MEG6 Antibody

Basic Research Questions

• What are the primary applications of M6 antibody in neuroscience research?

  M6 antibody is primarily used for studying neuronal development and extension. It binds to the surface of murine central nervous system neurons and certain epithelial cells (choroid plexus and kidney proximal tubules). The antibody can be applied in neuronal culture studies to investigate neurite extension mechanisms, as it has been shown to interfere with this process without initially affecting cell viability. Applications include immunohistochemistry, culture-based assays, and developmental neurobiology studies focusing on embryonic neural development from as early as embryonic day 10 .

• What is the MAGEA6 antigen and why is it significant in cancer research?

  MAGEA6 (Melanoma Antigen Family A, 6) is a 35 kDa glycoprotein (observed molecular weight 45-50 kDa) belonging to the MAGE family of tumor antigens. Its significance in cancer research stems from its restricted expression pattern - primarily in malignant tumors with limited expression in normal tissues (restricted to germ cells of testis, fetal ovary, and placenta). This unique expression profile makes MAGEA6 an ideal target for cancer immunotherapy and a potential biomarker for detection and monitoring of various cancers, particularly melanoma .

• How should MAGEA6 antibodies be stored and prepared for experimental use?

  MAGEA6 antibodies should be stored at -20°C in their lyophilized state where they remain stable for approximately one year after shipment. For optimal performance:

  • Reconstitute following the specific protocol provided in the Certificate of Analysis

  • After reconstitution, store with proper buffer conditions (typically PBS with 0.02% sodium azide and 50% glycerol at pH 7.3)

  • Aliquot to avoid repeated freeze-thaw cycles which can compromise antibody functionality

  • For long-term storage, maintain at -20°C in the appropriate buffer

• What are the recommended dilutions for different applications of MAGEA6 antibody?

  Application	Recommended Dilution
  Western Blot (WB)	1:500-1:3000
  Immunohistochemistry (IHC)	1:50-1:500
  Immunofluorescence (IF)/ICC	1:200-1:800

  Note: It is recommended to titrate these antibodies in each specific testing system to obtain optimal results as performance can be sample-dependent .