mfsd8 Antibody

Introduction to MFSD8 Antibody

MFSD8 antibodies are immunological reagents designed to detect and analyze the MFSD8 protein, a lysosomal membrane transporter linked to neuronal ceroid lipofuscinosis (NCL) and frontotemporal lobar degeneration (FTLD) . These antibodies enable researchers to investigate MFSD8's role in autophagy, lysosomal chloride ion transport, and disease pathogenesis .

Key Research Applications

MFSD8 antibodies have been utilized in:

• Disease Mechanism Studies: Identifying MFSD8's association with NCL and FTLD through post-mortem brain tissue analysis .

• Viral Entry Research: Demonstrating MFSD8's involvement in SARS-CoV-2 infection by modulating lipid raft composition .

• Functional Characterization: Assessing lysosomal enzyme activity, protein secretion, and cell proliferation in Dictyostelium discoideum models .

Specificity Validation

• Knockdown Confirmation: The HPA044802 antibody (ATLAS Antibodies) showed near-complete loss of signal in MFSD8-knockdown cells, confirming specificity .

• HA-Tagged Constructs: Anti-HA antibodies were used to detect overexpressed HA-MFSD8 in COS-1 cells, aiding subcellular localization studies .

Functional Insights

• Lysosomal Dysregulation: MFSD8 deficiency in HEK293T cells reduced SARS-CoV-2 viral load by 90%, linked to altered GM1 ganglioside levels .

• Developmental Roles: In D. discoideum, MFSD8 loss increased lysosomal enzyme activity and disrupted cell adhesion/secretion pathways .

Technical Considerations for Use

• Antigen Retrieval: For IHC, use TE buffer (pH 9.0) or citrate buffer (pH 6.0) .

• Controls: Include knockdown/knockout samples (e.g., MFSD8-deficient HEK293T cells ) to validate antibody performance.

• Cross-Reactivity: Verify species compatibility; the 24298-1-AP antibody reacts with human and mouse samples .