mfsd6l Antibody

Overview of MFSD6L Protein

MFSD6L is a 586-amino acid transmembrane protein belonging to the Major Facilitator Superfamily (MFS), which facilitates small solute transport via chemiosmotic gradients . Key features include:

• Molecular Weight: 64 kDa (precursor), increasing to 72 kDa post-glycosylation .

• Structure: 11 transmembrane domains and two MFS-specific regions (residues 28–368) .

• Expression: Highly expressed in glandular tissues (pancreas, thyroid) and upregulated under glucose starvation .

• Function: Predicted role in antigen processing via MHC class I interactions, with potential disease links to viral encephalitis .

Development and Suppliers of MFSD6L Antibodies

Anti-MFSD6L antibodies are produced by multiple suppliers, with variations in applications, reactivity, and formats.

Key Applications in Research

MFSD6L antibodies are utilized in diverse experimental workflows:

Table 2: Common Applications and Protocols

Research Findings Using MFSD6L Antibodies

• Subcellular Localization: Confirmed membrane localization via WB and immunofluorescence .

• Post-Translational Modifications: Detected glycosylation at residues N110, N129, and N224 using glycosidase-treated lysates .

• Disease Association: Elevated MFSD6L expression observed in glucose-starved cancer cells, suggesting metabolic adaptation roles .

Validation and Quality Control

Proteintech’s antibody (27730-1-AP) exemplifies rigorous validation:

• Specificity: Recognizes a single band at 64 kDa in human cell lines .

• Cross-Reactivity: No reactivity with mouse or rat orthologs .

• Storage: Stable at -20°C for one year in 50% glycerol .

Future Directions

• Functional Studies: Role in MHC class I antigen presentation and immune evasion .

• Therapeutic Potential: Targeting MFSD6L in metabolic disorders or viral infections .