregulation in cdm1 mutants disrupts callose metabolism, leading to male sterility .

Potential Applications

1. Developmental Biology:

   • Study pollen development and male gametophyte formation in Arabidopsis.

   • Investigate regulatory networks involving callose synthase genes (e.g., CalS5).

2. Protein Localization:

   • Map spatial expression patterns of At1g73050 protein in plant tissues using immunohistochemistry or fluorescent tagging.

3. Mutant Phenotyping:

   • Characterize knockout or overexpression lines to assess morphological or reproductive defects.

Technical Considerations

• Cross-Reactivity: No cross-reactivity with non-target plant species has been reported, but validation in non-Arabidopsis systems is advised.

• Storage: Stable at -20°C for long-term storage; avoid repeated freeze-thaw cycles .