MED4 Antibody, FITC conjugated
Description
Applications and Reactivity
This antibody is validated for:
Reactivity:
Immunogen and Target
The antibody targets the MED4 protein (UniProt ID: Q9NPJ6), a 36 kDa subunit of the Mediator complex . Key features include:
Biological Role
MED4 is a critical component of the Mediator complex, which bridges gene-specific transcription regulators and RNA polymerase II. It facilitates the assembly of preinitiation complexes for RNA polymerase II-dependent gene transcription . The FITC conjugation enables fluorescence-based detection in assays like flow cytometry and imaging .
Usage Guidelines
| Application | Dilution Range |
|---|---|
| Western Blot (WB) | 1:300–5,000 |
| Immunofluorescence (IF) | 1:50–200 |
Critical Notes:
Conjugation and FITC Properties
FITC (fluorescein isothiocyanate) emits green fluorescence (524 nm) upon excitation at 495 nm. Its conjugation to antibodies enables high-resolution detection in fluorescence microscopy and flow cytometry . MED4-FITC’s design ensures minimal steric interference with antigen binding, a key factor in maintaining assay specificity .
Product Specs
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Target Background
- Retinoblastoma RB1 -/- cells are unable to survive in the absence of MED4, both in vitro and in orthotopic xenograft models in vivo. This identifies MED4 as a crucial survival gene in retinoblastoma. PMID: 24858910
- Research suggests that Vitamin D Receptor polymorphisms may be associated with nasal carriage of S. aureus in individuals with type 1 diabetes. PMID: 19411183
