MMP9 Rat
Description
Introduction to MMP9 in Rats
Matrix metalloproteinase-9 (MMP-9), also known as gelatinase B, is a zinc-dependent endopeptidase critical for extracellular matrix (ECM) remodeling. In rats, MMP-9 plays pivotal roles in physiological processes and pathological conditions, including cardiovascular remodeling, neuroinflammation, and cancer progression. Its expression is tightly regulated, and dysregulation is implicated in diseases such as atherosclerosis, traumatic brain injury (TBI), and stroke .
Cardiovascular System
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Vascular Remodeling: Overexpression in Fischer rats enhances smooth muscle cell (SMC) migration, leading to luminal dilation and reduced extracellular matrix content post-injury .
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Atherosclerosis and Aneurysms: Elevated MMP-9 activity promotes plaque instability and aneurysm formation. Inhibition via doxycycline reduces aortic inflammation and aneurysm growth .
Neurological Disorders
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Cerebral Ischemia: MMP-9 expression peaks 24 hours post-middle cerebral artery occlusion (MCAO), localizing to endothelial cells and neutrophils. Inhibition reduces infarct size .
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Traumatic Brain Injury (TBI): MMP-9 levels rise post-injury, correlating with edema and neuronal damage. Minocycline administration reduces MMP-9 by 9.5% in Wistar rats, mitigating injury severity .
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Epilepsy: MMP-9 knockout rats exhibit reduced seizure susceptibility, while overexpression accelerates epileptogenesis .
Cancer and Metastasis
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Tumor Progression: MMP-9 facilitates angiogenesis and ECM degradation, promoting metastasis. Its activity is modulated by chemokine cleavage (e.g., CXCL12 degradation) .
Research Findings from Rat Models
Therapeutic Interventions Targeting MMP9 in Rats
Comparative Analysis: Human vs. Rat MMP9
| Feature | Human MMP9 | Rat MMP9 |
|---|---|---|
| Pro-domain | Cysteine residue (binds lipocalin) | Serine residue (altered binding) |
| Linker Domain | Shorter, less glycosylation | Longer, 23 additional amino acids |
| Molecular Weight | 92 kDa (pro), 88 kDa (active) | 105 kDa (pro), 95 kDa (active) |
| Neurological Role | Synaptic remodeling, neuroinflammation | Epileptogenesis, TBI recovery |
Product Specs
Matrix metalloproteinase-9, MMP-9, 92 kDa gelatinase, Gelatinase B, GELB, MMP9, CLG4B.
HEK293 Cells.
APHQRQPTYV VFPRDLKTSN LTDTQLAEDY LYRYGYTRAA QMMGEKQSLR PALLMLQKQL SLPQTGELDS ETLKAIRSPR CGVPDVGKFQ TFEGDLKWHH HNITYWIQSY TEDLPRDVID DSFARAFAVW SAVTPLTFTR VYGLEADIVI QFGVAEHGDG YPFDGKDGLL AHAFPPGPGI QGDAHFDDDE LWSLGKGAVV PTYFGNANGA PCHFPFTFEG RSYLSCTTDG RNDGKPWCGT TADYDTDRKY GFCPSENLYT EHGNGDGKPC VFPFIFEGHS YSACTTKGRS DGYRWCATTA NYDQDKLYGF CPTRADVTVT GGNSAGEMCV FPFVFLGKQY STCTGEGRSD GRLWCATTSN FDADKKWGFC PDQGYSLFLV AAHEFGHALG LDHSSVPEAL MYPMYHYHED SPLHEDDIKG IQHLYGRGSK PDPRPPATTA AEPQPTAPPT MCPTAPPMAY PTGGPTVAPT GAPSPGPTGP PTAGPSEAPT ESSTPVDNPC NVDVFDAIAD IQGALHFFKD GRYWKFSNHG GSQLQGPFLI ARTWPALPAK LNSAFEDPQS KKIFFFSGRK MWVYTGQTVL GPRSLDKLGL GSEVTLVTGL LPRRGGKALL ISRERIWKFD LKSQKVDPQS VTRLDNEFSG VPWNSHNVFH YQDKAYFCHD KYFWRVSFHN RVNQVDHVAY VTYDLLQCPH HHHHH.
Product Science Overview
Matrix Metalloproteinase-9 (MMP-9), also known as gelatinase B, is a member of the matrix metalloproteinase (MMP) family. These enzymes are zinc and calcium-dependent endopeptidases capable of degrading various components of the extracellular matrix (ECM), such as collagen, elastin, and proteoglycans . MMP-9 is particularly notable for its role in both physiological and pathological processes, including tissue remodeling, inflammation, and cancer metastasis .
MMP-9 is initially produced as an inactive zymogen (proMMP-9) that requires activation to become enzymatically functional . The activation process involves the cleavage of the pro-domain, which masks the catalytic site of the enzyme. This cleavage can be mediated by other proteases, oxidative modifications, or conformational changes due to substrate binding .
MMP-9 plays a crucial role in various biological processes:
- Tissue Remodeling: MMP-9 is involved in the degradation of ECM components, facilitating tissue remodeling and repair .
- Inflammation: MMP-9 is upregulated during inflammatory responses and contributes to the migration of leukocytes to the site of inflammation by degrading basement membrane components .
- Neuroinflammation and Cerebral Ischemia: MMP-9 is actively involved in blood-brain barrier disruption following cerebral ischemia and neuroinflammation .
- Synaptic Plasticity and Memory: In the brain, MMP-9 is required for hippocampal late-phase long-term potentiation (L-LTP) and memory formation .
MMP-9 is implicated in several pathological conditions:
- Cancer: MMP-9 facilitates cancer cell invasion and metastasis by degrading ECM barriers .
- Cardiovascular Diseases: MMP-9 contributes to the atherogenic environment of the vessel wall and is involved in the regulation of cytokine production and macrophage function .
- Bone Diseases: MMP-9 plays a regulatory role in osteoclastogenesis, linking cardiovascular diseases and bone disorders in high-fat diet-fed rats .
Recombinant MMP-9 (Rat) is a laboratory-produced version of the enzyme, often used in research to study its functions and interactions. The recombinant form allows for controlled experiments to understand the enzyme’s role in various biological and pathological processes.
